ABSTRACT
Experiments were carried out to investigate effects of heavy metal salts and phenol both alone and in different combinations on respiration rate and coupling of respiration and oxidative phosphorylation by rat liver mitochondria. Oxygen consumption measurements were obtained using a Clark electrode and lactate plus glutamate as substrates. It is shown that a 50% decrease of the respiratory rate was achieved in less concentrations of substances in the mixture compared with the effects of the individual substances. For respiratory control recovery by EDTA titration more amounts of complexion for model mixtures was necessary. The results evidence synergism of the substances responsible for a considerable increase in toxicity. Mitochondrial test-systems are suggested for rapid prognosis of toxicity of multicomponent mixtures of substances.
Subject(s)
Metals/toxicity , Mitochondria, Liver/drug effects , Phenols/toxicity , Animals , Drug Combinations , Drug Synergism , Male , Metals/administration & dosage , Mitochondria, Liver/metabolism , Oxidative Phosphorylation , Oxygen Consumption , Phenol , Phenols/administration & dosage , Rats , Rats, Wistar , SaltsABSTRACT
The effect of toxic substances on the functional state of liver mitochondria has been studied. It has been shown, that the contact of toxic substances with mitochondria lead to the reduction of the degree of association of oxidation with phosphorylation. EDTA in concentrations necessary to inhibit lipid peroxide oxidation prevents toxic effects. Preliminary antioxidant treatment of mitochondria prevents toxic damage of membranes. A close correlation has been established between chemical compounds toxicity and the concentration of antioxidants necessary for the prevention of toxic effects, as well as between chemical compounds toxicity and cumulation of malonic dialdehyde in mitochondria membranes.
Subject(s)
Chemical and Drug Induced Liver Injury/etiology , Disease Models, Animal , Edetic Acid/pharmacology , Lipid Peroxidation/physiology , Mitochondria, Liver/metabolism , Solvents/toxicity , Acute Disease , Animals , Antioxidants/pharmacology , Chemical and Drug Induced Liver Injury/pathology , Chemical and Drug Induced Liver Injury/prevention & control , In Vitro Techniques , Lipid Peroxidation/drug effects , Male , Mitochondria, Liver/drug effects , Oxidative Phosphorylation/drug effects , RatsABSTRACT
Experiments on mice and rats have disclosed that taftsin so far known for its stimulatory action on phagocytosis has psychotropic properties. Taftsin potentiates the motor and orienting activity of the animals, reduces the effects of hexenal, reserpine, potentiates the action of amphetamine and arecoline. It shortens the latent period and time of the reaction during training of the rats in a T-shaped maze, but does not increase the number of the training animals. The effect of taftsin has a biphasic nature and seems likely to be related to the effect on alpha-adrenergic systems of the brain.
