ABSTRACT
AIM: To determine the effect of the surface roughness of denture acrylic on the attachment of Streptococcus oralis. METHODS AND RESULTS: Roughened denture acrylic samples were assessed for bacterial attachment, over time, using microscopy. The area of the image covered by bacteria was calculated and converted into a percentage of the total area sampled. The results showed an increasing bacterial coverage with time of incubation and increasing roughness. Differences were seen between heat cured acrylic and cold cured acrylic. CONCLUSION: This study successfully demonstrated a system for the assessment of the amount of attached bacteria on denture acrylic varying roughness. The system was able to discern the difference in surface area coverage by attached bacteria over a roughness range relevant to brushing dentures with dentifrices. SIGNIFICANCE AND IMPACT OF STUDY: This study provides strong support for the scratches caused by brushing dentures with dentifrice encouraging bacterial attachment. This is likely to have a significant effect on efficacy of denture cleaning, general hygiene and biofilm re-formation between cleaning regimens and may indicate that alternative low abrasive cleaners, such as antimicrobial denture-cleaning tablets, offer a more appropriate regimen.
Subject(s)
Acrylic Resins/chemistry , Bacterial Adhesion , Dentures , Streptococcus oralis/physiology , Cold Temperature , Colony Count, Microbial , Dentifrices , Hot Temperature , Humans , Surface Properties , Toothbrushing , ToothpastesABSTRACT
The choice of small-scale fermentation systems contributes significantly to a successful scale-up. Creasing of flasks and the chosen shaker parameters influence the production of secondary metabolites in a strain- and even compound-specific manner. Using actinomycetes and fungi as model organisms the influence of the small-scale fermentation system on the production of various secondary metabolites is described and the effects on screening success and scale-up are considered.
