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1.
J Clin Aesthet Dermatol ; 15(10): 52-57, 2022 Oct.
Article in English | MEDLINE | ID: mdl-36312825

ABSTRACT

Background: Obesity became a worldwide public health problem and its treatment presents a strict relationship with skin flaccidity, for which the development of non-invasive therapies is an emerging field. Objective: This study aims to evaluate the physiological response of the skin tissue of individuals with obesity with flaccidity to the physiological stimulus of shockwave therapy (ESWT). Methods: This is a comparative intervention study based on histological and immunohistochemical analyses of a set of samples of skin tissue of women with Grade II obesity who achieved a 10 percent preoperative weight loss before bariatric surgery and complaints of skin flaccidity, subjected to the ESWT treatment. A total of seven sessions were carried out in the abdominal region on the left side, and the collateral side was used as control; the biological material was collected at the moment of the bariatric surgery. Hematoxylin and Eosin, Masson's trichrome, Picrosirius Red and the markers for immunohistochemical were used for the morphological evaluation. Results: Fourteen women were included in the research. The results demonstrated that the tissue which underwent the ESWT intervention presented significant increases of fibroblast cells (p<0.0001) and collagen fibers Type I and II (p<0.0002). In the significant expressions of the markers FGF1, FGF2, FGFR1 were identified in the exposed side (p<0.0002, 0.0017, <0.0001, respectively) as well as a significantly higher expression of Ki67 marker of cell proliferation (p<0.0002). Conclusion: ESWT was associated with a significant increase of cell proliferation and collagen expression in flaccid skin of individuals who achieved weight loss before bariatric surgery.

2.
Acta Cir Bras ; 29(9): 544-52, 2014 Sep.
Article in English | MEDLINE | ID: mdl-25252200

ABSTRACT

PURPOSE: To evaluate the effects of sucralfate on tissue content of neutral and acids mucins in rats with diversion colitis. METHODS: Thirty-six rats were submitted to a proximal right colostomy and a distal mucous fistula. They were divided into two groups according to sacrifice to be performed two or four weeks after intervention. Each group was divided into three subgroups according daily application of enemas containing saline, sucralfate at 1.0 g/kg/day or 2.0 g/kg/day. Colitis was diagnosed by histological analysis and neutral and acid mucins by Periodic Acid Schiff and Alcian Blue techniques, respectively. The contents of mucins were quantified by computer-assisted image analysis. Student's t paired and ANOVA test were used to compare the contents of both types of mucins among groups, and to verify the variance with time, establishing level of signification of 5% for both (p<0.05). RESULTS: Enemas containing sucralfate improves the inflammation and increases the tissue contents of neutral and acid mucins. The content of neutral mucins does not change with the time or concentration of sucralfate used, while acid mucins increases with concentration and time of intervention. CONCLUSIONS: Sucralfate enemas improve the inflammatory process and increase the tissue content of neutral and acid mucins in colon without fecal stream.


Subject(s)
Anti-Ulcer Agents/therapeutic use , Colitis/drug therapy , Enema/methods , Membrane Glycoproteins/analysis , Mucins/analysis , Sucralfate/therapeutic use , Animals , Anti-Ulcer Agents/pharmacology , Colitis/pathology , Colon/drug effects , Colon/pathology , Disease Models, Animal , Image Processing, Computer-Assisted , Intestinal Mucosa/drug effects , Intestinal Mucosa/pathology , Male , Mucins/drug effects , Rats, Wistar , Reproducibility of Results , Sucralfate/pharmacology , Time Factors , Treatment Outcome
3.
Acta cir. bras ; 29(9): 544-552, 09/2014. graf
Article in English | LILACS | ID: lil-722131

ABSTRACT

PURPOSE: To evaluate the effects of sucralfate on tissue content of neutral and acids mucins in rats with diversion colitis. METHODS: Thirty-six rats were submitted to a proximal right colostomy and a distal mucous fistula. They were divided into two groups according to sacrifice to be performed two or four weeks after intervention. Each group was divided into three subgroups according daily application of enemas containing saline, sucralfate at 1.0 g/kg/day or 2.0 g/kg/day. Colitis was diagnosed by histological analysis and neutral and acid mucins by Periodic Acid Schiff and Alcian Blue techniques, respectively. The contents of mucins were quantified by computer-assisted image analysis. Student's t paired and ANOVA test were used to compare the contents of both types of mucins among groups, and to verify the variance with time, establishing level of signification of 5% for both (p<0.05). RESULTS: Enemas containing sucralfate improves the inflammation and increases the tissue contents of neutral and acid mucins. The content of neutral mucins does not change with the time or concentration of sucralfate used, while acid mucins increases with concentration and time of intervention. CONCLUSIONS: Sucralfate enemas improve the inflammatory process and increase the tissue content of neutral and acid mucins in colon without fecal stream. .


Subject(s)
Animals , Male , Anti-Ulcer Agents/therapeutic use , Colitis/drug therapy , Enema/methods , Membrane Glycoproteins/analysis , Mucins/analysis , Sucralfate/therapeutic use , Anti-Ulcer Agents/pharmacology , Colitis/pathology , Colon/drug effects , Colon/pathology , Disease Models, Animal , Image Processing, Computer-Assisted , Intestinal Mucosa/drug effects , Intestinal Mucosa/pathology , Mucins/drug effects , Rats, Wistar , Reproducibility of Results , Sucralfate/pharmacology , Time Factors , Treatment Outcome
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