Rapid detection of equine infectious anaemia virus nucleic acid by insulated isothermal RT-PCR assay to aid diagnosis under field conditions.

BACKGROUND: Control of equine infectious anaemia (EIA) currently depends on serological diagnosis of infected equids. However, recently infected equids may not produce detectable anti-EIAV antibodies up to 157 days post infection and so present a high transmission risk. Therefore, direct nucleic acid detection methods are urgently needed to improve EIAV surveillance and management programs in counties where the disease is endemic. OBJECTIVES: To evaluate a field-deployable, reverse transcription-insulated isothermal PCR (RT-iiPCR) assay targeting the conserved 5' untranslated region (5' UTR)/exon 1 of the tat gene of EIAV. STUDY DESIGN: The analytical and clinical performance of the newly developed EIAV RT-iiPCR was evaluated by comparison with a EIAV real-time RT-PCR (RT-qPCR) along with the AGID test. METHODS: Analytical sensitivity was determined using in vitro transcribed RNA containing the target area of the 5' UTR/tat gene and samples from two EIAV-positive horses. Specificity was verified using nine common equine viruses. Clinical performance was evaluated by comparison with EIAV RT-qPCR and AGID using samples derived from 196 inapparent EIAV carrier horses. RESULTS: EIAV RT-iiPCR did not react with other commonly encountered equine viruses and had equivalent sensitivity (95% detection limit of eight genome equivalents), with a concordance of 95.41% to conventional EIAV RT-qPCR. However, the RT-qPCR and RT-iiPCR had sensitivities of 43.75 and 50.00%, respectively, when compared to the AGID test. MAIN LIMITATIONS: Low viral loads commonly encountered in inapparent EIAV carriers may limit the diagnostic sensitivity of RT-PCR-based tests. CONCLUSIONS: Although EIAV RT-iiPCR is not sufficiently sensitive to replace the current AGID test, it can augment control efforts by identifying recently exposed or "serologically silent" equids, particularly as the latter often represent a significant transmission risk because of high viral loads. Furthermore, the relatively low cost and field-deployable design enable utilisation of EIAV RT-iiPCR even in remote regions.

Smoking cessation in Taiwanese male smokers with coronary artery disease: influencing factors and policy implications.

AIM: To investigate factors affecting smoking cessation in male smokers with coronary artery disease. DESIGN: A descriptive, correlation, cross-sectional study was conducted. METHODS: Data were collected using a questionnaire constructed by a literature review of research on smoking cessation in male smokers including demographics and smoking background, as well as psychological, interpersonal and environmental factors. A total of 130 male patients with coronary artery disease were recruited from the cardiac clinic at a regional hospital in Taiwan from August to December 2008. The response rate was 93% (n = 121). Descriptive statistics, chi-square, t-tests and logistic regression analysis were conducted. RESULTS: During the survey, 64.5% of the respondents reported that they had stopped smoking after a coronary event. Five factors were significantly associated with smoking cessation after diagnosis of coronary artery disease: age, the severity of heart diagnoses, antismoking norms (perceived that smoking was against the social norms), nicotine dependence level, and contrary views of smoking (perceived negative expectancy of smoking). Multivariate analysis revealed antismoking norms to be the most important predictor (AOR = 4.27; P < .05) after adjusting age. CONCLUSION: The study highlights the need to develop smoking cessation interventions that specifically counsel patients with coronary artery disease about these information, such as the disease risk and development, health consequences of smoking, and dependence therapy. Additionally, government support for tobacco control programmes in hospitals and elsewhere is designed to have a beneficial effect on patients' smoking behaviours primarily by increasing antismoking norms.

Angular distribution of polarized photon-pairs in a scattering medium with a Zeeman laser scanning confocal microscope.

A novel confocal microscope designed for use with turbid media is proposed. We use a Zeeman laser as the light source. Based on the properties of two-frequency polarized photon-pairs and the common-path feature of polarized photon-pairs with heterodyne detection employed in the proposed confocal microscope, three gatings (spatial filtering gating, polarization gating and spatial coherence gating) are thus simultaneously incorporated in the microscope. Experimental results for the angular distribution of polarized photon-pairs in a scattering medium indicate that polarization gating and spatial coherence gating preclude the detection of multiply scattered photons, whereas the pinhole selects the least scattered photon-pairs. Thus, better performance for axial resolution than can be obtained with a conventional confocal microscope is demonstrated experimentally. In addition, the proposed microscope is able to either look deeper into a turbid medium or work with a denser medium; furthermore, the axial resolution is improved.

Reliability of landmark identification in cephalometric radiography acquired by a storage phosphor imaging system.

OBJECTIVES: To compare the cephalometric landmark identification on softcopy and hardcopy of direct digital cephalography acquired by a storage-phosphor (SP) imaging system. METHODS: Ten digital cephalograms and their conventional counterpart, hardcopy on a transparent blue film, were obtained by a SP imaging system and a dye sublimation printer. Twelve orthodontic residents identified 19 cephalometric landmarks on monitor-displayed SP digital images with computer-aided method and on their hardcopies with conventional method. The x- and y-coordinates for each landmark, indicating the horizontal and vertical positions, were analysed to assess the reliability of landmark identification and evaluate the concordance of the landmark locations in softcopy and hardcopy of SP digital cephalometric radiography. RESULTS: For each of the 19 landmarks, the location differences as well as the horizontal and vertical components were statistically significant between SP digital cephalometric radiography and its hardcopy. Smaller interobserver errors on SP digital images than those on their hardcopies were noted for all the landmarks, except point Go in vertical direction. The scatter-plots demonstrate the characteristic distribution of the interobserver error in both horizontal and vertical directions. Generally, the dispersion of interobserver error on SP digital cephalometric radiography is less than that on its hardcopy with conventional method. CONCLUSIONS: The SP digital cephalometric radiography could yield better or comparable level of performance in landmark identification as its hardcopy, except point Go in vertical direction.

Thin-plate spline (TPS) graphical analysis of the mandible on cephalometric radiographs.

We describe two cases of Class III malocclusion with and without orthodontic treatment. A thin-plate spline (TPS) analysis of lateral cephalometric radiographs was used to visualize transformations of the mandible. The actual sites of mandibular skeletal change are not detectable with conventional cephalometric analysis. These case analyses indicate that specific patterns of mandibular transformation are associated with Class III malocclusion with or without orthopaedic therapy, and visualization of these deformations is feasible using TPS graphical analysis.

Correlation of cervical vertebra maturation with hand-wrist maturation in children.

The purpose of this study was to evaluate the reliability of cervical vertebra maturation as an indicator of skeletal age during the circumpubertal period. This was determined by correlating cervical vertebra maturation to hand-wrist maturation. The vertebral skeletal age was assessed using lateral cephalometric radiographs according to maturity indicators modified from Lamparski. The hand-wrist skeletal age was evaluated in radiographs with the system developed by Fishman. The sample consisted of 503 subjects (244 boys and 259 girls), aged 8 through 18 years. The Spearman rank correlation coefficients and Wilcoxon sign rank test showed that a statistically significant relationship existed between the two assessments. Both the intra- and inter-judge tests of reliability displayed no significant differences. The results of this study indicate that skeletal age assessment made from the maturational changes of cervical vertebrae were reliable, reproducible and valid.

Product ion studies of diastereomeric benzo[ghi]fluoranthene tetraols by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and post-source decay.

The product ion formation characteristics of the four diastereomeric tetrahydroxy benzo[ghi]fluoranthene compounds formed by hydrolysis of the syn and anti diastereomers of trans-3,4-dihydroxy-5,5a-epoxy-3,4,5,5a-tetrahydrobenzo[ghi]fluoranthene are studied using matrix-assisted laser desorption/ionization and post-source decay (PSD) to determine a correlation between the fragmentation characteristics of these tetraols and the structures of the diol-epoxide diastereomers from which they are hydrolyzed. The tetraols formed by the trans ring opening of the diol epoxides during hydrolysis yield product ion spectra specific for the syn and anti configurations of their precursor diol epoxides. All four diastereomeric tetraols form product ions by the losses of one and/or two water molecules in varying proportions when lithium-cationized molecule ions (m/z 301) are selected for PSD product ion analysis. The differences in the PSD spectra of these four Li+-cationized molecules are rationalized in terms of a water loss mechanism that involves the 1,2 elimination of a hydrogen atom and hydroxyl group that are cis with respect to each other on adjacent carbons.

Group learning factors in a problem-based course in oral radiology.

OBJECTIVES: To assess the development of group dynamics in students who participated in a problem-based learning (PBL) course in oral radiology and to evaluate the influence of the facilitators' experience. METHODS: At the beginning and end of the course, students in three consecutive years (1997, 1998 and 1999) responded to a group learning factor questionnaire designed to measure group dynamics (engagement, interpersonal relationships, lack of commitment and disruptive behaviour, interpersonal learning and self-presentation). The answers were analysed with paired t-test and the effects of the facilitator's seniority, expertise in oral radiology, experience of PBL and single or rotating facilitators assessed. RESULTS: Most groups in the three years reported above average group dynamic scores. There were significant changes in group dynamics. In the 1997 class, interpersonal relationships improved (P=0.014). In the 1998 class, interpersonal learning (P=0.021) and engagement (P=0.009) increased significantly, while disruptive behaviour decreased (P=0.031). In the 1999 class, lack of commitment and disruptive behaviour decreased (P=0.014). Groups led by a junior faculty member with no previous experience of PBL show less commitment and more disruptive behaviour. CONCLUSIONS: More commitment and less disruptive behaviour and improved engagement, interpersonal relationships and self-presentation were found in a PBL course in oral radiology. Facilitators should have prior exposure to PBL to avoid disruptive behaviour among the students.

Effects of Nd:YAG laser irradiation on cultured human gingival fibroblasts.

BACKGROUND AND OBJECTIVE: The Nd:YAG laser has been proposed to apply in minor soft tissue surgery, including various periodontal procedures. However, little information is available regarding the direct effect of Nd:YAG laser on gingival fibroblasts, which play an important role in the early healing processes of periodontal repair. STUDY DESIGN/MATERIALS AND METHODS: Nd:YAG laser irradiation was performed in pulsed mode on human gingival fibroblasts, which was derived from healthy human gingiva by an explant method. The size of laser diode was 400 microm in diameter. The parameters in laser delivery were pulse energy (50-150 mJ), power output (1.0-3.0 W), pulse rate (10-30 pps), and fixed duration of irradiation (10 seconds). The cell cultures were analysed by cytomorphologic examination under phase-contrast and scanning electron microscope. The vitality was also examined with the help of MTT staining. RESULTS: The area of laser damage on cell culture was circular in shape, with diameter beyond the size of laser diode. By scanning electron microscopy, we observed the cellular damage of cultured gingival fibroblasts induced by Nd:YAG laser irradiation, comparable with the progressive increased power settings. The cytomorphologic changes ranged from disappearance of cellular boundary, loss of identifiable cellular nucleus, and finally cell contraction and vacuolization. Significant decrease in cellular vitality (14% approximately 44%) after laser treatment with irradiation distance of nearly contact was noted. However, 2 mm defocusing irradiation with the same power settings did not significantly decrease cellular vitality. CONCLUSION: Our study demonstrated the cell damaging effects of Nd:YAG laser, ranging from degeneratively cytomorphologic change to cell death, on the cultured human gingival fibroblasts. It provided the dentist a chance to understand the potential hazard of laser application in periodontal treatment. If the energy output is enough for the clinical purposes, Nd:YAG laser with lower pulse energy and corresponding pulse rate should be selected to minimize the damage on adjacent soft tissue.

The NLM Indexing Initiative.

The objective of NLM's Indexing Initiative (IND) is to investigate methods whereby automated indexing methods partially or completely substitute for current indexing practices. The project will be considered a success if methods can be designed and implemented that result in retrieval performance that is equal to or better than the retrieval performance of systems based principally on humanly assigned index terms. We describe the current state of the project and discuss our plans for the future.

Comparison of landmark identification in traditional versus computer-aided digital cephalometry.

The aim of this study was to assess landmark identification on digital images in comparison with those obtained from original radiographs. Ten cephalometric radiographs were selected randomly. Seven orthodontic residents identified 19 cephalometric landmarks on both the original radiographs and the digital images. To assess the concordance between landmarks identified on the original radiographs and on their digital counterparts, the x, y coordinates for each landmark in the 2 modalities were transformed with the identical references. The placement differences for 19 landmarks between 2 methods were calculated and their components in horizontal and vertical directions were analyzed respectively. Multivariate analysis of variance showed that the "cephalometric radiograph" and "landmark" variation had greater influence than that from "method" (landmark identification on digital / original radiograph). It was also noted that the differences of landmark identification between original radiographs and their digital counterparts were statistically significant. The landmarks with significant differences of horizontal component on the x-axis were Me, Gn, ANS, PNS, LIA. The differences were generally under 1 mm with the exception of Or, Me, PNS, LIA. The landmarks with significant differences of vertical component on the y-axis were Po, Or, Gn. The inter-observer error for each landmark in digital images was generally larger than that in the original radiographs. However, statistically significant differences of inter-observer errors between 2 modalities were only found for 4 of the 19 landmarks. These 4 landmarks, Po, Ar, ANS, and UM, should be scrutinized more carefully during potential applications of digital cephalometry.

The response of cAMP and DNA synthesis in rat osteosarcoma cells to mechanically deformed culture dishes.

The purpose of this research was to document the biochemical response of rat osteosarcoma cells (ROS 17/2.8) to mechanical stress in vitro. The influence of mechanical stress on ROS 17/2.8 cells was studied using a stress application device. Briefly, the device was fabricated by bonding an orthodontic expansion screw outside the bottom of a plastic culture dish with self-curing acrylic resin. Irreversible deformation of the culture dish was produced by activating an expansion screw. The resulting mechanical stress was transferred to the cells which attached to the culture dish. The response in terms of cyclic adenosine monophosphate (cAMP) of ROS 17/2.8 cells to mechanical stress was measured using a competitive protein-binding method. The effect of mechanical stress on cellular growth was assessed through the incorporation of 3H-thymidine after different periods of mechanical stress application. The results revealed that mechanical stress could increase the production of cAMP in ROS 17/2.8 cells at an early phase after stress stimulation. This change in the cAMP level was dependent on the duration of stress application, and the maximal response occurred when the mechanical stress was applied for one hour. Although the cellular incorporation of 3H-thymidine decreased 40-60% in ROS 17/2.8 cells subjected to mechanical stress for 1 hour, this reaction recovered from the inhibition effect to 80-85% of the baseline when the mechanical stress lasted for 24 hours. The observations in this study indicate that mechanical stress stimulates the production of cAMP and inhibits the 3H-thymidine incorporation of ROS 17/2.8 cells at an early phase of stress application.

Beyond synonymy: exploiting the UMLS semantics in mapping vocabularies.

The Unified Medical Language System (UMLS) contains semantic information about terms from various sources, each concept can be understood and located by its relationships to other concepts: this is a result of the organizing principle of semantic locality. We describe a method in which the semantic relationships between concepts are used to map concepts from different vocabularies in the UMLS. Applied to mapping concepts to MeSH, this method is able to map 50 to 65% of the non-MeSH concepts to MeSH. A manual review of the mapping shows a relevance rate of 61%. Causes of failure include a lack of consistently represented relationships in the UMLS, and some inconsistencies in the categorization of the concepts. The limits of this method are discussed, as well as possible adaptations for other uses.

Observation of bolus position with standardized test foods and fluoroscopic technique.

Sugar and agar balls containing barium were made for the fluoroscopic observation of the bolus position during habitual side chewing in 13 adult males (aged from 20 to 28 years) who had a complete dentition and healthy masticatory system. The test foods had constant size, shape, weight and known hardness, i.e. type 1, 20 kg; type 2, 40 kg; type 3, 1.0 kg and type 4, 0.5 kg. These four types of test foods were chewed randomly in a fluoroscopic room. The jaw separation distance and the bolus position during first bite and crushing bite were observed. The influence of the chewing order of four types of test foods on the bolus position was also recorded. It was found that both hard and soft test foods were most often crushed at the first molar area, and the separation distance during the crushing bite was only slightly wider than the diameter of the food. The first bite jaw separation distance for hard food chewing was significantly wider than soft food chewing and the later in the chewing order the wider the separation distance. The effect of chewing order was not significant on the position and jaw separation distance of the crushing bite.

Exon 8 mutation of p53 gene associated with nodal metastasis in non-small-cell lung cancer.

The epidemiologic characteristics of lung cancer in Taiwan differ from those in other parts of the world in low male-to-female ratio, the high percentage of adenocarcinoma, and the relatively high percentage of nonsmokers who are victims. To investigate possible correlation between p53 gene alteration and the unique characteristics of lung cancer here, p53 gene status of 36 patients with primary, resected non-small-cell lung cancer (NSCLC) was studied by directly sequencing the cDNA of the p53 gene, then acquiring clinical and pathologic data to correlate p53 gene status with clinical parameters and pathologic staging. Missense mutations were present in 42% (15 of 36) of patients with NSCLC, including 42% (10 of 24) with adenocarcinomas, and 45% (five of 11) with squamous cell carcinomas. The frequency of p53 mutation was 50% in smokers and 29% in nonsmokers (p = 0.355). The mutation occurred most frequently in exon 8 (56%), and G:C to A:T transitions in non-CpG or CpG sites were the most commonly observed base changes (56%). These findings differ from the high prevalence of G to T transversion found in previous reports. The frequency of metastasis in hilar and mediastinal lymph nodes was significantly higher in tumors with p53 mutations. The association with nodal stage was strong for mutations within exon 8, but it was less apparent for mutations in other exons probably because of the small number. This study suggests that p53 gene missense is common in NSCLC in Taiwan, but smoking is probably not the sole contributing factor. More interestingly, p53 gene mutations, especially those in exon 8, may be associated with regional nodal metastasis.

[Dento-facial structural characteristics in Angle Class II malocclusion associated with abnormal facial divergency].

Lateral cephalometric radiographs of 60 adult patients with Angle class 11 malocclusion associated with abnormal facial divergency were collected from the Orthodontic Department of the National Taiwan University Hospital. They were divided into a hyperdivergent group (35 cases) and a hypodivergent group (25 case), according to mandibular plane angle (SN-MP). The 19 landmarks on each cephalometric tracing were digitized into a computer, then computer-aided cephalometric analysis was performed to calculate the 17 skeletal measurements and 13 dentoalveolar measurements. The dento-facial structural characteristics of the hyperdivergent and hypodivergent groups were compared. It was found that the subjects of the hyperdivergent group revealed a greater tendency of divergency in the anterior cranial base plane, Frank-fort horizontal plane, palatal plane, occlusal plane, and mandibular plane. Hyperdivergent facial type, supposedly indicating an open bite or a tendency toward an open bite, has a longer lower anterior facial height, shorter posterior facial height, longer upper anterior and posterior dental height. While, the majority of dentofacial characteristics of the hypodivergent facial type observed in is study were directly opposite to those of the hyperdivergent facial type. The relationships of incisor overbite depth and other skeletal and dentoalveolar parameters were illustrated by Pearson's correlation coefficient and stepwise multiple regression analysis by means of the SPSS/PC statistic program. With the incisor overbite depth as the dependent variable, the independent variables included on the regression analysis were the 10 items of skeletal and dentoalveolar parameters. The compared parameters showed a statistically significant correlation with the incisor overbite depth (P < 0.001). By the stepwise method, the variables included on the regression equation were (1) N-Go-Gn, (2) A-Gn-Ar, (3) N-Ans/ans-Me, and (4) U1L1. The value of R square (R2) in the regression analysis was 0.543. It demonstrated that only a 54.3% variation in incisor overbite depth can be explained by variations in those skeletal and dentoalveolar variables.

A cross-sectional study on the skeletal development of the hand and wrist from preadolescence to early adulthood among Chinese in Taiwan.

In order to study the skeletal development of the hand-wrist among Chinese in Taiwan, a total of 1,679 hand-wrist radiographs were collected from the files in the Division of Orthodontics, School of Dentistry, National Taiwan University and the Orthodontic Clinic of the National Taiwan University Hospital. The ages of the subjects ranged from 6.3 years to 22.7 years. The nine commonly used ossification events, PP2 = ,MP3 = ,S, MP3cap, DP3u, MP3u, Riu, Rau and Rcu, were used in this study. It was found that the sequence of the nine skeletal maturation stages did not differ between males and females and was generally in agreement with similar studies among other ethnic groups. A statistically significant difference did exist between the males and females in the mean ages of onset of the skeletal maturation stages. All of the skeletal stages were more advanced in the females than in the males. The mean ages of the nine skeletal maturation stages obtained in this study could be used as a reference for daily pedodontic and orthodontic practice as well as for related studies. Although there was no intention of making a statistical comparison of the data for the Chinese with those of other ethnic groups, some diagrammatic illustrations for comparison were made.

[The vector analysis of the anteroposterior relationship among the apical bases and the incisors].

Using the lateral cephalometric radiographs, the points A, B, maxillary incisor tip (U) and mandibular incisor tip (L) were projected onto the Frankfort horizontal plane and the projected points were defined as a, b, u and 1, respectively. According to the principle of the vector analysis, the vector formula ab - ul = au - bl can always stand irrespective of how the locations of the points a, b, u and 1 vary. The vectors ab, ul, au and bl represent the anteroposterior linear relationship between the upper jaw and the lower jaw, the upper incisor and the lower incisor (overjet), the upper jaw and the upper incisor, and the lower jaw and the lower incisor, respectively with reference to the FH plane. The lateral cephalometric radiographs taken from a total of 863 class I subjects, including 422 males and 441 females, were submitted for the study. The four vector values were obtained from the tracing of each radiograph and the mean values were computed according to sex and age. If a positive value was given to the vector ab, it denoted that point "a" was anterior to point "b", while a negative value denoted that "a" was posterior to "b". Both sexes of the subjects were divided according to their ages into junior primary (JP, 6-8 years), senior primary (SP, 9-11 years), junior high (JH, 12-14 years), and senior high (SH, 15-18 years) groups. No substantial or significant mean difference of each vector between the males and the females in each age group was noted. The mean values of the vector ab were 7.4, 6.8, 6.1 and 4.7 mm in JP, SP, JH and SH groups, respectively with the male and the female data pooled together. This indicates that there was a gradual catching up tendency of mandibular versus maxillary growth. The mean values of the vector ul were 1.4, 2.8, 2.9 and 2.8 mm in each successive age group, respectively showing that the overjet was rather constant with age except in the youngest group. The corresponding mean values were -0.4, -3.9, -5.2 and -6.1 mm for the vector au and -6.3, -8.0, -8.4 and -8.0 mm for the vector bl in JP, SP, JH and SH groups, respectively. The data indicate that both upper and lower incisors were relatively more upright in the youngest group when compared with the other age groups. In the remaining groups the upper incisors exhibited a tendency of gradual labial tipping with age, whereas the lower incisors kept stable in the procumbency.(ABSTRACT TRUNCATED AT 400 WORDS)