ABSTRACT
A test is described by means of which formation of mannitol from fructose by lactic acid bacteria can be readily detected. The test is based on removal of interference of residual fructose by dehydration with hydrochloric acid followed by thin-layer chromatography.
Subject(s)
Bacteriological Techniques , Lactobacillaceae/metabolism , Mannitol/biosynthesis , Chromatography, Thin Layer , Fructose/metabolism , Hydrochloric Acid , Lactobacillaceae/classification , Lactobacillus/metabolism , Leuconostoc/metabolismABSTRACT
A bacterium capable of rapid growth on methanol as sole carbon source was isolated and classified as a new pseudomonad. Its doubling time was about 100 min at 32 to 37 C, and it grew well at methanol concentrations up to 2%. The organism was sensitive to phosphate, but reasonable cell densities could be obtained by using pH control. Cell yields of about 31%, based on methanol consumed, were obtained. The amino acid pattern of the protein indicated that the bacterium holds promise as a source of single-cell protein.
