ABSTRACT
A new macrocyclic diterpenoid, 4ß,5ß-dihydroxyovatodiolide (1), together with twenty-two known compounds (2-23) were isolated from the MeOH extract of the dried aerial parts of Anisomeles indica (L.) O. Kuntze (Labiatae). The structure of 1 was established on the basis of spectral evidence. Phenylethanoids, acteoside (5) and isoacteoside (6) showed significant inhibitory to IL-2 secretion of with respect to phorbol myristate acetate and anti-CD28 monoclonal antibody co-stimulated activation of human peripheral blood T cells.
Subject(s)
Diterpenes/chemistry , Diterpenes/pharmacology , Lamiaceae/chemistry , Antibodies, Monoclonal/pharmacology , CD28 Antigens/immunology , CD28 Antigens/metabolism , Drug Evaluation, Preclinical/methods , Drugs, Chinese Herbal/chemistry , Humans , Interleukin-2/metabolism , Macrocyclic Compounds/chemistry , Magnetic Resonance Spectroscopy , Molecular Structure , Plant Components, Aerial/chemistry , Plant Extracts/chemistry , T-Lymphocytes/drug effects , T-Lymphocytes/metabolism , Tetradecanoylphorbol Acetate/pharmacologyABSTRACT
Three new ubiquinone derivatives, antrocamol LT1, antrocamol LT2, and antrocamol LT3, along with two known compounds, were isolated from Antrodia camphorata (Polyporaceae) mycelium. The structures of these compounds were established on the basis of extensive 1D and 2D NMR spectroscopic analyses. These ubiquinones exhibited selective cytotoxicities against five human cancer cell lines (CT26, A549, HepG2, PC3 and DU-145) with IC50 values ranging from 0.01 to 1.79µΜ.
