ABSTRACT
Aquaculture plays an important role as one of the fastest-growing food-producing sectors in global food and nutritional security. Demand for animal protein in the form of fish has been increasing tremendously. Aquaculture faces many challenges to produce quality fish for the burgeoning world population. Cellular aquaculture can provide an alternative, climate-resilient food production system to produce quality fish. Potential applications of fish muscle cell lines in cellular aquaculture have raised the importance of developing and characterizing these cell lines. In vitro models, such as the mouse C2C12 cell line, have been extremely useful for expanding knowledge about molecular mechanisms of muscle growth and differentiation in mammals. Such studies are in an infancy stage in teleost due to the unavailability of equivalent permanent muscle cell lines, except a few fish muscle cell lines that have not yet been used for cellular aquaculture. The Prospect of cell-based aquaculture relies on the development of appropriate muscle cells, optimization of cell conditions, and mass production of cells in bioreactors. Hence, it is required to develop and characterize fish muscle cell lines along with their cryopreservation in cell line repositories and production of ideal mass cells in suitably designed bioreactors to overcome current cellular aquaculture challenges.
ABSTRACT
The aim of the present study was to evaluate the ability of fish collagen peptides (FCP) derived from the skin of great hammerhead shark (Sphyrna mokarran) in attenuating the high fat diet-alcohol induced hyperlipidemia. The oral supplementation of FCP in high fat diet-alcohol fed experimental rats confirmed the regulation of body weight to normal level. The FCP treated group revealed the efficient lipid lowering ability by enhancing the cholesterol metabolism. Western blot analysis of the lipid metabolic enzymes revealed that the oral-intake of FCP has down-regulated the expression levels of fatty acid synthase and 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGCR). Simultaneously, the expression levels of Lecithin-cholesterol acyltransferase (LCAT) in liver was up-regulated. Histopathology analysis of liver tissues demonstrated that the FCP treated group maintained normal liver parenchyma with moderate inflammatory infiltration, whereas the statin treated group developed centrilobular fibrosis, atrophy of hepatocytes and moderate inflammatory infiltration. Oral dietary supplementation of FCP enhanced the activity levels of both superoxide dismutase and catalase enzymes and, lowered the levels of lipid peroxidation in liver tissues. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13197-021-05118-0.
ABSTRACT
The effects of electron beam irradiation (0, 2.5, 5.0, 7.5 and 10 kGy) on the quality characteristics of vacuum packed headless vannamei stored under chilled condition (2 °C) was assessed. The total volatile base nitrogen, non protein nitrogen content, peroxide value and thiobarbituric acid reactive substances showed an increasing trend during storage. Texture analysis indicated that shrimp irradiated at above 2.5 kGy dose had a lower hardness than control. L* value showed a decreased trend during storage (51.87-13.89). Moreover, b* and a* value showed a gradual increase during storage. The total viable count was lower in the irradiated sample than control. A similar trend also observed for psychrophilic, pseudomonas and H2S former count. There is a significant (p < 0.05) reduction in Brochothrix thermosphacta and lactobacillus count in the irradiated sample. Based on the microbial and sensory analysis control had a shelf life up to 12th day. However, electron beam irradiated sample had extended shelf life of 15-23 days with respect to dose level.
ABSTRACT
Microencapsulation by spray drying is a well-accepted technique for fish oil stabilization. However, severe operational conditions during atomisation destabilise the emulsion, leading to capsule collapse and induction of auto-oxidation. Hence, use of food grade cross-linkers to strengthen the wall material and antioxidants to prevent lipid oxidation has been suggested. A promising option in this line is the use of bioactive peptides, which ensure oxidative stability through structural and chemical stabilisation. Present study attempted to compare the efficacy of yellowfin tuna red meat hydrolysate in protecting the core sardine oil, when used as wall and core polymer during encapsulation. Encapsulates were characterised based on morphological and physical parameters, as well as by in vitro digestibility studies. Their storage stability was also compared under accelerated (60 °C), chilled (4 °C) and ambient conditions (28 °C). Tuna protein hydrolysate exhibited significantly higher protective efficacy when used as core polymer rather than in the wall matrix of encapsulates.
