ABSTRACT
Corneal perforation and withdrawal of aqueous humor (paracentesis) represents a practical means for producing controlled trauma to the eye. The sequence of events triggered by paracentesis has been carefully studied by in vivo and in vitro microscopy and other means. These observations have proven especially helpful in elucidating the physiology and pharmacology of ocular trauma. Scanning electron microscopy of the ciliary processes of the rabbit has proven a sensitive indicator of the response to prostaglandin, a mediator of the paracentesis reaction. We used SEM to invetigate changes in the ciliary processes induced by paracentesis. We also examined the opposite eye for a consensual response. The effect of prior treatment with aspirin or nerve block was assessed in both the traumatized eye and the fellow eye. In an untreated animal, a consensual response to trauma was present and demonstrable by scanning electron microscopy. The direct response was more effectively blocked by aspirin than by local anesthesia. Both pretreatments were effective in reducing the consensual reaction.
Subject(s)
Ciliary Body/ultrastructure , Eye Injuries , Animals , Aspirin/pharmacology , Ciliary Body/pathology , Disease Models, Animal , Female , Functional Laterality , Male , Microscopy, Electron, Scanning , Prostaglandins/physiology , RabbitsABSTRACT
We examined trabeculectomy specimens obtained at the time of surgery for inadequately controlled open-angle glaucoma by scanning electron microscopy. Of the 50 specimens ten included a broad area of the trabecular meshwork. In these ten specimens the meshwork was obscured by material. We believe the degree of occlusion was significant in these specimens, which would lead to obstruction of aqueous outflow. No similar material was found in control specimens from persons of similar age.
