ABSTRACT
Active antisera containing antibodies to deproteinized DNA preparations of mouse normal and leukemic tissues have been obtained. Examination of antisera by the quantitative complement fixation test with the corresponding test antigens has demonstrated immunological specificity of DNA preparations isolated from the organs of mice with leukemia induced by oncornaviruses. The DNA specificity in question is inherent not only in the native but also in the thermodenatured molecule of DNA.
Subject(s)
DNA, Neoplasm/immunology , Leukemia, Experimental/immunology , Animals , Complement Fixation Tests , Epitopes/analysis , Friend murine leukemia virus , Mice , Nucleic Acid Conformation , Nucleic Acid Denaturation , Rauscher Virus , SpleenABSTRACT
Active antisera containing antibodies to deproteinized DNA preparations of normal tissue and the spleen of cows suffering from myeloleukosis were obtained. The anti DNA sera to DNA preparations contained complement fixing antibodies related to gamma M globulins. In the study of leukosis and normal anti DNA sera in quantitative CFR with the corresponding test antigens, immunological specificity of DNA preparations isolated from the organs of cows affected with myeloleukosis was established. Immunological specificity of leukosis DNA was confirmed in tests with the absorption of leukosis anti DNA sera by DNA preparations of homologous normal tissues. This specificity is an inherent quality of not only the native but also the heat-denatured DNA molecule.
