ABSTRACT
Graphitic pencil leads (PLs) are inexpensive writing accessories, readily available in stationery shops. Because the round filaments have high conductivity, they are excellent candidates for sustainable electroanalytical sensor fabrication. Here, we show that dip-coated carbon nanotube (CNT) surface deposits can stably enhance the faradaic redox response of cylindrical pencil lead electrodes (PLEs), with just ten simple sequential immersions of assembled PLEs in an aqueous suspension of CNTs producing significant improvement in their analytical performance. Cyclic (CV) and differential pulse (DPV) voltammetry of ferricyanide with unmodified and CNT-modified PLEs confirmed the reproducibility of the modification procedure and the reliability of the extent of signal amplification, as well as the stability of the response. A series of DPV tests with drugs, an environmental pollutant, an enzyme-substrate redox label and an industrial chemical proved the practical applicability of the proposed CNT-PLEs. Based on their observed properties, PLEs with dip-coated CNT deposits are suggested as cost-effective tools for advanced electroanalysis and as green platforms for enzyme biosensor construction.
ABSTRACT
Osteoblast-like cells and human mesenchymal stem cells (hMSCs) are frequently employed as osteoprogenitor cell models for evaluating novel biomaterials in bone healing and tissue engineering. In this study, the characterization of UE7T-13 hMSCs and MG-63 human osteoblast-like cells was examined. Both cells can undergo osteogenesis and produce calcium extracellular matrix; however, calcium nodules produced by MG-63 lacked a central mass and appeared flatter than UE7T-13. The absence of growing calcium nodules in MG-63 was discovered by SEM-EDX to be associated with the formation of alternating layers of cells and calcium extracellular matrix. The nanostructure and composition analysis showed that UE7T-13 had a finer nanostructure of calcium nodules with a higher calcium/phosphate ratio than MG-63. Both cells expressed high intrinsic levels of collagen type I alpha 1 chain, while only UE7T-13 expressed high levels of alkaline phosphatase, biomineralization associated (ALPL). High ALP activity in UE7T-13 was not further enhanced by osteogenic induction, but in MG-63, low intrinsic ALP activity was greatly induced by osteogenic induction. These findings highlight the differences between the two immortal osteoprogenitor cell lines, along with some technical notes that should be considered while selecting and interpreting the pertinent in vitro model.
