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Introduction Periodontitis, a persistent inflammatory condition, impacts the tissues supporting teeth. Beyond mechanically eradicating the biofilm, additional host-modulating agents can aid in the treatment of periodontitis. Among these, gels are a very popular choice for use in the field of dentistry as these systems boast high biocompatibility and bioadhesiveness. These qualities make them easily administered and fabricated. They are typically placed into the periodontal site via wide-port needle syringes. Many investigations have demonstrated that hydrogels possess the ability for controlled drug release and aid in periodontal wound healing. Hence, this study aimed to develop a ferulic acid hydrogel and assess its effectiveness for managing periodontitis. Materials and methods Ferulic acid hydrogel was prepared followed by haemolysis assay and biocompatibility assay. After the in vitro analysis, a clinical trial was conducted: 20 patients were divided into Group A (comprising patients in whom scaling and root planing (SRP) was done) and Group B (comprising patients in whom SRP along with hydrogel application was done). Each patient's pocket depth (PD), clinical attachment loss (CAL), gingival index (GI), and plaque index (PI) were recorded at baseline and at three months. Intergroup and intragroup comparisons of the parameters were made. Results Ferulic acid hydrogels exhibit a minimal ratio of red blood cell destruction, indicating their low haemolytic activity. Beyond 94 hours, ferulic acid hydrogel demonstrates minimal toxicity towards human fibroblasts, suggesting it has good biocompatibility. When clinical parameters were compared after three months of treatment with SRP alone, significant reductions were observed in all parameters. However, when hydrogel application was done along with SRP, greater reduction was seen in terms of all clinical parameters indicating the efficacy of the ferulic acid hydrogel as an adjunct. Conclusion Ferulic acid has distinct haemolytic activity as well as good biocompatibility. Its use also led to a considerable reduction in all clinical parameters, necessitating its role as a local drug delivery agent in the treatment of periodontitis.
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BACKGROUND: Chronic periodontitis is a multifactorial inflammatory condition influenced by genetic factors. Matrix metalloproteinase (MMP)-13, serving as a crucial enzyme involved in extracellular matrix remodeling, is associated with the degradation of periodontal tissues. Therefore, this study assesses the genetic link between the MMP-13 (rs2252070) genetic variation and chronic periodontitis in a Southern Indian demographic. METHODOLOGY: The study was conducted at Saveetha Dental College in Chennai, India. It involved a total of 100 subjects, 50 individuals affected with periodontitis (classified as stage II and above, American Association of Periodontology 2018 criteria) and 50 individuals who were periodontally healthy or were diagnosed as having mild gingivitis. We isolated DNA from the blood samples obtained from the participants. Specific primers that flank the BsrI region of the MMP-13 receptor gene were used in the process of DNA amplification. Subsequently, a restriction fragment length analysis using the BsrI enzyme was carried out for genotyping of the amplicon. Based on the restriction fragment length polymorphism pattern, we obtained certain genotypes. These were further recorded and followed by statistical analysis. We conducted a chi-square test to draw a comparison in terms of their genotype and allele frequencies. We calculated the odds ratio, along with 95% confidence intervals. RESULTS: The frequency of genotypes and distribution of MMP-13 polymorphism did not exhibit a statistically significant difference at χ2 degrees of freedom (P = 0.913). We inferred from our study that there was no significant difference between the groups concerning homozygous and heterozygous mutant genotypes (AA vs. AG + GG), with a P-value of 0.6871. The observed frequencies of GG (47% vs. 43%) and AG+AA (41% vs. 42%) genotypes did not indicate a significant difference between the groups. Similarly, there was no noteworthy distinction between the A allele (62% vs. 65%) and G allele (38% vs. 35%) in the case and control groups. CONCLUSION: The findings of the study reveal that there is no correlation between MMP-13 (rs2252070) gene polymorphism and periodontitis.
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Introduction Nanotechnology plays a significant role in the biomedical and dental fields, offering numerous benefits to humans. Particularly, nanoparticles synthesised through green methods involving herbal formulations present promising advantages. Zinc oxide nanoparticles (ZnONPs) demonstrate strong antibacterial properties. Utilising treatments incorporating chamomile tea and green tea may potentially reduce toxicity while enhancing antibacterial effectiveness against oral infections. This study aimed to develop a mouthwash containing ZnONPs, followed by an evaluation of both its cytotoxicity and antibacterial effectiveness. Materials and methods This study was conducted at Saveetha Dental College, Saveetha Institute of Medical and Technical Science, Chennai, India. In the synthesis of ZnONPs, a formulation consisting of chamomile tea and green tea was employed. Subsequently, these synthesised nanoparticles were used in the preparation of mouthwash. An antimicrobial test of the produced ZnONPs was carried out using the agar well diffusion technique for oral pathogens. For analysis of cytotoxicity, brine shrimps were used in an assay, and comparisons were made with a commercially available mouthwash. Results The antimicrobial properties were assessed, and the formulated mouthwash demonstrated a zone of inhibition of Staphylococcus aureus (20 mm), Enterococcus faecalis (11 mm), Streptococcus mutans (15 mm) and Candida albicans (13 mm), when the agar well diffusion assay was carried out. Furthermore, the formulated mouthwash exhibited lower cytotoxicity compared to the commercially available mouthwash when cytotoxicity was checked in brine shrimps. Conclusion In our study, the ZnONP synthesis with chamomile tea and green tea showed notable antibacterial and antifungal effects. In addition, lower toxicity was observed compared to the commercially available mouthwash. These findings suggest that mouthwash formulated with green-synthesis ZnONPs could serve as a viable alternative to synthetic mouthwash options. As a result, it is suggested that ZnONPs could be employed in mouthwash formulations at concentrations of 40 µL.
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Background and introduction Periodontal disease is one of the most prevalent chronic conditions that affects the oral cavity. Identifying and predicting biomarkers is essential for the prevention of high-morbidity oral diseases. The genomic interaction network identifies common hub genes involved in crucial protein formation in periodontal inflammation. Diabetes mellitus is a metabolic disorder that has a double-edged sword relationship with periodontitis. Chloride intracellular channel 1 (CLIC1) was identified as a hub gene linking the pathogenesis of periodontitis and diabetes mellitus using a bioinformatic tool. Therefore, this current study aimed to assess the concentration of the pro-inflammatory biomarker CLIC1 in saliva among individuals with periodontal health and those with periodontal disease linked to diabetes mellitus. Materials and methods Differentially expressed genes (DEGs) in periodontitis were identified using datasets retrieved from the Gene Expression Omnibus (GEO) database. DEGs were combined to build the network, and GeneMANIA was used to find and rank the interconnecting genes. CLIC1 was identified as the hub gene, and clinical validation was done using patient samples. The study involved 30 participants. Based on clinical and radiographic periodontal findings, they were split into three groups: healthy (group 1, n=10), with periodontitis but no diabetes mellitus (group 2, n=10), and with periodontitis and diabetes mellitus (group 3, n=10). The collection of saliva samples, followed by quantifying these samples, was performed using an enzyme-linked immunosorbent assay (ELISA). Results From network graph analysis, it was discovered that CLIC1 functions as a hub gene in the majority of toll-like receptor pathways. The mean concentration of CLIC1 in saliva increased consistently as the disease was observed in periodontitis patients and periodontitis patients with diabetes mellitus. Conclusion CLIC1 concentrations were positively correlated with periodontitis in individuals with diabetes. Therefore, CLIC1 could be a diagnostic biomarker for patients with periodontitis. However, large-scale studies are needed to confirm more positive associations.
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Background The primary cause of adult tooth loss is commonly attributed to periodontal disease, a condition that weakens the supportive structures around the teeth. In addressing periodontal diseases, surgeons often employ the guided tissue regeneration (GTR) technique, which involves the use of a barrier membrane. Aim The aim of the present study is to assess the composition and mechanical strength of chitosan and eggshell membrane. This research was conducted to provide insights into their potential application in facilitating tissue regeneration. Materials and procedures Chitosan and eggshell membrane were combined to create the membrane. Scanning electron microscopy (SEM) using FEI Quanta FEG 650 SEM (JSM IT-800, JEOL Ltd., Akishima, Tokyo, Japan) was carried out, and mechanical properties were used to measure the parameters of membrane characterization. Results In the dry condition, the membrane's tensile strength was 0.30 MPa and its elongation at break was 8.2%. In the wet condition, the membrane's tensile strength was 0.13 MPa and its elongation at break was 22.6%. The SEM results depicted membrane surface with pore sizes ranging from 16 to 100 meters, and the result obtained from membrane porosity test was 31.2%. Conclusion The chitosan-eggshell membrane exhibited a fibrous surface with a desirable pore size for use as a GTR membrane, but it has low mechanical strength.
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Background An important field of study in contemporary material science is the synthesis of metallic nanoparticles. The wide range of uses for zinc nanoparticles in industries like diagnosis and antimicrobial catalysis has sparked particular interest in them. Plant extracts are used to synthesize zinc nanoparticles, opening up a wide range of potential applications. Hence, the current study aims to demonstrate the anti-inflammatory and antioxidant activity of zinc oxide nanoparticles mediated by green tea and chamomile tea combination. Materials and methods Leaves of green tea and chamomile tea were combined in a ratio of 1 gram each. To make a 1-molar solution of the extract, the weighted extracts were thoroughly mixed with 100 ml of distilled water in conical flasks. To synthesize nanoparticles, a magnetic stirrer and an orbital shaker were used alternately with an extract of chamomile and green tea, 0.016 g of zinc oxide, and 90 ml of distilled water at 900 rpm. By using an albumin denaturation assay, the synthesized nanoparticles' anti-inflammatory activity was assessed. Bovine serum albumin (BSA) and egg albumin (EA) were the reagents used in the assay. The antioxidant activity of zinc oxide nanoparticles, which is mediated by chamomile and green tea, was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and H2O2 (hydrogen peroxide) radical scavenging assays. An independent sample t-test was done to compare the anti-inflammatory and antioxidant potentials of zinc oxide nanoparticles mediated by green tea and chamomile tea combination and control using SPSS Statistics version 22.0 software (IBM Corp. Released 2013. IBM SPSS Statistics for Windows, Version 22.0. Armonk, NY: IBM Corp.), and any p-value less than 0.05 was considered statistically significant. Results In this study, the anti-inflammatory activity and antioxidant activity were assessed at variable concentrations of the reaction mixture. The combination of chamomile and green tea extracts mediated by zinc oxide nanoparticles at 50µl concentration showed the maximum anti-inflammatory activity and antioxidant activity at 87% inhibition, respectively. Conclusion Both assays successfully demonstrated better anti-inflammatory and antioxidant activity of zinc oxide nanoparticles mediated by chamomile and green tea combination when compared to control and, therefore, could be evaluated as a potential therapeutic agent.
