ABSTRACT
A transcriptomic analysis of cultured human uterine smooth muscle cells (hUtSMCs) was performed to examine gene expression profiles in smooth muscle in an environment containing the two major steroid hormones that regulate the human myometrium in physiological states associated with estrous, pregnancy, labor, and pathophysiological states such as leiomyoma and endometrial cancer. hUtSMCs were treated with progesterone (P4) and 17ß-estradiol (E2) individually and in combination, in the presence and absence of RU486 (mifepristone). Transcription of many genes was modulated in the presence of P4 or E2 alone, but almost six times more genes were transcriptionally modulated in the presence of the P4/E2 hormone combination. In total 796 annotated genes were significantly differentially expressed in the presence of both P4 and E2 relative to their expression in untreated cells. Functional withdrawal of P4 by addition of RU486 effectively reversed almost all transcriptional changes caused by P4/E2 treatment. Gene ontology analysis of differentially expressed genes revealed a strong association between P4/E2 treatment and downregulated expression of genes involved in cell communication, signal transduction, channel activity, inflammatory response, and differentiation. Upregulated processes included cell survival, gene transcription, steroid hormone biosynthesis, muscle development, insulin receptor signaling, and cell growth.
Subject(s)
Estradiol/pharmacology , Myocytes, Smooth Muscle/metabolism , Myometrium/cytology , Progesterone/pharmacology , Transcriptome/drug effects , Binding Sites , Cells, Cultured , Female , Gene Expression Profiling , Gene Expression Regulation/drug effects , Gene Ontology , Humans , Mifepristone/pharmacology , Myocytes, Smooth Muscle/drug effects , Real-Time Polymerase Chain Reaction , Reproducibility of Results , Transcription Factors/metabolism , Transcriptome/geneticsABSTRACT
Contractility of the myometrial smooth muscle cells during the estrous cycle and pregnancy is modulated by estrogen but the temporal expression of estrogen (relative to progesterone) and the type of contraction involved are distinctly different in pregnancy and estrous. This in vitro cell culture study investigated the global gene expression profile of human uterine smooth muscle cells (hUtSMCs) following 17ß-estradiol (E2) treatment. In response to E2 treatment 540 genes, many of which have not been previously described as estrogen responsive, were identified as significantly differentially expressed. These genes are involved in biological processes that include muscle contraction, cell migration and adhesion, apoptosis and phosphorylation. Evidence from this study suggests that 17ß-estradiol may have effects that are contrary to an overall contraction phenotype. The hUtSMC in vitro culture system is a useful model to investigate steroid effects on smooth muscle cells and may provide additional clues as to how smooth muscle cells behave in vivo.
Subject(s)
Estradiol/pharmacology , Gene Expression Regulation/drug effects , Myometrium/drug effects , ADP-ribosyl Cyclase 1/genetics , ADP-ribosyl Cyclase 1/metabolism , Cells, Cultured , Estrous Cycle , Female , Humans , Microarray Analysis , Muscle Contraction/drug effects , Myometrium/cytology , Myometrium/metabolism , Pregnancy , RNA, Messenger/genetics , RNA, Messenger/metabolism , TranscriptomeABSTRACT
Protein tyrosine phosphatases (PTPs) cooperate with protein tyrosine kinases to regulate signal transduction pathways. Genome-wide surveys cataloging protein tyrosine phosphatases in humans have recently been carried out. Here, we present a bioinformatics analysis of protein tyrosine phosphatases in the human genome to examine their domain architecture, alternative splicing and pseudogenes. We present evidence that alternative transcripts exist for 25 out of 35 PTPs analyzed. These alternative transcripts include novel exons; skipped exons as well as cryptic donor/acceptor splice sites. We discovered a novel isoform of PTPN18 based on analysis of expressed sequence tags (ESTs). The deletion of 4 exons in the catalytic domain of the novel isoform may alter the enzymatic activity toward its substrates. We were able to experimentally validate 2 of our novel isoform predictions through RT-PCR. Finally, a user-friendly web-based resource that consolidates the gene and protein annotations for all human protein tyrosine phosphatases has been developed and is freely available at http://ptpr.ibioinformatics.org.
Subject(s)
Genome, Human , Protein Tyrosine Phosphatases/metabolism , Alternative Splicing , Computational Biology , Exons , Expressed Sequence Tags , Humans , Isoenzymes/genetics , Isoenzymes/metabolism , Protein Tyrosine Phosphatases/genetics , Protein Tyrosine Phosphatases, Non-ReceptorABSTRACT
Plasma is one of the best studied compartments in the human body and serves as an ideal body fluid for the diagnosis of diseases. This report provides a detailed functional annotation of all the plasma proteins identified to date. In all, gene products encoded by 3778 distinct genes were annotated based on proteins previously published in the literature as plasma proteins and the identification of multiple peptides from proteins under HUPO's Plasma Proteome Project. Our analysis revealed that 51% of these genes encoded more than one protein isoform. All single nucleotide polymorphisms involving protein-coding regions were mapped onto the protein sequences. We found a number of examples of isoform-specific subcellular localization as well as tissue expression. This database is an attempt at comprehensive annotation of a complex subproteome and is available on the web at http://www.plasmaproteomedatabase.org.
Subject(s)
Blood Proteins/chemistry , Blood Proteins/genetics , Databases, Protein , Proteomics/methods , Amino Acid Motifs , Computational Biology/methods , Genome, Human , Humans , Mass Spectrometry , Peptides/chemistry , Polymorphism, Single Nucleotide , Protein Isoforms , Protein Structure, Tertiary , Time FactorsSubject(s)
Chromosomes, Human, X/genetics , Alternative Splicing , Amino Acid Sequence , Base Sequence , Chromosome Mapping , Computational Biology , DNA, Complementary/genetics , Databases, Genetic , Databases, Protein , Exons , Humans , Molecular Sequence Data , Open Reading Frames/physiology , Sequence AlignmentABSTRACT
The rapid pace at which genomic and proteomic data is being generated necessitates the development of tools and resources for managing data that allow integration of information from disparate sources. The Human Protein Reference Database (http://www.hprd.org) is a web-based resource based on open source technologies for protein information about several aspects of human proteins including protein-protein interactions, post-translational modifications, enzyme-substrate relationships and disease associations. This information was derived manually by a critical reading of the published literature by expert biologists and through bioinformatics analyses of the protein sequence. This database will assist in biomedical discoveries by serving as a resource of genomic and proteomic information and providing an integrated view of sequence, structure, function and protein networks in health and disease.
Subject(s)
Databases, Protein , Proteins/metabolism , Proteomics , Computational Biology , Disease , Genomics , Humans , Information Storage and Retrieval , Internet , Protein Binding , Protein Processing, Post-Translational , Proteins/chemistry , Proteins/genetics , Proteome/chemistry , Proteome/genetics , Proteome/metabolism , Substrate Specificity , Vocabulary, ControlledABSTRACT
Human Protein Reference Database (HPRD) is an object database that integrates a wealth of information relevant to the function of human proteins in health and disease. Data pertaining to thousands of protein-protein interactions, posttranslational modifications, enzyme/substrate relationships, disease associations, tissue expression, and subcellular localization were extracted from the literature for a nonredundant set of 2750 human proteins. Almost all the information was obtained manually by biologists who read and interpreted >300,000 published articles during the annotation process. This database, which has an intuitive query interface allowing easy access to all the features of proteins, was built by using open source technologies and will be freely available at http://www.hprd.org to the academic community. This unified bioinformatics platform will be useful in cataloging and mining the large number of proteomic interactions and alterations that will be discovered in the postgenomic era.
