Insights into the influence of polystyrene microplastics on the bio-degradation behavior of tetrabromobisphenol A in soil.

Soil contamination by emerging pollutants tetrabromobisphenol A (TBBPA) and microplastics has become a global environmental issue in recent years. However, little is known about the effect of microplastics on degradation of TBBPA in soil, especially aged microplastics. In this study, the effect of aged polystyrene (PS) microplastics on the degradation of TBBPA in soil and the mechanisms were investigated. The results suggested that the aged microplastics exhibited a stronger inhibitory effect on the degradation of TBBPA in soil than the pristine microplastics, and the degradation efficiency of TBBPA decreased by 21.57% at the aged microplastic content of 1%. This might be related to the higher TBBPA adsorption capacity of aged microplastics compared to pristine microplastics. Aged microplastics strongly altered TBBPA-contaminated soil properties, reduced oxidoreductase activity and affected microbial community composition. The decrease in soil oxidoreductase activity and relative abundance of functional microorganisms (e.g., Bacillus, Pseudarthrobacter and Sphingomonas) caused by aged microplastics interfered with metabolic pathways of TBBPA. This study indicated the importance the risk assessment and soil remediation for TBBPA-contaminated soil with aged microplastics.

Effect of high corn straw loads on short-chain fatty acid production in semi-continuous rumen reactor.

Ruminal microorganisms can efficiently hydrolyze biomass waste for short-chain fatty acid (SCFA) production. However, the continuous SCFA production by ruminal microorganisms at high loads is unclear. In this study, the effectiveness of a rumen semi-continuous reactor at high load for SCFA production was explored. Results showed that SCFA concentration reached 13.3 g/L at 8 % (w/v) corn straw load. The higher the corn straw load, the lower the volatile solid removal. Rumen microbial community composition changed significantly with increasing corn straw load. A significant decrease in bacterial diversity and abundance was observed at 8 % corn straw load. Some core genera such as Prevotella, Saccharofermentans, and Ruminococcus significantly increased. As corn straw loads increased, the expression of functional genes related to hydrolysis and acidogenesis gradually increased. Thus, the 8.0 % load is suitable for SCFA production. These findings provide new insights into high load fermentation of ruminal microorganisms.

Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review.

The rumen of ruminants is a natural anaerobic fermentation system that efficiently degrades lignocellulosic biomass and mainly depends on synergistic interactions between multiple microbes and their secreted enzymes. Ruminal microbes have been employed as biomass waste converters and are receiving increasing attention because of their degradation performance. To explore the application of ruminal microbes and their secreted enzymes in biomass waste, a comprehensive understanding of these processes is required. Based on the degradation capacity and mechanism of ruminal microbes and their secreted lignocellulose enzymes, this review concentrates on elucidating the main enzymatic strategies that ruminal microbes use for lignocellulose degradation, focusing mainly on polysaccharide metabolism-related gene loci and cellulosomes. Hydrolysis, acidification, methanogenesis, interspecific H2 transfer, and urea cycling in ruminal metabolism are also discussed. Finally, we review the research progress on the conversion of biomass waste into biofuels (bioethanol, biohydrogen, and biomethane) and value-added chemicals (organic acids) by ruminal microbes. This review aims to provide new ideas and methods for ruminal microbe and enzyme applications, biomass waste conversion, and global energy shortage alleviation.

Bioconversion of volatile fatty acids from organic wastes to produce high-value products by photosynthetic bacteria: A review.

Anaerobic fermentation of organic waste to produce volatile fatty acids (VFAs) production is a relatively mature technology. VFAs can be used as a cheap and readily available carbon source by photosynthetic bacteria (PSB) to produce high value-added products, which are widely used in various applications. To better enhance the VFAs obtained from organic wastes for PSB to produce high value-added products, a comprehensive review is needed, which is currently not available. This review systematically summarizes the current status of microbial proteins, H2, poly-ß-hydroxybutyrate (PHB), coenzyme Q10 (CoQ10), and 5-aminolevulinic acid (ALA) production by PSB utilizing VFAs as a carbon resource. Meanwhile, the metabolic pathways involved in the H2, PHB, CoQ10, and 5-ALA production by PSB were deeply explored. In addition, a systematic resource utilization pathway for PSB utilizing VFAs from anaerobic fermentation of organic wastes to produce high value-added products was proposed. Finally, the current challenges and priorities for future research were presented, such as the screening of efficient PSB strains, conducting large-scale experiments, high-value product separation, recovery, and purification, and the mining of metabolic pathways for the VFA utilization to generate high value-added products by PSB.

Response of rumen microorganisms to pH during anaerobic hydrolysis and acidogenesis of lignocellulose biomass.

Rumen microorganisms can efficiently degrade lignocellulosic wastes to produce volatile fatty acids (VFAs). pH is a key factor in controlling the type and yield of VFAs by affecting the microorganisms involved in rumen fermentation. However, the effects of different pH on rumen microbial diversity, communities, and mechanisms are unclear. In this study, the hydrolysis and acidogenesis of corn straw and diversity, communities, and mechanisms of rumen microorganisms were explored at different initial pHs. Results showed that the highest hemicellulose, cellulose, and lignin degradation efficiency of corn straw was 55.2 %, 38.3 %, and 7.01 %, respectively, and VFA concentration was 10.2 g/L at pH 7.0. Low pH decreased the bacterial diversity and increased the fungal diversity. Rumen bacteria and fungi had different responses to initial pHs, and the community structure of bacteria and fungi had obviously differences at the genus level. The core genera Succiniclasticum, Treponema, and Neocallimastix relative abundance at initial pH 7.0 samples were significantly higher than that at lower initial pHs, reaching 6.01 %, 1.61 %, and 5.35 %, respectively. The bacterial network was more complex than that of fungi. pH, acetic acid, and propionic acid were the main factors influencing the bacterial and fungal community structure. Low pH inhibited the expression of functional genes related to hydrolysis and acidogenesis, explaining the lower hydrolysis and acidogenesis efficiency. These findings will provide a better understanding for rumen fermentation to produce VFAs.

Adsorption behaviors and bioavailability of tetrabromobisphenol A in the presence of polystyrene microplastic in soil: Effect of microplastics aging.

Microplastics, a kind of emerging pollutant, have become a global environmental research hotspot in recent years due to its wide distribution in soil and its impact on soil ecosystems. However, little information is available on the interactions between microplastics and organic contaminants in soil, especially after microplastic aging. The impact of polystyrene (PS) microplastic aging on the sorption of tetrabromobisphenol A (TBBPA) in soil and the desorption characteristics of TBBPA-loaded microplastics in different environments were studied. The results showed a significant increase of 76.3% in adsorption capacity of TBBPA onto PS microplastics after aging for 96 h. Based on the results of characterization analysis and density functional theory (DFT) calculation, the mechanisms of TBBPA adsorption changed mainly from hydrophobic and π-π interactions on pristine PS microplastics to hydrogen bond and π-π interactions on aged PS microplastics. The presence of PS microplastics increased the TBBPA sorption capacity onto soil-PS microplastics system and significantly altered the distribution of TBBPA on soil particles and PS microplastics. The high TBBPA desorption over 50% from aged PS microplastics in simulated earthworm gut environment suggested that TBBPA contamination combined with PS microplastics might pose a higher risk to macroinvertebrates in soil. Overall, these findings contribute to the understanding of impact of PS microplastic aging in soil on the environmental behaviors of TBBPA, and provide valuable reference for evaluating the potential risk posed by the co-existence of microplastics with organic contaminants in soil ecosystems.

Metagenomic analysis reveals the efficient digestion mechanism of corn stover in Angus bull rumen: Microbial community succession, CAZyme composition and functional gene expression.

Ruminant rumen is a biological fermentation system that can efficiently degrade lignocellulosic biomass. The knowledge about mechanisms of efficient lignocellulose degradation with rumen microorganisms is still limited. In this study, composition and succession of bacteria and fungi, carbohydrate-active enzymes (CAZymes), and functional genes involved in hydrolysis and acidogenesis were revealed during fermentation in Angus bull rumen via metagenomic sequencing. Results showed that degradation efficiency of hemicellulose and cellulose reached 61.2% and 50.4% at 72 h fermentation, respectively. Main bacterial genera were composed of Prevotella, Butyrivibrio, Ruminococcus, Eubacterium, and Fibrobacter, and main fungal genera were composed of Piromyces, Neocallimastix, Anaeromyces, Aspergillus, and Orpinomyces. Principal coordinates analysis indicated that community structure of bacteria and fungi dynamically changed during 72 h fermentation. Bacterial networks with higher complexity had stronger stability than fungal networks. Most CAZyme families showed a significant decrease trend after 48 h fermentation. Functional genes related to hydrolysis decreased at 72 h, while functional genes involved in acidogenesis did not change significantly. These findings provide a in-depth understanding of mechanisms of lignocellulose degradation in Angus bull rumen, and may guide the construction and enrichment of rumen microorganisms in anaerobic fermentation of waste biomass.

Toxicological effects, environmental behaviors and remediation technologies of herbicide atrazine in soil and sediment: A comprehensive review.

Atrazine has become one of the most popular applied triazine herbicides in the world due to its high herbicidal efficiency and low price. With its large-dosage and long-term use on a global scale, atrazine can cause widespread and persistent contamination of soil and sediment. This review systematically evaluates the toxicological effects, environmental risks, environmental behaviors (adsorption, transport and transformation, and bioaccumulation) of atrazine, and the remediation technologies of atrazine-contaminated soil and sediment. For the adsorption behavior of atrazine on soil and sediment, the organic matter content plays an extremely important role in the adsorption process. Various models and equations such as the multi-media fugacity model and solute transport model are used to analyze the migration and transformation process of atrazine in soil and sediment. It is worth noting that certain transformation products of atrazine in the environment even have stronger toxicity and mobility than its parent. Among various remediation technologies, the combination of microbial remediation and phytoremediation for atrazine-contaminated soil and sediment has wide application prospects. Although other remediation technologies such as advanced oxidation processes (AOPs) can also efficiently remove atrazine from soil, some potential problems still need to be further clarified. Finally, some related challenges and prospects are proposed.

A critical review on interaction of microplastics with organic contaminants in soil and their ecological risks on soil organisms.

The pollution of microplastics (MPs) in soil has become a global environmental problem. Due to high sorption capacity and persistence in environment, the MPs exhibit combined effects with organic pollutants in soil, thereby posing a potential risk to soil ecology and human health. However, limited reviews are available on this subject. Therefore, in response to this issue, this review provides an in-depth account of interaction of MPs with organic contaminants in soil and the combined risks to soil environment. The sorption of organic contaminants onto MPs is mainly through hydrophobic and π-π interactions, hydrogen bonding, pore filling and electrostatic and van der Waals forces. The intrinsic characteristics of MPs, organic contaminants and soil are the key factors influencing the sorption of organic pollutants onto MPs. Importantly, the presence of MPs changes the sorption, degradation and transport behaviors of organic contaminants in soil, and affects the toxic effects of organic contaminants on soil organisms including animals, plants and soil microorganisms through synergistic or antagonistic effects. Source control, policy implementation and plastic removal are the main preventive and control measures to reduce soil MPs pollution. Finally, priorities for future research are proposed, such as field investigations of co-pollution, contribution of plastisphere to organic contaminant degradation, and mechanisms of MPs effects on organic contaminant toxicity.

Long-term rumen microorganism fermentation of corn stover in vitro for volatile fatty acid production.

Rumen microorganisms have the ability to efficiently hydrolyze and acidify lignocellulosic biomass. The effectiveness of long-term rumen microorganism fermentation of lignocellulose in vitro for producing volatile fatty acids (VFAs) is unclear. The feasibility of long-term rumen microorganism fermentation of lignocelluose was evaluated in this study, and a stable VFA production was successfully realized for 120 d. Results showed that VFA concentration reached to 5.32-8.48 g/L during long-term fermentation. Hydrolysis efficiency of hemicellulose and cellulose reached 36.5%-52.2% and 29.4%-38.4%, respectively. A stable bacterial community was mainly composed of Prevotella, Rikenellaceae_RC9_gut_group, Ruminococcus, and Succiniclasticum. VFA accumulation led to a pH decrease, which caused the change of bacterial community structure. Functional prediction showed that the functional genes related to hydrolysis and acidogenesis of corn stover were highly expressed during long-term fermentation. The successful long-term rumen fermentation to produce VFAs is of great significance for the practical application of rumen microorganisms.

Improvement of Direct Interspecies Electron Transfer via Adding Conductive Materials in Anaerobic Digestion: Mechanisms, Performances, and Challenges.

Anaerobic digestion is an effective and sustainable technology for resource utilization of organic wastes. Recently, adding conductive materials in anaerobic digestion to promote direct interspecies electron transfer (DIET) has become a hot topic, which enhances the syntrophic conversion of various organics to methane. This review comprehensively summarizes the recent findings of DIET mechanisms with different mediating ways. Meanwhile, the influence of DIET on anaerobic digestion performance and the underlying mechanisms of how DIET mediated by conductive materials influences the lag phase, methane production, and system stability are systematically explored. Furthermore, current challenges such as the unclear biological mechanisms, influences of non-DIET mechanisms, limitations of organic matters syntrophically oxidized by way of DIET, and problems in practical application of DIET mediated by conductive materials are discussed in detail. Finally, the future research directions for practical application of DIET are outlined.

Spent Ganoderma lucidum substrate derived biochar as a new bio-adsorbent for Pb2+/Cd2+ removal in water.

The present study firstly reports spent Ganoderma lucidum substrate derived biochars (SLBCS) for the effective removal of Pb2+/Cd2+ from water. The effects of pyrolysis temperature on the SLBCS characteristics and Pb2+/Cd2+ adsorption mechanism was studied systematically. The surface physicochemical properties of SLBCS were significantly affected by the pyrolysis temperature. The increase in pyrolysis temperature from 250 to 650 °C resulted in a drastic increase in the biochar surface area and the well development of mesoporous structure, which could provide more effective adsorption sites for Pb2+ and Cd2+ onto SLBCS. According to the Langmuir model, the obtained maximum adsorption capacity of Pb2+ onto SL650 reached 262.76 mg g-1, while that of Cd2+ reached 75.82 mg g-1. The adsorption capacities of SL650 for Pb2+ and Cd2+ were even higher than that of other modified biochars. The high adsorption capacity of SL650 for Pb2+, attributed to the precipitation supported by high temperature, benefitted the formation of carbonate minerals. Two possible mechanisms involved in Cd2+ sorption: carbonate precipitation and coordination with π electrons. Desorption of SL650 showed high efficiency for Pb2+, but slightly low efficiency for Cd2+. These results indicate that SL650 can be applied for removing heavy metals, especially Pb2+, from polluted water.