ABSTRACT
Three triterpene glycosides were isolated from the roots of Weigela florida "rumba" (Bunge) A. DC.: two previously undescribed 3-O-ß-d-xylopyranosyl-(1â2)-[ß-d-xylopyranosyl-(1â4)]-ß-d-xylopyranosyl-(1â4)-ß-d-xylopyranosyl-(1â3)-α-L-rhamnopyranosyl-(1â2)-α-l-arabinopyranosyloleanolic acid (1) and 3-O-ß-d-xylopyranosyl-(1â2)-[ß-d-glucopyranosyl-(1â4)]-ß-d-xylopyranosyl-(1â4)-ß-d-xylopyranosyl-(1â3)-α-l-rhamnopyranosyl-(1â2)-α-L-arabinopyranosyloleanolic acid (2), and one isolated for the first time from a natural source 3-O-ß-d-xylopyranosyl-(1â3)-α-l-rhamnopyranosyl-(1â2)-α-l-arabinopyranosyloleanolic acid (3). Their structures were elucidated mainly by 2D NMR spectroscopic analysis (COSY, TOCSY, NOESY, HSQC, HMBC) and mass spectrometry. Compounds 2 and 3 were further evaluated as antigens in enzyme-linked immunosorbent assay (ELISA) to recognize IgM antibodies in multiple sclerosis (MS) patients' sera.