Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
J Biol Chem ; 276(16): 13433-41, 2001 Apr 20.
Article in English | MEDLINE | ID: mdl-11278567

ABSTRACT

The variable V1V2 and V3 regions of the human immunodeficiency virus type-1 (HIV-1) envelope glycoprotein (gp120) can influence viral coreceptor usage. To substantiate this we generated isogenic HIV-1 molecularly cloned viruses that were composed of the HxB2 envelope backbone containing the V1V2 and V3 regions from viruses isolated from a patient progressing to disease. We show that the V3 amino acid charge per se had little influence on altering the virus coreceptor phenotype. The V1V2 region and its N-linked glycosylation degree were shown to confer CXCR4 usage and provide the virus with rapid replication kinetics. Loss of an N-linked glycosylation site within the V3 region had a major influence on the virus switching from the R5 to X4 phenotype in a V3 charge-dependent manner. The loss of this V3 N-linked glycosylation site was also linked with the broadening of the coreceptor repertoire to incorporate CCR3. By comparing the amino acid sequences of primary HIV-1 isolates, we identified a strong association between high V3 charge and the loss of this V3 N-linked glycosylation site. These results demonstrate that the N-linked glycosylation pattern of the HIV-1 envelope can strongly influence viral coreceptor utilization and the R5 to X4 switch.


Subject(s)
CD4-Positive T-Lymphocytes/virology , HIV Envelope Protein gp120/chemistry , HIV Envelope Protein gp120/physiology , HIV-1/physiology , Receptors, CCR5/physiology , Receptors, CXCR4/physiology , Receptors, HIV/physiology , Amino Acid Sequence , Amino Acid Substitution , CD4-Positive T-Lymphocytes/immunology , Chimera , Cloning, Molecular , Glycosylation , HIV Envelope Protein gp120/genetics , HIV-1/genetics , Humans , Kinetics , Molecular Sequence Data , Mutagenesis, Site-Directed , Phenotype , Virus Replication
SELECTION OF CITATIONS
SEARCH DETAIL
...