Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 6 de 6
Filter
Add more filters










Database
Language
Publication year range
1.
Mediators Inflamm ; 2015: 628340, 2015.
Article in English | MEDLINE | ID: mdl-26457007

ABSTRACT

While it has long been established that the chemokine receptor CCR9 and its ligand CCL25 are essential for the movement of leukocytes into the small intestine and the development of small-intestinal inflammation, the role of this chemokine-receptor pair in colonic inflammation is not clear. Toward this end, we compared colonic CCL25 protein levels in healthy individuals to those in patients with ulcerative colitis. In addition, we determined the effect of CCR9 pharmacological inhibition in the mdr1a(-/-) mouse model of ulcerative colitis. Colon samples from patients with ulcerative colitis had significantly higher levels of CCL25 protein compared to healthy controls, a finding mirrored in the mdr1a(-/-) mice. In the mdr1a(-/-) mice, CCR9 antagonists significantly decreased the extent of wasting and colonic remodeling and reduced the levels of inflammatory cytokines in the colon. These findings indicate that the CCR9:CCL25 pair plays a causative role in ulcerative colitis and suggest that CCR9 antagonists will provide a therapeutic benefit in patients with colonic inflammation.


Subject(s)
Colitis, Ulcerative/drug therapy , Colitis, Ulcerative/metabolism , Receptors, CCR/antagonists & inhibitors , Receptors, CCR/metabolism , ATP Binding Cassette Transporter, Subfamily B/genetics , ATP Binding Cassette Transporter, Subfamily B/metabolism , Animals , Chemokines, CC/genetics , Chemokines, CC/metabolism , Colitis, Ulcerative/genetics , Female , Humans , In Vitro Techniques , Mice , Mice, Knockout , Sulfonamides/therapeutic use
2.
Arthritis Res Ther ; 16(5): 445, 2014 Sep 24.
Article in English | MEDLINE | ID: mdl-25248373

ABSTRACT

INTRODUCTION: Biological drugs are effective in patients with rheumatoid arthritis (RA), but increase severe infections. The CC chemokine receptor (CCR) 9 antagonist was effective for Crohn's disease without critical adverse effects including infections in clinical trials. The present study was carried out to explore the pathogenic roles of chemokine (C-C motif) ligand (CCL) 25 and its receptor, CCR9, in autoimmune arthritis and to study if the CCR9 antagonist could be a new treatment for RA. METHODS: CCL25 and CCR9 expression was examined with immunohistochemistry and Western blotting. Concentration of interleukin (IL)-6, matrix metalloproteinase (MMP)-3 and tumor necrosis factor (TNF)-α was measured with enzyme-linked immunosorbent assays. Effects of abrogating CCR9 on collagen-induced arthritis (CIA) was evaluated using CCR9-deficient mice or the CCR9 antagonist, CCX8037. Fluorescence labeled-CD11b+ splenocytes from CIA mice were transferred to recipient CIA mice and those infiltrating into the synovial tissues of the recipient mice were counted. RESULTS: CCL25 and CCR9 proteins were found in the RA synovial tissues. CCR9 was expressed on macrophages, fibroblast-like synoviocytes (FLS) and dendritic cells in the synovial tissues. Stimulation with CCL25 increased IL-6 and MMP-3 production from RA FLS, and IL-6 and TNF-α production from peripheral blood monocytes. CIA was suppressed in CCR9-deficient mice. CCX8037 also inhibited CIA and the migration of transferred CD11b+ splenocytes into the synovial tissues. CONCLUSIONS: The interaction between CCL25 and CCR9 may play important roles in cell infiltration into the RA synovial tissues and inflammatory mediator production. Blocking CCL25 or CCR9 may represent a novel safe therapy for RA.


Subject(s)
Arthritis, Experimental/prevention & control , Cell Movement/drug effects , Receptors, CCR/antagonists & inhibitors , Receptors, CCR/metabolism , Small Molecule Libraries/pharmacology , Adoptive Transfer , Animals , Arthritis, Experimental/genetics , Arthritis, Experimental/metabolism , Blotting, Western , Cells, Cultured , Chemokines, CC/metabolism , Chemokines, CC/pharmacology , Fibroblasts/drug effects , Fibroblasts/metabolism , Humans , Immunohistochemistry , Interleukin-6/metabolism , Matrix Metalloproteinase 3/metabolism , Mice, Inbred C57BL , Mice, Inbred DBA , Mice, Knockout , Receptors, CCR/genetics , Spleen/cytology , Synovial Membrane/cytology , Synovial Membrane/metabolism , Tumor Necrosis Factor-alpha/metabolism
3.
PLoS One ; 9(7): e104877, 2014.
Article in English | MEDLINE | ID: mdl-25127029

ABSTRACT

Stromal cells such as myofibroblasts influence tumor progression. The mechanisms are unclear but may involve effects on both tumor cells and recruitment of bone marrow-derived mesenchymal stromal cells (MSCs) which then colonize tumors. Using iTRAQ and LC-MS/MS we identified the adipokine, chemerin, as overexpressed in esophageal squamous cancer associated myofibroblasts (CAMs) compared with adjacent tissue myofibroblasts (ATMs). The chemerin receptor, ChemR23, is expressed by MSCs. Conditioned media (CM) from CAMs significantly increased MSC cell migration compared to ATM-CM; the action of CAM-CM was significantly reduced by chemerin-neutralising antibody, pretreatment of CAMs with chemerin siRNA, pretreatment of MSCs with ChemR23 siRNA, and by a ChemR23 receptor antagonist, CCX832. Stimulation of MSCs by chemerin increased phosphorylation of p42/44, p38 and JNK-II kinases and inhibitors of these kinases and PKC reversed chemerin-stimulated MSC migration. Chemerin stimulation of MSCs also induced expression and secretion of macrophage inhibitory factor (MIF) that tended to restrict migratory responses to low concentrations of chemerin but not higher concentrations. In a xenograft model consisting of OE21 esophageal cancer cells and CAMs, homing of MSCs administered i.v. was inhibited by CCX832. Thus, chemerin secreted from esophageal cancer myofibroblasts is a potential chemoattractant for MSCs and its inhibition may delay tumor progression.


Subject(s)
Chemokines/metabolism , Esophageal Neoplasms/metabolism , Intercellular Signaling Peptides and Proteins/metabolism , Mesenchymal Stem Cells/physiology , Myofibroblasts/metabolism , Animals , Cell Line, Tumor , Chemotaxis , Esophageal Neoplasms/pathology , Humans , Intramolecular Oxidoreductases/metabolism , Macrophage Migration-Inhibitory Factors/metabolism , Matrix Metalloproteinase 2/metabolism , Mice, Inbred BALB C , Mice, Nude , Mitogen-Activated Protein Kinases/metabolism , Neoplasm Transplantation , Protein Kinase C/metabolism , Receptors, Chemokine/metabolism , Transendothelial and Transepithelial Migration
4.
Arterioscler Thromb Vasc Biol ; 33(6): 1320-8, 2013 Jun.
Article in English | MEDLINE | ID: mdl-23559624

ABSTRACT

OBJECTIVE: Obesity and hypertension are comorbid in epidemic proportion, yet their biological connection is largely a mystery. The peptide chemerin is a candidate for connecting fat deposits around the blood vessel (perivascular adipose tissue) to arterial contraction. We presently tested the hypothesis that chemerin is expressed in perivascular adipose tissue and is vasoactive, supporting the existence of a chemerin axis in the vasculature. APPROACH AND RESULTS: Real-time polymerase chain reaction, immunohistochemistry, and Western analyses supported the synthesis and expression of chemerin in perivascular adipose tissue, whereas the primary chemerin receptor ChemR23 was expressed both in the tunica media and endothelial layer. The ChemR23 agonist chemerin-9 caused receptor, concentration-dependent contraction in the isolated rat thoracic aorta, superior mesenteric artery, and mesenteric resistance artery, and contraction was significantly amplified (more than 100%) when nitric oxide synthase was inhibited and the endothelial cell mechanically removed or tone was placed on the arteries. The novel ChemR23 antagonist CCX832 inhibited phenylephrine-induced and prostaglandin F2α-induced contraction (+perivascular adipose tissue), suggesting that endogenous chemerin contributes to contraction. Arteries from animals with dysfunctional endothelium (obese or hypertensive) demonstrated a pronounced contraction to chemerin-9. Finally, mesenteric arteries from obese humans demonstrate amplified contraction to chemerin-9. CONCLUSIONS: These data support a new role for chemerin as an endogenous vasoconstrictor that operates through a receptor typically attributed to function only in immune cells.


Subject(s)
Adipokines/metabolism , Adipose Tissue/metabolism , Aorta, Thoracic/metabolism , Chemokines/metabolism , Mesenteric Arteries/metabolism , Muscle, Smooth, Vascular/physiology , Vasoconstriction/physiology , Adipose Tissue/drug effects , Angiotensin II/pharmacology , Animals , Aorta, Thoracic/drug effects , Blotting, Western , Disease Models, Animal , Gene Expression Regulation , Humans , Hypertension/metabolism , Immunochemistry , Intercellular Signaling Peptides and Proteins , Mesenteric Arteries/drug effects , Obesity/metabolism , Phenylephrine/pharmacology , Rats , Real-Time Polymerase Chain Reaction , Sensitivity and Specificity
5.
PLoS One ; 7(11): e50498, 2012.
Article in English | MEDLINE | ID: mdl-23209760

ABSTRACT

A goal for developers of immunomodulatory drugs has long been a systemically administered small molecule that can selectively inhibit inflammation in specific tissues. The chemokine receptor CCR9 is an attractive target for this approach, as entry of T cells into the small intestine from blood requires interaction between CCR9 and its ligand CCL25. We have tested the ability of a small molecule CCR9 antagonist, CCX8037, to inhibit antigen-mediated T cell accumulation in the intestine. This compound prevented accumulation of gut-imprinted antigen-specific CD8 T cells within epithelium of the small intestine. Interestingly, the antagonist did not affect the robust generation of gut-imprinted CD8 T cells within mesenteric lymph nodes. To distinguish "gut-selective" from "general" T cell inhibition, we tested the drug's ability to influence accumulation of T cells within skin, a tissue in which CCR9 plays no known role, and we found no appreciable effect. This study demonstrates the feasibility of creating systemically-administered pharmaceuticals capable of tissue-selective immune modulation. This proof of concept is of utmost importance for designing effective treatments against various autoimmune disorders localized to a specific tissue.


Subject(s)
Immunologic Factors/pharmacology , Lymphocytes/metabolism , Receptors, CCR/antagonists & inhibitors , Animals , CD8-Positive T-Lymphocytes/drug effects , Cell Line , Cells, Cultured , Chemokines, CC/metabolism , Female , Flow Cytometry , Humans , Lymphocytes/drug effects , Male , Mice
6.
Bioorg Med Chem ; 14(13): 4552-67, 2006 Jul 01.
Article in English | MEDLINE | ID: mdl-16524737

ABSTRACT

A series of analogs of the potent HIV-1 integrase (HIV IN) inhibitor chicoric acid (CA) was designed with the intention of ameliorating some of the parent natural product's undesirable properties, in particular its toxicity, instability, and poor membrane permeability. More than 70 analogs were synthesized and assayed for three types of activity: (1) the ability to inhibit 3'-end processing and strand transfer reactions using recombinant HIV IN in vitro, (2) toxicity against the CD4+ lymphoblastoid cell line, MT2, and (3) anti-HIV activity against HIV(LAI). CA analogs lacking one of the carboxyl groups of CA and with 3,4,5-trihydroxycinnamoyl sidechains in place of the caffeoyl group of CA exhibited the most potent inhibition of HIV replication and end-processing activity. Galloyl-substituted derivatives also displayed very potent in vitro and in vivo activities, in most cases exceeding the inhibitory effects of CA itself. Conversely, analogous monocarboxy caffeoyl analogs exhibited only modest inhibition, while the corresponding 3,4-dihydroxybenzoyl-substituted compounds were devoid of activity.


Subject(s)
Anti-HIV Agents/chemistry , Caffeic Acids/chemistry , Drug Design , HIV Integrase Inhibitors/chemistry , Succinates/chemistry , Anti-HIV Agents/chemical synthesis , Anti-HIV Agents/pharmacology , CD4-Positive T-Lymphocytes/drug effects , Cell Line, Tumor , Cell Membrane , HIV/drug effects , HIV Integrase Inhibitors/chemical synthesis , HIV Integrase Inhibitors/pharmacology , Humans , Recombination, Genetic/drug effects
SELECTION OF CITATIONS
SEARCH DETAIL
...