ABSTRACT
The chemical composition of the essential oils obtained from the aerial parts of Nepeta camphorata and Nepeta argolica ssp. dirphya were analysed by GC-MS. A total of 52 components were identified and significant differences (qualitative and quantitative) were observed between the two samples. 1,8-Cineol and two nepetalactones were found to be the major components of the oil of N. camphorata and N. argolica ssp. dirphya respectively. The in vitro activity, of the two oils and the three above mentioned isolated compounds, against 25 clinically isolated and commercial strains of Helicobacter pylori was investigated and some activity was found.
Subject(s)
Helicobacter pylori/drug effects , Monoterpenes , Nepeta , Oils, Volatile/pharmacology , Cyclohexanols/chemistry , Cyclohexanols/isolation & purification , Cyclohexanols/pharmacology , Cyclopentane Monoterpenes , Cyclopentanes/chemistry , Cyclopentanes/isolation & purification , Cyclopentanes/pharmacology , Eucalyptol , Gas Chromatography-Mass Spectrometry , Oils, Volatile/analysis , Oils, Volatile/chemistry , Plant Structures/chemistry , Pyrones/chemistry , Pyrones/isolation & purification , Pyrones/pharmacology , Terpenes/chemistry , Terpenes/isolation & purification , Terpenes/pharmacologyABSTRACT
The synthesis and antimicrobial activity of ten labdane-type diterpenes derived from ent-3-beta-hydroxy-13-epi-manoyl oxide (ribenol) is reported. The chloroethyl carbamidic ester 9 showed the strongest antimicrobial activity against all the tested gram (+), gram (-) bacteria and pathogenic fungi. Moreover, the glycoside 11 exhibited an interesting activity against the three tested fungi.
Subject(s)
Anti-Bacterial Agents/chemical synthesis , Anti-Bacterial Agents/pharmacology , Terpenes/chemical synthesis , Terpenes/pharmacology , Anhydrides , Carboxylic Acids/chemical synthesis , Carboxylic Acids/pharmacology , Klebsiella pneumoniae/drug effects , Magnetic Resonance Spectroscopy , Microbial Sensitivity Tests , Pseudomonas aeruginosa/drug effects , Staphylococcus/drug effectsABSTRACT
The new flavonoid glycoside stachyspinoside (1), and the three iridoids, 7-O-acetyl-8-epi-loganic acid (2), ajugol (3) and harpagide (4) were isolated from Stachys spinosa. The structures of these compounds were established on the basis of mass spectrometry (ESMS and tandem MS), one- and two-dimensional nuclear magnetic resonance experiments (COSY, COSY LR, HMQC, TOCSY and HMBC) as well as simple chemical derivatization.