1.
J Clin Microbiol
; 40(12): 4771-5, 2002 Dec.
Article
in English
| MEDLINE
| ID: mdl-12454193
ABSTRACT
Dark-field microscopy of blood from healthy individuals revealed the existence of pleomorphic microorganisms. These bacteria exhibited limited growth and susceptibility to antibiotics and could be detected by fluorescent in situ hybridization and flow cytometry. They were further characterized by analysis of their 16S rRNA and gyrB genes.
Subject(s)
Bacteria/growth & development , Bacteria/isolation & purification , Blood/microbiology , Bacteria/genetics , Bacteria/ultrastructure , DNA Gyrase/genetics , DNA, Bacterial/analysis , DNA, Ribosomal/analysis , Flow Cytometry , Humans , In Situ Hybridization, Fluorescence , Microscopy, Electron , Molecular Sequence Data , RNA, Ribosomal, 16S/genetics
2.
J Clin Microbiol
; 40(12): 4792-6, 2002 Dec.
Article
in English
| MEDLINE
| ID: mdl-12454199
ABSTRACT
Campylobacter fetus subsp. fetus is a gram-negative, slender, spirally curved bacterial pathogen. It has been isolated from human blood, spinal fluid, and abscesses, but cellulitis associated with bacteremia is rare. We report its isolation from a blood culture of a human patient with cellulitis as well as difficulties encountered in determining the identity of the subspecies of C. fetus.