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Real-time PCR detection of gyrA and parC mutations in Streptococcus pneumoniae.
Page, S; Vernel-Pauillac, F; O'Connor, O; Bremont, S; Charavay, F; Courvalin, P; Goarant, C; Le Hello, S.
Antimicrob Agents Chemother ; 52(11): 4155-8, 2008 Nov.
Article in English | MEDLINE | ID: mdl-18725440

ABSTRACT

Fluoroquinolone resistance in Streptococcus pneumoniae mainly involves stepwise mutations predominantly in the parC and gyrA genes. We have developed a single-run real-time PCR assay for detection of the four most common mutations in the quinolone resistance-determining regions of these genes. This assay provides a useful tool for both clinical and epidemiological use.


Subject(s)
DNA Gyrase/genetics , DNA Topoisomerase IV/genetics , Polymerase Chain Reaction/methods , Streptococcus pneumoniae/enzymology , Streptococcus pneumoniae/genetics , Amino Acid Substitution , Base Sequence , DNA Gyrase/chemistry , DNA Mutational Analysis/methods , DNA Primers/genetics , DNA Probes/chemistry , DNA Probes/genetics , DNA Topoisomerase IV/chemistry , DNA, Bacterial/genetics , Drug Resistance, Bacterial/genetics , Fluoroquinolones/pharmacology , Genes, Bacterial , Humans , Nucleic Acid Denaturation , Point Mutation , Streptococcus pneumoniae/drug effects , Streptococcus pneumoniae/isolation & purification , Thermodynamics
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