ABSTRACT
A cuticle protein gene, AyCP12, from the Japanese oak silkmoth, Antheraea yamamai, was isolated and characterized. The gene spans 1107 bp and consists of one intron and two exons coding for a 112 amino acid polypeptide with a predicted molecular mass of 12,163 Da and a pI of 4.4. The AyCP12 protein contained a type-specific consensus sequence identifiable in other insect cuticle proteins and the deduced amino acid sequence of the AyCP12 cDNA is most homologous to another silkmoth, A. pernyi, cuticle protein ApCP13 (82% protein sequence identity). Northern blot analysis revealed that AyCP12 showed the epidermis-specific expression.
Subject(s)
Bombyx/genetics , DNA, Complementary/chemistry , Insect Proteins/genetics , Amino Acid Sequence , Animals , Cloning, Molecular , Conserved Sequence , DNA, Complementary/isolation & purification , Gene Expression Profiling , Gene Expression Regulation, Developmental , Gene Library , Insect Proteins/metabolism , Larva , Molecular Sequence Data , RNA, Messenger/isolation & purification , RNA, Messenger/metabolism , Sequence Analysis, DNA , Sequence Homology, Amino AcidABSTRACT
LIM protein cDNA, from Bombyx mori that contains an open reading frame of 622 bp encoding 94 amino acids, was identified and characterized. The B. mori LIM protein homologue is classified into group 2 LIM proteins that contain glycine-rich LIM domain. B. mori LIM protein mRNA is up-regulated at late embryogenesis and detected in the mid-gut of 5th instar larvae.
