ABSTRACT
We report the identification of a novel major histocompatibility complex class I-related chain (MICB) allele, provisionally designated as MICB-0114 pending the WHO Nomenclature Classification for the MICB locus. This new allele is identical to MICB-0103101v except for a single mutation of G to A in exon 4 that translates into an amino acid substitution from glutamic acid to lysine.
Subject(s)
Genes, MHC Class I , Proteins/genetics , Alleles , Amino Acid Substitution , Base Sequence , DNA/genetics , DNA Primers/genetics , Exons , Histocompatibility Antigens Class I , Humans , Molecular Sequence Data , Point Mutation , Polymerase Chain Reaction , Sequence Homology, Nucleic AcidABSTRACT
We have identified a novel MICA allele, MICA*051, detected by the polymerase chain reaction using sequence-specific primers and characterized by sequence-based typing. MICA*051 appears to be the result of a recombination between MICA*00801 and MICA*00701 at intron 2.
Subject(s)
Histocompatibility Antigens Class I/genetics , Alleles , Base Sequence , DNA Primers , Exons , Humans , Introns , Molecular Sequence Data , Polymerase Chain Reaction , Polymorphism, GeneticABSTRACT
The presence of an unusual HLA class I reactivity pattern was detected in a Caucasoid-Asian individual by PCR-sequence specific primer (PCR-SSP) typing. Exons 2 and 3 were characterized using PCR-sequence-based typing (PCR-SBT) and were found to contain a novel Cw*03 sequence, Cw*0315. In the region studied, Cw*0315 was comprised mainly of the Cw*0302 sequence, but at four positions it contained nucleotides normally only found in other HLA Cw locus alleles. These positions each resulted in an amino acid substitution.
