ABSTRACT
An organocuprate(III) complex, [Cu(TMSPS3)(CH2CN)]- (2), was identified along with a sequential derivative, [Cu(TMSPS3)(CN)]- (3), and an intermediate, [Cu(TMSPS3)(HNâPPh3)] (4), formed in a relative transformation. Apical ligands among these complexes all strongly associate with a robust trigonal copper(III) platform. The nature of the ligand binding was spectroscopically and computationally investigated through a series of copper(III) complexes. The bonding along the principal C3 axis is adaptable, and σ interaction dominates the axial ligand coordination, where the cyanomethyl group exhibits the strongest bonding. Complex 2 is a scarce example of a thermostable aliphatic organocuprate(III) compound, which sheds some light on the organocopper(III) chemistry widely involved in many copper-mediated catalyses.
ABSTRACT
In this study, the first nestin isoform, Nes-S, was identified in neurons of dorsal root ganglia (DRG) of adult rats. Nes-S cannot form filaments by itself in cytoplasmic intermediate filament-free SW13 cells. Instead, it co-assembles into filaments with vimentin when transfected into vimentin(+) SW13 cells, and with peripherin and neurofilament proteins when transfected into N2a cells. In primary DRG neurons, endogenous Nes-S co-assembles with peripherin and neurofilament proteins. The expression of Nes-S first appears in DRG at postnatal day 5 and persists to adulthood. Among the adult tissues we examined, the expression of Nes-S is restricted to the sensory and motor neurons. Finally, exogenous Nes-S enhances viability when transfected into N2a cells, and knockdown of endogenous Nes-S impairs the survival of DRG neurons in primary cultures. Taken together, Nes-S is a new neuronal intermediate filament protein that exerts a cytoprotective function in mature sensory and motor neurons.
