Subject(s)
HIV-1/drug effects , Polychlorinated Dibenzodioxins/pharmacology , Virus Replication/drug effects , Cell Line , Dose-Response Relationship, Drug , Enzyme Induction , HIV-1/enzymology , HIV-1/physiology , Humans , Lymphocytes/microbiology , RNA-Directed DNA Polymerase/biosynthesis , Stimulation, ChemicalSubject(s)
HIV-1/drug effects , HLA Antigens/chemistry , Peptides/pharmacology , Receptors, HIV/chemistry , Virus Replication/drug effects , Amino Acid Sequence , Cell Line , HIV-1/physiology , Humans , Molecular Sequence Data , Peptide T/pharmacology , Peptides/chemical synthesis , Sequence Homology, Nucleic Acid , Viral Envelope Proteins/chemistryABSTRACT
Interferon-inducing and antiviral effects of natural dsRNA preparations of phage phi 6 and yeast cells were studied in the culture of murine cells L-929 and on random bred albino mice. Both the preparations showed interferon inducing activity in the cell culture. However, for realization of their effect modification of the surface cell membrane by polycation exchange resin (DEAE-dextran) was required. The interferon-inducing activity of both of the natural dsRNA in the mice was high. The maximum interferon titers (1280-5120 units/ml) in blood serum were observed 4-6 hours after the inductor intraperitoneal administration. The interferon-inducing activity of the phage dsRNA was high in the cell culture and yeast dsRNA--in mice, respectively. Both the inductors had antiviral activity and protected 15 to 38.9 per cent of the experimental animals from the effect of 100 LD50 of the murine encephalomyocarditis virus and 10 LD50 of the influenza virus A/Aichi 2/68 (H3N2).
Subject(s)
Interferon Inducers/pharmacology , Animals , Cytopathogenic Effect, Viral/drug effects , DEAE-Dextran/pharmacology , DNA/isolation & purification , DNA/pharmacology , DNA/toxicity , Encephalomyocarditis virus/drug effects , Influenza A virus/drug effects , Interferon Inducers/isolation & purification , Interferon Inducers/toxicity , Interferons/blood , L Cells/drug effects , Lethal Dose 50 , Mice , RNA, Fungal/isolation & purification , RNA, Fungal/pharmacology , RNA, Fungal/toxicity , RNA, Viral/isolation & purification , RNA, Viral/pharmacology , RNA, Viral/toxicity , Time FactorsABSTRACT
Effect of bilateral lesions of suprachiasmatic nuclei (SCN) of anterior hypothalamus on circadian rhythms of locomotor activity in a treadmill was studied in blinded rats. Immediately after the SCN lesion the free-run circadian rhythm of locomotor activity disappeared: the animals became arrhythmic. Partial lesion of SCN weakened the circadian component of rhythm and led to irregular oscillations within 0.5--3.5 hrs range which put on circadian periodicity. Significance of the anterior hypothalamus SCN in organization of circadian rhythms in rats was discussed.
Subject(s)
Circadian Rhythm , Motor Activity/physiology , Suprachiasmatic Nucleus/physiology , Animals , Male , Rats , Vision, OcularSubject(s)
Conditioning, Classical , Hibernation , Seasons , Animals , Avoidance Learning , Reaction Time , SciuridaeSubject(s)
Amnesia/drug therapy , Galantamine/therapeutic use , Amnesia/chemically induced , Animals , Avoidance Learning , Humans , Male , Mice , Mice, Inbred BALB C , ScopolamineABSTRACT
In experiments on mice of the BALB/c strain a study was made of the influence of lysergic acid diethylamide (LSD) on the retention and reproduction of a conditioned passive avoidance reaction (CPAR). A 0.5-3.0 mg/kg of LSD Injected intraperitoneally 10 min. before the learning procedure, worsened the CPAR retention, while a 0.2 mg/kg of LSD Improved it. The drug (0.2 mg/kg) facilitated the retrieval of the reaction, which did not manifest itself in 24 and 48 hours after learning. This effect depended on the strength of the unconditioned stimulus presented during learning as well as on the time intervals between the moments of learning and testing. Facilitated CPAR retrieval was observed only during the action of the drug and disappeared in 24 hours after its administration. The possible physiological mechanisms of LSD influence on memory processes are discussed.
