Inoculants of Arbuscular Mycorrhizal Fungi Influence Growth and Biomass of Terminalia arjuna under Amendment and Anamendment Entisol.

Entisol soil is hard and compact in nature, rendering it high in bulk density, which influences root penetration adversely and thereby poor plant growth. In this experiment, used seven treatments in different combination in normal soil, were used as growth media for the Terminalia arjuna seedling. T3 (60% entisol) found the best as it gave the highest biomass in the species regardless of arbuscular mycorrhizal fungi (AMF) treatment. AMF treatment enhanced the growth and biomass of plants significantly in all the given treatments. AMF colonization observed a maximum in tertiary roots. T1 (100% entisol soil) exhibited the highest degree of AMF colonization in tertiary roots, resulting in the highest mycorrhiza dependency of plants for this soil. The addition of normal soil to entisol soil was found to decrease the bulk density, resulting in increased root diameter, and T3 plants exhibited the highest biomass and AMF compatibility for T. arjuna species. The T. arjuna plant's growth and biomass responded positively to AMF in all types of treatments. The plant's growth and biomass were highest in the T3 treatment, which had a bulk density of 1.50 g/cm3. In this study, we combined the entisol with mycorrhizal inoculation of the nursery growing medium to promote plant growth and biomass, improve the plant's ability to hold water and absorb nutrients, and lower the entisol's bulk density. The T. arjuna (Roxb) plant responds very favorably to mycorrhiza inoculation in nursery conditions with the entisol growth medium.

Impact of integrated nutrient management on tomato yield under farmers field conditions.

Field trials were conducted in farmer's field of district Chandauli, Uttar Pradesh, India to assess the impact of integrated nutrient management (INM) on the performance of tomato crop during rabi (2008) and kharif (2009) season. Before conducting trials technological gap between actual and potential productivity were analyzed by interviewing growers to find out the major causes for low yield. Overall gap in use of fertilizers was recorded 64.90 % whereas overall mean gap in technology was 43.83%. On-farm experiments on INM were conducted by applying FYM (10t ha(-1)) + (NPK (150:80:60 kg ha(-1)) followed by dipping seedling roots in 1% Azotobacter solution for 15 min and foliar spray with 20 ppm ferrous ammonium sulphate after 30, 45 and 75 days of transplantation. The plant height, root length, number of primary branches, average fruit weight increased in INM plots as compared to farm practice. The increment in yield was found to be 28.84 and 33.86% during rabi and kharif season respectively. The maximum marketable yield obtained in INM plot during kharif and rabi seasons was 1025 q ha(-1) and 955 q ha(-1) respectively, whereas as farm practice yielded 740 q ha(-1) and 713 q ha(-1) during the same seasons. The percent loss from total production was recorded 8.5 % and 8.8 % in control plot and only 4.9 % and 5.7 % in INM plot during rabi and kharif seasons respectively. The higher fruit weight and lower incidence of disease and pest were observed in INM field in comparison to farm practice. The benefit cost ratio with INM treatment was recorded 4.25 and 4.23 in rabi and kharif season respectively against the benefit cost ratio of 2.98 and 2.82 in control plot during the same respective seasons.

Studies on mycorrhizal inoculation on dry matter yield and root colonization of some medicinal plants grown in stress and forest soils.

Five medicinal plants viz. Abelmoschatus moschatus Linn., Clitoria tematea L., Plumbagozeylanica L., Psorolea corylifolia L. and Withania sominifera L. were grown in a polypot experiment in five soils representing coal mine soil, coppermine soil, fly ash, skeletal soil and forest soil with and without mycorrhizal inoculations in a completely randomized block design. Dry matter yield and mycorrhizal root colonization of plants varied both in uninoculated and inoculated conditions. The forest soil rendered highest dry matter due to higher yield of A. moschatus, P. zeylanica and P corylifolia while fly ash showed lowest dry matter without any inoculants. P. cematea were best in coalmine soil and W. sominifera in copper mine soil without mycorrhizal inoculation. The mycorrhiza was found to enhance the dry matter yield. This contributed minimum 0.19% to maximum up to 422.0% in different soils as compared to uninoculated plants. The mycorrhizal dependency was noticed maximum in plants grown in fly ash followed by coal mine soil, copper mine soil, skeletal soil and forest soil. The mycorrhizal response was increased maximum in W. sominifera due to survival in fly ash after inoculation followed by P corylifolia and P cematea. Percent root colonization in inoculated plant was increased minimum of 1.10 fold to maximum of 12.0 folds in comparison to un-inoculated plants . The native mycorrhiza fungi were also observed to colonize 4.0 to 32.0% roots in plants understudy. This study suggests that mycorrhizal inoculation increased the dry matter yield of medicinal plants in all soils under study. It also helps in survival of W. sominifera in fly ash.

Study design and rationale of a mock phase I trial as an educational tool for clinical pharmacology residents.

OBJECTIVE: To invite comments and suggestions from the phase I trialists, the world over, on the design and rationale provided by us for conducting a mock phase I trial for training clinical pharmacology residents. METHODS: The trial will be conducted by clinical pharmacology residents of the Post Graduate Institute of Medical Education and Research. After a thorough evaluation of Preclinical toxicity data of a pharmaceutical product provided by a dummy pharmaceutical firm, a randomized, double-blind, placebo controlled, parallel group design with dose escalation of the product will be carried out. A single dose administration will be followed by monitoring the participants for 24 h and again at one week. After obtaining results of the previous group, the next higher dose will be administered. Evaluation of the learners will be done by two independent teachers who will mark them for actual conduct of the study and writing a report of the study. Subsequently, we will try to publish the results of the mock trial and invite further comments. CONCLUSIONS: This trial designed primarily for training purposes will be instrumental in equipping the residents with the expertise for conducting phase I trials.

Changing trends in the hospital management of unstable angina: a drug utilization analysis.

OBJECTIVE: The current study was designed to investigate drug utilization in the management of unstable angina in India and to examine the changing trends in the management of unstable angina over the past 4 years. METHODS: We conducted a prescription survey to examine the use of antianginal drugs in patients with unstable angina in a tertiary care Indian hospital. The use of concurrent medications such as antidiabetic, antihypertensive and lipid-lowering agents was also examined. This study results were compared with a similar study done in this institute 4 years earlier. RESULTS: A total of 159 consecutive prescriptions were evaluated. Aspirin (86%), nitroglycerin infusion (77%) and low-molecular weight heparins (93%) were the most frequently prescribed drugs. Enoxaparin accounted for 76% of the total LMWH use. One of the heparins was used by 92% of all patients, angiotensin-converting enzyme inhibitors (ACEIs) and beta-blockers by 70% and 67%, respectively. Lipid-lowering agents (57%), antidiabetic agents (16%) and antianxiety agents (33%), in addition to antianginals, were also frequently co-administered. Time trend analysis showed that the use of unfractionated heparin fell from 35% to 10% and the use of ACEIs and enoxaparin increased from 17% to 70% and from 51% to 71%, respectively. CONCLUSIONS: The study showed that unfractionated heparin is less frequently used in the treatment of unstable angina than in the past and that ACEIs are preferred to calcium channel blockers. Enoxaparin remains the most commonly used low-molecular weight heparin for this indication. A variety of low-molecular weight heparins are available for therapy but comparative clinical trials of efficacy and pharmacoeconomic studies comparing the various LMWHs still need to be carried out.

Utilization of parenteral anti-infective agents in the medical emergency unit of a tertiary care hospital: an observational study.

A pharmacy based prescription audit was undertaken in the medical emergency unit of a tertiary care hospital to determine the frequency of prescribing of parenteral anti-infective agents. During the study period, 885 patients were screened. The analysis was done for the number of parenteral anti-microbials in each prescription frequency of individual drug prescribe number and dose unit (DDD), frequency of age and sex, site of infection and daily cost incurred by the patient. It was found that 400 patients (45.2%) received parenteral anti-infective agents. Cephalosporins, aminoglycosides and metronidazole accounted for about 70% of total antimicrobial use. More than 50% of patients had culture sensitivity reports available. The mean (standard deviation, SD) daily cost of antibiotic was USD 3.8 (7.7), median; range 2 (0.1-85.7). Two anti-microbials per prescription were indicated in most of the patients (43.88). In conclusion we have provided an overview of parenteral anti-infective use in medical emergency, which may serve as a basis for intervention and improvement in prescribing pattern of parenteral anti-microbials.

Prehospital delay in patients hospitalized with acute myocardial infarction in the emergency unit of a North Indian tertiary care hospital.

BACKGROUND: Prompt treatment of patients presenting with acute myocardial infarction decreases the incidence of death from early arrhythmia, and maximizes the potential benefit of thrombolytic therapy. Prehospital delay has been identified as a major obstacle to the widespread use of thrombolytic therapy. The aim of the present study was to examine the extent of, and factors associated with, delay in seeking medical care (usually thrombolytic therapy) in patients with acute myocardial infarction. METHODS AND RESULTS: The study was conducted in patients visiting the medical emergency unit of the Nehru Hospital, Post Graduate Institute of Medical Education and Research, Chandigarh. A total of 104 patients diagnosed with acute myocardial infarction were interviewed using a pre-designed proforma. Pain-to-door, and door-to-drug times, were the main outcome measures. The corrected mean (SEM) and median (range) pain-to-door times were 8.5 (0.8) hours and 5.2 (0.5-24) hours, respectively. Out of 104 patients, 38 did not receive thrombolytic therapy. In those who did not receive thrombolytic therapy, prior therapy at local health centers, lack of knowledge of symptoms, and transportation problems were the main reasons for hospital delay. The mean (SEM) and median (range) of door-to-drug times were 1.2 (0.1) hours and 1 (0.2-3.5) hours, respectively.