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1.
Cell Death Differ ; 17(10): 1577-87, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20339376

ABSTRACT

Polyglutamine pathologies are neurodegenerative diseases that manifest both general polyglutamine toxicity and mutant protein-specific effects. Dentatorubral-pallidoluysian Atrophy (DRPLA) is one of these disorders caused by mutations in the Atrophin-1 protein. We have generated several models for DRPLA in Drosophila and analysed the mechanisms of cellular and organism toxicity. Our genetic and ultrastructural analysis of neurodegeneration suggests that autophagy may have a role in cellular degeneration when polyglutamine proteins are overexpressed in neuronal and glial cells. Clearance of autophagic organelles is blocked at the lysosomal level after correct fusion between autophagosomes and lysosomes. This leads to accumulation of autofluorescent pigments and proteinaceous residues usually degraded by the autophagy-lysosome system. Under these circumstances, further pharmacological and genetic induction of autophagy does not rescue neurodegeneration by polyglutamine Atrophins, in contrast to many other neurodegenerative conditions. Our data thus provide a crucial insight into the specific mechanism of a polyglutamine disease and reveal important differences in the role of autophagy with respect to other diseases of the same family.


Subject(s)
Autophagy , Drosophila Proteins/metabolism , Myoclonic Epilepsies, Progressive/pathology , Nerve Tissue Proteins/metabolism , Transcription Factors/metabolism , Animals , Disease Models, Animal , Drosophila , Drosophila Proteins/genetics , Humans , Mutation , Myoclonic Epilepsies, Progressive/genetics , Nerve Tissue Proteins/genetics , Nerve Tissue Proteins/toxicity , Neurodegenerative Diseases/pathology , Neuroglia/cytology , Neuroglia/drug effects , Neurons/cytology , Neurons/drug effects , Peptides/toxicity , Transcription Factors/genetics
2.
Curr Biol ; 11(14): 1079-88, 2001 Jul 24.
Article in English | MEDLINE | ID: mdl-11509230

ABSTRACT

BACKGROUND: The survival of motor neurons (SMN) protein is the protein product of the spinal muscular atrophy (SMA) disease gene. SMN and its associated proteins Gemin2, Gemin3, and Gemin4 form a large complex that plays a role in snRNP assembly, pre-mRNA splicing, and transcription. The functions of SMN in these processes are mediated by a direct interaction of SMN with components of these machineries, such as Sm proteins and RNA helicase A. RESULTS: We show that SMN binds directly to fibrillarin and GAR1. Fibrillarin and GAR1 are specific markers of the two classes of small nucleolar ribonucleoprotein particles (snoRNPs) that are involved in posttranscriptional processing and modification of ribosomal RNA. SMN interaction requires the arginine- and glycine-rich domains of both fibrillarin and GAR1 and is defective in SMN mutants found in some SMA patients. Coimmunoprecipitations demonstrate that the SMN complex associates with fibrillarin and with GAR1 in vivo. The inhibition of RNA polymerase I transcription causes a transient redistribution of SMN to the nucleolar periphery and loss of fibrillarin and GAR1 colocalization with SMN in gems. Furthermore, the expression of a dominant-negative mutant of SMN (SMNDeltaN27) causes snoRNPs to accumulate outside of the nucleolus in structures that also contain components of gems and coiled (Cajal) bodies. CONCLUSIONS: These findings identify fibrillarin and GAR1 as novel interactors of SMN and suggest a function for the SMN complex in the assembly and metabolism of snoRNPs. We propose that the SMN complex performs functions necessary for the biogenesis and function of diverse ribonucleoprotein complexes.


Subject(s)
Chromosomal Proteins, Non-Histone/metabolism , Nerve Tissue Proteins/metabolism , Ribonucleoproteins, Small Nucleolar/metabolism , Arginine/chemistry , Binding Sites , Cell Nucleolus/metabolism , Conserved Sequence , Cyclic AMP Response Element-Binding Protein , Glycine/chemistry , HeLa Cells , Humans , Mutation , Nerve Tissue Proteins/chemistry , Nerve Tissue Proteins/genetics , Protein Binding , Protein Structure, Tertiary , RNA-Binding Proteins , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , SMN Complex Proteins , Transcription, Genetic
3.
J Cell Biol ; 152(1): 75-85, 2001 Jan 08.
Article in English | MEDLINE | ID: mdl-11149922

ABSTRACT

The survival motor neuron (SMN) protein, the protein product of the spinal muscular atrophy (SMA) disease gene, plays a role in the assembly and regeneration of small nuclear ribonucleoproteins (snRNPs) and spliceosomes. By nanoelectrospray mass spectrometry, we identified RNA helicase A (RHA) as an SMN complex-associated protein. RHA is a DEAH box RNA helicase which binds RNA polymerase II (pol II) and reportedly functions in transcription. SMN interacts with RHA in vitro, and this interaction is impaired in mutant SMNs found in SMA patients. Coimmunoprecipitation demonstrated that the SMN complex is associated with pol II, snRNPs, and RHA in vivo. In vitro experiments suggest that RHA mediates the association of SMN with the COOH-terminal domain of pol II. Moreover, transfection of cells with a dominant negative mutant of SMN, SMNDeltaN27, causes accumulation of pol II, snRNPs, and RHA in nuclear structures that contain the known markers of gems and coiled bodies, and inhibits RNA pol I and pol II transcription in vivo. These findings indicate a functional as well as physical association of the SMN complex with pol II and suggest a role for the SMN complex in the assembly of the pol II transcription/processing machinery.


Subject(s)
Nerve Tissue Proteins/metabolism , RNA Polymerase II/metabolism , Animals , Cyclic AMP Response Element-Binding Protein , DNA-Binding Proteins/metabolism , HeLa Cells , Humans , Mice , Nerve Tissue Proteins/genetics , RNA Helicases/metabolism , RNA Precursors/metabolism , RNA Processing, Post-Transcriptional , RNA-Binding Proteins , Ribonucleoproteins, Small Nuclear/metabolism , SMN Complex Proteins , TATA-Box Binding Protein , Transcription Factors/metabolism , Transcription, Genetic
4.
J Biol Chem ; 275(31): 23841-6, 2000 Aug 04.
Article in English | MEDLINE | ID: mdl-10816558

ABSTRACT

Spinal muscular atrophy is a common often lethal neurodegenerative disease resulting from deletions or mutations in the survival motor neuron gene (SMN). SMN is ubiquitously expressed in metazoan cells and plays a role in small nuclear ribonucleoprotein assembly and pre-mRNA splicing. Here we characterize the Schizosacharomyces pombe orthologue of SMN (yeast SMN (ySMN)). We report that the ySMN protein is essential for viability and localizes in both the cytoplasm and the nucleus. Like human SMN, we show that ySMN can oligomerize. Remarkably, ySMN interacts directly with human SMN and Sm proteins. The highly conserved carboxyl-terminal domain of ySMN is necessary for the evolutionarily conserved interactions of SMN and required for cell viability. We also demonstrate that the conserved amino-terminal region of ySMN is not required for SMN and Sm binding but is critical for the housekeeping function of SMN.


Subject(s)
Conserved Sequence , Fungal Proteins/genetics , Nerve Tissue Proteins/genetics , Schizosaccharomyces/genetics , Amino Acid Sequence , Cell Compartmentation , Cell Nucleus/chemistry , Cyclic AMP Response Element-Binding Protein , Cytoplasm/chemistry , Evolution, Molecular , Fungal Proteins/metabolism , Genes, Essential , Humans , Molecular Sequence Data , Nerve Tissue Proteins/metabolism , Protein Binding , Protein Conformation , Proto-Oncogene Proteins c-myc/metabolism , RNA-Binding Proteins , SMN Complex Proteins , Species Specificity
5.
J Cell Biol ; 148(6): 1177-86, 2000 Mar 20.
Article in English | MEDLINE | ID: mdl-10725331

ABSTRACT

The survival of motor neurons (SMN) protein, the product of the neurodegenerative disease spinal muscular atrophy (SMA) gene, is localized both in the cytoplasm and in discrete nuclear bodies called gems. In both compartments SMN is part of a large complex that contains several proteins including Gemin2 (formerly SIP1) and the DEAD box protein Gemin3. In the cytoplasm, the SMN complex is associated with snRNP Sm core proteins and plays a critical role in spliceosomal snRNP assembly. In the nucleus, SMN is required for pre-mRNA splicing by serving in the regeneration of spliceosomes. These functions are likely impaired in cells of SMA patients because they have reduced levels of functional SMN. Here, we report the identification by nanoelectrospray mass spectrometry of a novel component of the SMN complex that we name Gemin4. Gemin4 is associated in vivo with the SMN complex through a direct interaction with Gemin3. The tight interaction of Gemin4 with Gemin3 suggests that it could serve as a cofactor of this DEAD box protein. Gemin4 also interacts directly with several of the Sm core proteins. Monoclonal antibodies against Gemin4 efficiently immunoprecipitate the spliceosomal U snRNAs U1 and U5 from Xenopus oocytes cytoplasm. Immunolocalization experiments show that Gemin4 is colocalized with SMN in the cytoplasm and in gems. Interestingly, Gemin4 is also detected in the nucleoli, suggesting that the SMN complex may also function in preribosomal RNA processing or ribosome assembly.


Subject(s)
Cell Nucleolus/physiology , Cell Nucleus/physiology , Nerve Tissue Proteins/genetics , Nerve Tissue Proteins/metabolism , Nuclear Proteins/genetics , Nuclear Proteins/metabolism , Animals , Antibodies, Monoclonal , Cell Nucleolus/ultrastructure , Cell Nucleus/ultrastructure , Cyclic AMP Response Element-Binding Protein , Cytoplasm/physiology , Cytoplasm/ultrastructure , DEAD Box Protein 20 , DEAD-box RNA Helicases , Female , HeLa Cells , Humans , Minor Histocompatibility Antigens , Models, Molecular , Muscular Atrophy, Spinal/genetics , Nuclear Proteins/analysis , Oocytes/physiology , Oocytes/ultrastructure , RNA Helicases/analysis , RNA Helicases/metabolism , RNA-Binding Proteins , Ribonucleoproteins, Small Nuclear/metabolism , SMN Complex Proteins , Xenopus laevis
6.
J Cell Biol ; 147(6): 1181-94, 1999 Dec 13.
Article in English | MEDLINE | ID: mdl-10601333

ABSTRACT

The survival of motor neurons (SMN) gene is the disease gene of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The SMN protein is part of a complex containing several proteins, of which one, SIP1 (SMN interacting protein 1), has been characterized so far. The SMN complex is found in both the cytoplasm and in the nucleus, where it is concentrated in bodies called gems. In the cytoplasm, SMN and SIP1 interact with the Sm core proteins of spliceosomal small nuclear ribonucleoproteins (snRNPs), and they play a critical role in snRNP assembly. In the nucleus, SMN is required for pre-mRNA splicing, likely by serving in the regeneration of snRNPs. Here, we report the identification of another component of the SMN complex, a novel DEAD box putative RNA helicase, named Gemin3. Gemin3 interacts directly with SMN, as well as with SmB, SmD2, and SmD3. Immunolocalization studies using mAbs to Gemin3 show that it colocalizes with SMN in gems. Gemin3 binds SMN via its unique COOH-terminal domain, and SMN mutations found in some SMA patients strongly reduce this interaction. The presence of a DEAD box motif in Gemin3 suggests that it may provide the catalytic activity that plays a critical role in the function of the SMN complex on RNPs.


Subject(s)
Muscular Atrophy, Spinal/genetics , Nerve Tissue Proteins/metabolism , Organelles/chemistry , RNA Helicases/chemistry , RNA Helicases/metabolism , Amino Acid Motifs , Amino Acid Sequence , Antibodies, Monoclonal/immunology , Blotting, Western , Cloning, Molecular , Cyclic AMP Response Element-Binding Protein , Cytoplasm/chemistry , Cytoplasm/enzymology , DEAD Box Protein 20 , DEAD-box RNA Helicases , HeLa Cells , Humans , Molecular Sequence Data , Molecular Weight , Nerve Tissue Proteins/analysis , Nerve Tissue Proteins/genetics , Organelles/enzymology , Precipitin Tests , Protein Binding , RNA Helicases/genetics , RNA Helicases/immunology , RNA-Binding Proteins , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/metabolism , Ribonucleoproteins, Small Nuclear/metabolism , SMN Complex Proteins , Sequence Alignment , Sequence Deletion/genetics , Spliceosomes/chemistry , Spliceosomes/metabolism
7.
Mol Cell Biol ; 19(11): 7846-56, 1999 Nov.
Article in English | MEDLINE | ID: mdl-10523673

ABSTRACT

We have characterized the Drosophila bancal gene, which encodes a Drosophila homologue of the vertebrate hnRNP K protein. The bancal gene is essential for the correct size of adult appendages. Reduction of appendage size in bancal mutant flies appears to be due mainly to a reduction in the number of cell divisions in the imaginal discs. Transgenes expressing Drosophila or human hnRNP K are able to rescue weak bancal phenotype, showing the functional similarity of these proteins in vivo. High levels of either human or Drosophila hnRNP K protein in imaginal discs induces programmed cell death. Expression of the antiapoptotic P35 protein suppresses this phenotype in the eye, suggesting that apoptosis is the major cellular defect caused by overexpression of K protein. Finally, the human K protein acts as a negative regulator of bancal gene expression. We propose that negative autoregulation limits the level of Bancal protein produced in vivo.


Subject(s)
Apoptosis/genetics , Cell Division/genetics , Drosophila Proteins , Drosophila melanogaster/genetics , Genes, Insect , Ribonucleoproteins/genetics , Amino Acid Sequence , Animals , Animals, Genetically Modified , Drosophila melanogaster/embryology , Drosophila melanogaster/growth & development , Embryonic Development , Eye/growth & development , Gene Expression , Gene Expression Regulation , Genetic Complementation Test , Heterogeneous-Nuclear Ribonucleoprotein K , Heterogeneous-Nuclear Ribonucleoproteins , Humans , Inhibitor of Apoptosis Proteins , Molecular Sequence Data , Mutation , Sequence Homology, Amino Acid , Tissue Distribution , Viral Proteins/biosynthesis , Wings, Animal/growth & development
8.
Proc Natl Acad Sci U S A ; 96(20): 11167-72, 1999 Sep 28.
Article in English | MEDLINE | ID: mdl-10500148

ABSTRACT

Spinal muscular atrophy (SMA) is a common motor neuron degenerative disease and the leading genetic cause of death of young children. The survival of motor neurons (SMN) gene, the SMA disease gene, is homozygously deleted or mutated in more than 98% of SMA patients. The SMN protein interacts with itself, with SMN-interacting protein 1, and with several spliceosomal small nuclear ribonucleoprotein (snRNP) Sm proteins. A complex containing SMN plays a critical role in spliceosomal snRNP assembly and in pre-mRNA splicing. SMN mutants found in SMA patients show reduced self-association and lack the capacity to regenerate the splicing machinery. Here we demonstrate that SMN mutants found in SMA patients are defective in binding to Sm proteins. Moreover, we show that SMN, but not mutants found in SMA patients, can form large oligomers and that SMN oligomerization is required for high-affinity binding to spliceosomal snRNP Sm proteins. These findings directly link the impaired interaction between SMN and Sm proteins to a defect in snRNP metabolism and to SMA.


Subject(s)
Autoantigens/genetics , Muscular Atrophy, Spinal/genetics , Mutation , Ribonucleoproteins, Small Nuclear/metabolism , Autoantigens/chemistry , Autoantigens/metabolism , Cells, Cultured , Humans , snRNP Core Proteins
9.
EMBO J ; 18(8): 2208-17, 1999 Apr 15.
Article in English | MEDLINE | ID: mdl-10205174

ABSTRACT

Wnt signalling is a key pathway for tissue patterning during animal development. In Drosophila, the Wnt protein Wingless acts to stabilize Armadillo inside cells where it binds to at least two DNA-binding factors which regulate specific target genes. One Armadillo-binding protein in Drosophila is the zinc finger protein Teashirt. Here we show that Wingless signalling promotes the phosphorylation and the nuclear accumulation of Teashirt. This process requires the binding of Teashirt to the C-terminal end of Armadillo. Finally, we present evidence that the serine/threonine kinase Shaggy is associated with Teashirt in a complex. We discuss these results with respect to current models of Armadillo/beta-catenin action for the transmission of the Wingless/Wnt pathway.


Subject(s)
Drosophila Proteins , Drosophila/metabolism , Insect Proteins/metabolism , Proto-Oncogene Proteins/metabolism , Repressor Proteins , Signal Transduction , Trans-Activators , Transcription Factors/metabolism , Amino Acid Sequence , Animals , Armadillo Domain Proteins , Drosophila/genetics , Insect Proteins/chemistry , Molecular Sequence Data , Phosphorylation , Protein Binding , Sequence Homology, Amino Acid , Subcellular Fractions/metabolism , Wnt1 Protein
10.
Cell ; 95(5): 615-24, 1998 Nov 25.
Article in English | MEDLINE | ID: mdl-9845364

ABSTRACT

Spinal muscular atrophy (SMA) is a common motor neuron degenerative disease that results from reduced levels of, or mutations in, the Survival of Motor Neurons (SMN) protein. SMN is found in the cytoplasm and the nucleus where it is concentrated in gems. SMN interacts with spliceosomal snRNP proteins and is critical for snRNP assembly in the cytoplasm. We show that a dominant-negative mutant SMN (SMNdeltaN27) causes a dramatic reorganization of snRNPs in the nucleus. Furthermore, SMNdeltaN27 inhibits pre-mRNA splicing in vitro, while wild-type SMN stimulates splicing. SMN mutants found in SMA patients cannot stimulate splicing. These findings demonstrate that SMN plays a crucial role in the generation of the pre-mRNA splicing machinery and thus in mRNA biogenesis, and they link the function of SMN in this pathway to SMA.


Subject(s)
Muscular Atrophy, Spinal/genetics , Nerve Tissue Proteins/physiology , RNA Precursors/genetics , RNA Splicing , Autoantigens/metabolism , Cyclic AMP Response Element-Binding Protein , HeLa Cells , Humans , Microscopy, Fluorescence , Nerve Tissue Proteins/genetics , Nuclear Proteins/metabolism , Open Reading Frames , Organelles/metabolism , Organelles/ultrastructure , Phosphoproteins/metabolism , RNA-Binding Proteins , Ribonucleoproteins, Small Nuclear/metabolism , SMN Complex Proteins , Spliceosomes/metabolism
11.
Curr Biol ; 8(16): 893-902, 1998.
Article in English | MEDLINE | ID: mdl-9707400

ABSTRACT

BACKGROUND: One function of the Wingless signal cascade is to determine the 'naked' cuticle cell-fate choice instead of the denticled one in Drosophila larvae. Wingless stabilises cytoplasmic Armadillo, which may act in a transcriptional activator complex with the DNA-binding protein T-cell factor (also known as Pangolin). As these components are critical for all Wingless-dependent patterning events, the problem arises as to how specific outputs are achieved. RESULTS: The Teashirt zinc finger protein was found to be necessary for a subset of late Wingless-dependent functions in the embryonic trunk segments where the teashirt gene is expressed. Teashirt was found to be required for the maintenance of the late Wingless signalling target gene wingless but not for an earlier one, engrailed. Armadillo and Teashirt proteins showed similar Wingless-dependent modulation patterns in homologous parts of each trunk segment in embryos, with high levels of nuclear Teashirt and intracellular Armadillo within cells destined to form naked cuticle. We found that Teashirt associates with, and requires, Armadillo in a complex for its function. CONCLUSIONS: Teashirt binds to, and requires, Armadillo for the naked cell-fate choice in the larval trunk. Teashirt is required for trunk segment identity, suggesting that Teashirt provides a region-specific output to Armadillo activity. Further modulation of Wingless is achieved in homologous parts of each trunk segment where Wingless and Teashirt are especially active. Our results provide a novel, cell-intrinsic mechanism to explain the modulation of the activity of the Wingless signalling pathway.


Subject(s)
Drosophila Proteins , Drosophila/embryology , Insect Proteins/physiology , Proto-Oncogene Proteins/physiology , Repressor Proteins , Trans-Activators , Transcription Factors/physiology , Animals , Armadillo Domain Proteins , Body Patterning , Drosophila/genetics , Drosophila/growth & development , Embryo, Nonmammalian/cytology , Embryo, Nonmammalian/physiology , Insect Hormones/physiology , Insect Proteins/genetics , Larva , Phenotype , Proto-Oncogene Proteins/genetics , Signal Transduction , Transcription Factors/genetics , Wnt1 Protein
12.
Mech Dev ; 68(1-2): 157-72, 1997 Nov.
Article in English | MEDLINE | ID: mdl-9431813

ABSTRACT

The Drosophila melanogaster gene teashirt (tsh) is essential for segment identity of the embryonic thorax and abdomen. A deletion 3' to the tsh transcription unit causes the loss of tsh early expression in the even-numbered parasegments, and the corresponding larval cuticular patterns are disrupted. tsh function in the odd-numbered parasegments in these mutants is normal by both criteria. The in vivo activities of genomic fragments from the deleted region were tested in transgenic embryos. A 2.0 kb enhancer from the 3' region acts mainly in the even-numbered parasegments and is dependent on fushi tarazu (ftz) activity, which encodes a homeodomain protein required for the development of even-numbered parasegments. Ftz protein binds in vitro to four distinct sequences in a 220 bp sub-fragment; these and neighboring sequences are conserved in the equivalent enhancer isolated from Drosophila virilis. Tsh protein produced under the control of the 220 bp enhancer partially rescues a null tsh mutation, with its strongest effect in the even-numbered parasegments. Mutation of the Ftz binding sites partially abrogates the capacity for rescue. These results suggest a composite mechanism for regulation of tsh, with different activators such as ftz contributing to the overall pattern of expression of this key regulator.


Subject(s)
Drosophila Proteins , Drosophila/genetics , Homeodomain Proteins/genetics , Repressor Proteins , Transcription Factors/genetics , Transcription, Genetic , Animals , Base Sequence , Binding Sites , Drosophila/embryology , Embryo, Nonmammalian , Enhancer Elements, Genetic , Fushi Tarazu Transcription Factors , Gene Expression Regulation, Developmental , Homeodomain Proteins/metabolism , Molecular Sequence Data , Mutation , Regulatory Sequences, Nucleic Acid , Sequence Homology, Nucleic Acid , Transcription Factors/metabolism
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