Microplastic Abundance and Sources in Surface Water Samples of the Vaal River, South Africa.

Microplastics (MPs) have emerged as a global environmental concern due to their persistent nature. In South Africa, microplastic research has primarily focused on marine systems. However, recent years have seen a shift in focus to studying MPs in South African freshwaters. In this study, MPs with a minimum size of 0.055 mm in surface water of the Vaal River, South Africa, were reported. MPs were 100% prevalent, with a mean numerical abundance of 0.68 ± 0.64 particles/m3. Small-sized MPs of < 1 mm accounted for the largest proportion. MPs were chemically identified as high-density polyethylene, low-density polyethylene, and polypropylene according to their Raman spectra. The prevalence of fragments (41.6%) and fibers (38.5%) over pellets (8.1%) indicates that microplastics are from secondary sources. The prevalence of polyethylene and polypropylene is consistent with microplastics being from secondary sources. These polymers are commonly used in single-use plastics, packing bags, textiles, and containers. These characteristics are of great concern due to their implications on the bioavailability and toxicological impacts of MPs. Consequently, these properties may pose more hazards to aquatic biota inhabiting the Vaal River.

First biomonitoring of microplastic pollution in the Vaal river using Carp fish (Cyprinus carpio) "as a bio-indicator".

Fish inhabiting freshwater environments are susceptible to the ingestion of microplastics (MPs). Knowledge regarding MPs in freshwater fish in South Africa is very limited. In this study, the uptake of MPs by common carp (Cyprinus carpio) in the Vaal River in South Africa was assessed. MPs were detected in all of the twenty-six fish examined, 682 particles of MPs were recovered from the gastrointestinal tracts of the fish with an average of 26.23 ± 12.57 particles/fish, and an average abundance of 41.18 ± 52.81 particles/kg. The examination of the physical properties of MPs revealed a predominance on fibers (69%), small-sized particles of less than 0.5 mm (48%), as well as prevelance of coloured MPs (94%), mostly green, blue, and black. Using Raman Spectroscopy, the following plastic polymers were identified: high density polyethylene (HDPE), low density polyethylene (LDPE), polypropylene (PP), polyethylene terephthalate (PET), and polytetrafluoroethylene (PTFE). To the best of our knowledge, this study, is the first to report MPs uptake by freshwater biota in the Vaal River using common carp as a target organism. It provided evidence of MP contamination in the Vaal.

Lost to follow up rate in the first year of ART in adults initiated in a universal test and treat programme: a retrospective cohort study in Ekurhuleni District, South Africa.

INTRODUCTION: South Africa adopted and implemented the Universal Test and Treat (UTT) strategy for HIV since 2016. However, the care outcomes for patients initiated antiretroviral therapy (ART) through the UTT strategy have not been established. We determined the rate of lost to follow up (LTFU) and associated factors in patients who were initiated on ART through the UTT and the pre-ART strategy at 12 months post ART initiation. METHODS: this retrospective study analyzed the records of a cohort of patients at 12 months post the initiation of ART. We extracted data from the TIER.Net electronic database of selected facilities in a sub-district in Gauteng Province, South Africa. Factors associated with LFTU at 12 months of ART were assessed and logistic regression performed to identify predictors of LFTU. RESULTS: records of 367 patients were evaluated, and 54% were initiated ART through the UTT strategy. The mean age was 36.3 years, mean CD4 cell count at ART initiation was 341 cells/mm3, and 25% were initiated at CD4 cell count above 500 cells/mm3. LTFU at 12 months was 28%, 50% were LFTU within six months, and 28% within three months of ART. LFTU in the UTT cohort was higher than in the pre-ART cohort, patients initiated through UTT were twice more likely to be LTFU (AOR = 1.84, CI: 1.13-3.00) than pre-ART patients. CONCLUSION: the rate of LTFU at 12 months of ART was 28%, which indicate that the retention in care rate (60%) falls far short of the triple 90 targets required for viral suppression.