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1.
Rev Neurol (Paris) ; 179(5): 378-393, 2023 Jun.
Article in English | MEDLINE | ID: mdl-37030987

ABSTRACT

The majority of intracranial expansive lesions are tumors. However, a wide range of lesions can mimic neoplastic pathology. Differentiating pseudotumoral lesions from brain tumors is crucial to patient management. This article describes the most common intracranial pseudotumors, with a focus on the imaging features that serve as clues to detect pseudotumors.


Subject(s)
Brain Neoplasms , Humans , Diagnosis, Differential , Brain Neoplasms/diagnostic imaging
3.
Vet Microbiol ; 42(4): 349-59, 1994 Dec.
Article in English | MEDLINE | ID: mdl-9133060

ABSTRACT

An enzyme-linked immunosorbent assay (ELISA) for the detection of transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV) was developed. A bovine TGEV-specific polyclonal antibody was purified by affinity chromatography with the TRIO Bioprocessing System and was used as the capture antibody, at a concentration of 1.5 micrograms/well. The F5.39 monoclonal antibody was obtained by the fusion of spleen lymphocytes from TGEV immunized mice with NS-1 myeloma cells. This mAb was used as a second antibody for the ELISA. The ELISA detected 40 ng of TGEV and 407 ng of PRCV. To study the ability of ELISA to detect TGEV in field cases, 53 intestinal samples were taken from pigs exhibiting clinical signs of transmissible gastroenteritis. All the positive samples detected by the ELISA were confirmed as positive by immunofluorescence or cell culture immunofluorescence. To study the ability of this ELISA to detect PRCV in nasal swabs and lung samples, 20 seven-day-old piglets were inoculated with a Quebec strain of PRCV. The ELISA was able to detect PRCV in both kinds of samples.


Subject(s)
Coronavirus Infections/veterinary , Coronavirus/isolation & purification , Enzyme-Linked Immunosorbent Assay/veterinary , Gastroenteritis, Transmissible, of Swine/virology , Lung Diseases/veterinary , Swine Diseases/virology , Transmissible gastroenteritis virus/isolation & purification , Animals , Coronavirus Infections/virology , Enzyme-Linked Immunosorbent Assay/methods , Feces/microbiology , Lung/virology , Lung Diseases/virology , Mice , Mice, Inbred BALB C , Nasal Lavage Fluid/microbiology , Sensitivity and Specificity , Swine
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