Demonstration of immuno-bound alkaline phosphatase by a direct lead method. Optimization of reaction parameters by microdensitometry using computer-aided image processing.

Antigens labelled by the immunohistochemical alkaline phosphatase anti-alkaline phosphatase (APAAP) method can be visualized by a lead capture technique. Optimal conditions of the reaction, evaluated by microdensitometry using computer-aided image processing, are described.