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1.
Zhongguo Ying Yong Sheng Li Xue Za Zhi ; 38(5): 555-558, 2022 Sep.
Article in Chinese | MEDLINE | ID: mdl-37088769

ABSTRACT

OBJECTIVE: To investigate the effect of hydroxysafflower yellow A (HSYA) on pulmonary fibrosis induced by bleomycin in mice and transforming growth factor ß 1(TGF-ß1) /Smad signal transduction pathway regulation. METHODS: The pulmonary fibrosis model was prepared by intranasal injection of bleomycin 50 µl (15 mg/kg). ICR mice were randomly divided into control group, model group, HSYA group(6 mg/kg) and dexamethasone (Dex) group(3 mg/kg), with 15 mice in each group. From the next day of modeling, HSYA and Dex groups were intraperitoneally injected with corresponding drugs, while the control group and model group were intraperitoneally injected with the same volume of normal saline, once a day, for 28 consecutive days. After 4 weeks, the mice were sacrificed and the lungs were collected. HE and Masson staining were used to observe the pathological damage of lung tissue; Immunohistochemistry, RT-qPCR and Western blot were used to detect the expressions of TGF-ß1/Smad signaling pathway in lung tissues. RESULTS: Compared with the control group, the model group showed severe alveolitis and pulmonary fibrosis. The mRNA and protein expressions of TGF-ß1 and Smad3 in lung tissues were increased significantly (P<0.01), while the mRNA and protein expressions of Smad7 were decreased significantly (P<0.01). Compared with the model group, the degree of alveolitis and pulmonary fibrosis in the HSYA and Dex groups was reduced significantly. The mRNA and protein expressions of TGF-ß1 and Smad3 in lung tissues of HSYA and Dex groups were decreased significantly (P<0.01), while the mRNA and protein expressions of Smad7 were increased significantly(P<0.01). CONCLUSION: HSYA can alleviate the pathogenesis of pulmonary fibrosis, and its mechanism may be related to the regulation of TGF-ß1/Smad signaling pathway.


Subject(s)
Pulmonary Fibrosis , Mice , Animals , Pulmonary Fibrosis/chemically induced , Transforming Growth Factor beta1/metabolism , Mice, Inbred ICR , Bleomycin/adverse effects , RNA, Messenger/metabolism , Fibrosis
2.
Int J Toxicol ; 38(5): 436-444, 2019.
Article in English | MEDLINE | ID: mdl-31342801

ABSTRACT

Triptolide is a major active ingredient isolated from the traditional Chinese herb Tripterygium wilfordii Hook F. However, its use in clinical practice is limited due to its severe hepatotoxicity. Autophagy, a highly conserved intracellular process, is essential for maintaining cytoplasmic homeostasis. Considering that abnormalities in autophagy are closely associated with drug-mediated hepatotoxicity, we applied human normal liver HL7702 cells to elucidate the roles of autophagy in triptolide-induced hepatotoxicity. Our study revealed that triptolide was cytotoxic to HL7702 cells. It markedly increased autophagosome formation and expression of autophagy-related proteins, namely Beclin1 and microtubule-associated protein 1 light chain 3II, and induced oxidative stress. These proautophagic effects were counteracted by pretreatment with N-acetylcysteine, a reactive oxygen species scavenger. Moreover, the pharmacological suppression of autophagy further exacerbated triptolide-elicited decrease in cell viability, increase in lactate dehydrogenase leakage, and activation of apoptosis proteases (caspase 3 and caspase 9). Our findings suggest that triptolide-induced oxidative stress consequently enhances autophagic activity, and autophagy is a cytoprotective mechanism against triptolide-induced cytotoxicity in HL7702 cells.


Subject(s)
Autophagy/drug effects , Diterpenes/toxicity , Hepatocytes/drug effects , Phenanthrenes/toxicity , Caspase 3/metabolism , Caspase 9/metabolism , Cell Line , Epoxy Compounds/toxicity , Glutathione/metabolism , Hepatocytes/metabolism , Hepatocytes/ultrastructure , Humans , Malondialdehyde/metabolism , Microscopy, Electron, Transmission , Oxidative Stress/drug effects , Reactive Oxygen Species/metabolism , Superoxide Dismutase/metabolism
3.
Spectrochim Acta A Mol Biomol Spectrosc ; 183: 131-137, 2017 Aug 05.
Article in English | MEDLINE | ID: mdl-28441540

ABSTRACT

The supramolecular interaction between cucurbit[7]uril (CB[7]) as the host and the anti-cancer drug methotrexate (MTX) as the guest was studied using fluorescence spectroscopy, UV-visible absorption spectroscopy, 1H NMR, 2D NOESY, and theoretical calculations. The experimental results confirmed the formation of 1:2 inclusion complex with CB[7] and indicated a simple and sensitive competitive method for the fluorescence detection of MTX. It was found that the fluorescence intensities of CB[7]-palmatine, CB[7]-berberine and CB[7]-coptisine were quenched linearly upon the addition of MTX. The linear ranges obtained in the detection of MTX were 0.1-15µgmL-1, 0.2-15µgmL-1, and 0.4-15µgmL-1 with detection limits of 0.03µgmL-1, 0.06µgmL-1, and 0.13µgmL-1, respectively. This method can be used for the determination of MTX in biological fluids. These results suggested that cucurbit[7]uril is a promising drug carrier for targeted MTX delivery and monitoring, with improved efficacy and reduced toxicity in normal tissues.


Subject(s)
Bridged-Ring Compounds/metabolism , Imidazoles/metabolism , Methotrexate/analysis , Methotrexate/metabolism , Bridged-Ring Compounds/chemistry , Drug Carriers/chemistry , Drug Carriers/metabolism , Imidazoles/chemistry , Limit of Detection , Linear Models , Methotrexate/chemistry , Spectrometry, Fluorescence
4.
Spectrochim Acta A Mol Biomol Spectrosc ; 162: 98-104, 2016 Jun 05.
Article in English | MEDLINE | ID: mdl-26994318

ABSTRACT

A complexation of non-fluorescent phenformin hydrochloride (PFH) with cucurbit [7]uril (CB [7]) in aqueous solution was investigated using the fluorescent probe of palmatine (PAL) coupled with CB [7]. The fluorescent probe of CB [7]-PAL exhibited strong fluorescence in aqueous solution, which was quenched gradually with the increase of PFH. This effect is observed because when PFH was added to the host-guest system of CB [7]-PAL, PFH and PAL competed to occupy the CB [7] cavity. Portions of the PAL molecule were expelled from the CB [7] cavity owing to the introduction of PFH. Based on the significant quenching of the supramolecular complex fluorescence intensity, a fluorescence method of high sensitivity and selectivity was developed to determine PFH with good precision and accuracy for the first time. The linear range of the method was 0.005-1.9 µg mL(-1) with a detection limit of 0.003 µg mL(-1). In this work, association constants (K) of PFH with CB [7] were also determined. KCB [7]-PFH=(2.52±0.05)×10(5) L mol(-1). The ability of PFH to bind with CB [7] is stronger than that of PAL. The results of a density functional theory calculation authenticated that the moiety of PFH was embedded in the hydrophobic cavity of CB [7] tightly, and the nitrogen atom is located in the vicinity of a carbonyl-laced portal in the energy-minimized structure. The molecular modelling of the interaction between PFH and CB [7] was also confirmed by (1)H NMR spectra (Bruker 600 MHz).


Subject(s)
Bridged-Ring Compounds/chemistry , Fluorescent Dyes/chemistry , Imidazoles/chemistry , Models, Chemical , Phenformin/analysis , Phenformin/chemistry , Magnetic Resonance Spectroscopy
5.
J Fluoresc ; 24(1): 143-52, 2014 Jan.
Article in English | MEDLINE | ID: mdl-23900848

ABSTRACT

The complex characteristics of p-sulfonated calix[n]arene (SCnA) and two tryptophans N-[(tert-butoxy) carbonyl]-tryptophan (trp-A) and N-carbobenzoxy-tryptophane (trp-B) were examined through various techniques. Spectrofluorimetry was performed at different temperatures to determine the stability constants and evaluate the thermodynamic parameters of the two complexes. The effect of pH on complex formation was estimated. According to the fluorescence data, the assumption about the steric hindrance of the tert-butyl group of trp-A and the phenyl group of trp-B was put forward. (1)H NMR was also performed to determine the binding interaction mechanism. Results showed that the indole benzene rings of the two tryptophans partly penetrated into the cavity of p-sulfonated calix[n]arene. The shift in Ha, Hb and Hc, Hd positions became more significant as the number of phenolic units of the calixarene ring increased. Molecular modeling of the complexes elucidated the assumption about the steric hindrance of the tert-butyl group of trp-A and the phenyl group of trp-B. These observations of molecular modeling computation are consistent with previous fluorescence data and (1)H NMR results.


Subject(s)
Calixarenes/chemistry , Sulfonic Acids/chemistry , Tryptophan/chemistry , Macromolecular Substances/chemistry , Models, Molecular , Molecular Structure , Spectrometry, Fluorescence
6.
Article in English | MEDLINE | ID: mdl-22959366

ABSTRACT

Amantadine hydrochloride (AMA) and rimantadine hydrochloride (RIM) are non-fluorescent in aqueous solutions. This property makes their determination through direct fluorescent method difficult. The competing reactions and the supramolecular interaction mechanisms between the two drugs and coptisine (COP) as they fight for occupancy of the cucurbit[7]uril (CB[7]) cavity, were studied using spectrofluorimetry, (1)H NMR, and molecular modeling calculations. Based on the significant quenching of the supramolecular complex fluorescence intensity, a fluorescent probe method of high sensitivity and selectivity was developed to determine AMA or RIM in their pharmaceutical dosage forms and in urine samples with good precision and accuracy. The linear range of the method was from 0.0040 to 1.0 µg mL(-1) with a detection limit ranging from 0.0012 to 0.0013 µg mL(-1). This shows that the proposed method has promising potential for therapeutic monitoring and pharmacokinetics and for clinical application.


Subject(s)
Amantadine/analysis , Analgesics, Non-Narcotic/analysis , Antiviral Agents/analysis , Fluorescent Dyes/chemistry , Rimantadine/analysis , Limit of Detection , Spectrometry, Fluorescence/methods
7.
Analyst ; 136(20): 4168-73, 2011 Oct 21.
Article in English | MEDLINE | ID: mdl-21858296

ABSTRACT

A validated, simple, and sensitive fluorescence quenching method for the determination of ranitidine, nizatidine, and cimetidine in tablets and biological fluids is presented. This is the first single fluorescence method reported for the analysis of all three H(2) antagonists. The competitive reaction between the investigated drug and the palmatine probe for the occupancy of the cucurbit[7]uril (CB[7]) cavity was studied using spectrofluorometry. CB[7] was found to react with the probe to form a stable complex. The fluorescence intensity of the complex was also enhanced greatly. However, the addition of the drug dramatically quenched the fluorescence intensity of the complex. Accordingly, a new fluorescence quenching method for the determination of the studied drugs was established. The different experimental parameters affecting the fluorescence quenching intensity were studied carefully. At optimum reaction conditions, the rectilinear calibration graphs between the fluorescence quenching values (ΔF) and the medicament concentration were obtained in the concentration range of 0.04-1.9 µg mL(-1) for the investigated drugs. The limits of detection ranged from 0.013 to 0.030 µg mL(-1) at 495 nm using an excitation wavelength of 343 nm. The proposed method can be used for the determination of the three H(2) antagonists in raw materials, dosage forms and biological fluids.


Subject(s)
Fluorescent Dyes/chemistry , Histamine H2 Antagonists/analysis , Spectrometry, Fluorescence , Bridged-Ring Compounds/chemistry , Cimetidine/analysis , Cimetidine/urine , Histamine H2 Antagonists/urine , Humans , Hydrogen-Ion Concentration , Imidazoles/chemistry , Nizatidine/analysis , Nizatidine/urine , Ranitidine/analysis , Ranitidine/urine , Temperature
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