ABSTRACT
No disponible
Subject(s)
Humans , Male , Middle Aged , Agrobacterium tumefaciens , Agrobacterium tumefaciens/isolation & purification , Agrobacterium tumefaciens/radiation effects , Lung Abscess/diagnosis , Lung Abscess/microbiology , Lung Abscess , Pneumonia/complications , Pneumonia/drug therapy , Anti-Bacterial Agents/analysis , Anti-Bacterial Agents/biosynthesis , Tomography, Emission-Computed/methods , Microbial Sensitivity Tests/methodsABSTRACT
Introducción. Comparar las pruebas de antígeno galactomanano (AG) y moleculares (PCRrt) con el cultivo para el diagnóstico de aspergilosis invasiva (AI). Material y métodos. Se analizaron 472 muestras: 388 respiratorias, 84 sueros, de 271 pacientes. En las respiratorias se realizó cultivo y AG y en los sueros AG. En caso de discordancia entre ellas se realizó PCR. Resultados. El AG resultó positivo en 22 sueros de 84, 21 tenían esta prueba positiva en muestra respiratoria. De 62 sueros con AG negativo, 45 fueron también negativas en muestras respiratorias. El cultivo fue positivo en 37, coincidiendo todas con AG positivo. Comparando cultivo con AG, éste mostró un VPP= 23%, VPN=100%, S= 100% y E=52%. La PCRrt respecto al cultivo mostró un VPP= 69%, VPN= 89%, S= 64% y E= 82%. En los sueros entre AG y PCR encontramos 60% de discrepancias. Conclusiones. Consideramos muy útil la detección de AG en suero combinada con cultivo y AG en muestras respiratorias para el diagnóstico de AI, precisando PCRrt más estudios para su estandarización y establecer puntos de corte (AU)
Introduction. The aim of the study was to compare the galactomannan antigen (GA) and molecular biology (PCRrt) tests with the culture in the diagnosis of invasive aspergillosis (IA). Material and methods. Four hundred and seventy two samples were analyzed: 388 respiratory and 84 serum samples from 271 patients. Culture and GA were evaluated in the respiratory samples and GA in the serum samples. PCR was used when discrepancies were observed among culture and GA tests. Results. The detection of GA in serum was positive in 22 (of 84), 21 had the test positive respiratory sample. Of the 62 sera with negative GA, 45 were also negative respiratory specimens. The culture was positive in 37 of which were positive for GA. Comparing culture with AG, it showed PPV=23%, NPV=100%, S=100% and E=52%. The PCR showed respect to culture: PPV=69%, NPV=89%, S=64% and E=82%. In sera were found in 60% discrepancies between PCRrt and GA. Conclusions. We consider useful the GA detection in serum combined with culture and GA in respiratory samples, for diagnosis of AI. PCR requires further studies for standardization and set breakpoints (AU)
