Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Arch Dermatol ; 147(3): 286-91, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21422334

ABSTRACT

OBJECTIVE: To investigate the diagnostic value of commercially available BP230 and BP180-NC16a enzyme-linked immunosorbent assays (ELISAs) in routine practice in patients with bullous pemphigoid (BP). DESIGN: Single-center retrospective study. SETTING: French academic dermatology department. PATIENTS: The study population comprised 138 patients, who were admitted from January 1998 through December 2008. INTERVENTIONS: Sera samples were analyzed by ELISA; clinical and immunopathological data were recorded from the patients' medical charts. MAIN OUTCOME MEASURES: BP230 and BP180-NC16a ELISA scores were evaluated with respect to clinical characteristics (number of blisters, mucosal involvement, localized or generalized disease, and outcome) and routine indirect immunofluorescence (IF). RESULTS: Of the 138 study patients, 81 (59%) had a positive BP230 ELISA result and 119 (86%) had a positive BP180 ELISA result. There was no relationship between a positive ELISA BP230 result and the disease extent at diagnosis or the presence of mucosal involvement. Serum anti-basement membrane zone autoantibodies (indirect IF) were more frequently detected when the BP230 ELISA result was positive (P < .001). The median anti-basement membrane autoantibody titer as detected by indirect IF was higher in patients with a positive BP230 result (P < .001). The BP180 ELISA result was associated with disease extent at diagnosis as estimated by both the percentage of patients with extensive BP (P = .01) and the mean number of blisters (P = .03) but was not associated with mucosal involvement. CONCLUSIONS: The currently available BP230 ELISA is a reliable although less-sensitive test than BP180 ELISA in BP, and its diagnostic added value compared with BP180 ELISA alone is approximately 5%. Our results support the predominant contribution of the BP230-specific autoantibodies to anti-basement membrane zone antibody titer as detected by indirect IF.


Subject(s)
Autoantigens/immunology , Carrier Proteins/immunology , Cytoskeletal Proteins/immunology , Enzyme-Linked Immunosorbent Assay/methods , Nerve Tissue Proteins/immunology , Non-Fibrillar Collagens/immunology , Pemphigoid, Bullous/diagnosis , Aged , Autoantibodies/immunology , Dystonin , Female , Follow-Up Studies , France , Humans , Male , Pemphigoid, Bullous/immunology , Pemphigoid, Bullous/physiopathology , Retrospective Studies , Sensitivity and Specificity , Collagen Type XVII
SELECTION OF CITATIONS
SEARCH DETAIL
...