ABSTRACT
In the present study, seven fungal isolates from effluent treatment plants were screened for the production of prebiotic fructooligosaccharide synthesizing enzymes with the highest activity of fructofuranosidase (17.52 U/mL) and fructosyl transferase (18.92 U/mL) in strain HKF-74. Mining of genome sequence of strain revealed the annotation of genes providing multiple carbohydrate metabolizing capacities, such as amylases (AMY1), beta-galactosidase (BGAL), beta-xylosidase (Xyl), ß-fructofuranosidase (ScrB), fructosyltransferase (FTF), and maltose hydrolases (malH). The annotated genes were further supported by ß-galactosidase (15.90 U/mL), xylanase (17.91 U/mL), and α-amylase (14.05 U/mL) activities for synthesis of galactooligosaccharides, xylooligosaccarides, and maltooligosaccharides, respectively. In addition to genes encoding prebiotic synthesizing enzymes, four biosynthetic gene clusters (BGCs) including Type I polyketide synthase (PKS), non-ribosomal peptide synthetase (NRPS), NRPS-like, and terpene were also predicted in strain HKF-74. This was significant considering their potential role in pharmaceutical and therapeutic applications as well as in virulence. Accurate taxonomic assignment of strain HKF-74 by in silico genomic comparison indicated its closest identity to type strains Fusarium verticillioides NRRL 20984, and 7600. The average nucleotide identity (ANI) of strain HKF-74 with these strains was 92.5% which was close to the species threshold cut-off value (95-96%) while the DNA-DNA hybridization (DDH) value was 83-84% which was greater than both, species delineating (79-80%), and also sub-species delineating (70%) boundaries. Our findings provide a foundation for further research into the use of Fusarium strains and their prebiotic synthesizing enzymes for the development of novel prebiotic supplements.
ABSTRACT
Prebiotics as a part of dietary nutrition can play a crucial role in structuring the composition and metabolic function of intestinal microbiota and can thus help in managing a clinical scenario by preventing diseases and/or improving health. Among the different prebiotics, non-digestible carbohydrates are molecules that selectively enrich a typical class of bacteria with probiotic potential. This review summarizes the current knowledge about the different aspects of prebiotics, such as its production, characterization and purification by various techniques, and its link to novel product development at an industrial scale for wide-scale use in diverse range of health management applications. Furthermore, the path to effective valorization of agricultural residues in prebiotic production has been elucidated. This review also discusses the recent developments in application of genomic tools in the area of prebiotics for providing new insights into the taxonomic characterization of gut microorganisms, and exploring their functional metabolic pathways for enzyme synthesis. However, the information regarding the cumulative effect of prebiotics with beneficial bacteria, their colonization and its direct influence through altered metabolic profile is still getting established. The future of this area lies in the designing of clinical condition specific functional foods taking into consideration the host genotypes, thus facilitating the creation of balanced and required metabolome and enabling to maintain the healthy status of the host.
ABSTRACT
In the present study, the microbial community residing at different depths of the landfill was characterized to assess their roles in serving as a methane sink. Physico-chemical characterization revealed the characteristic signatures of anaerobic degradation of organic matter in the bottom soil (50-60 cm) and, active process of aerobic denitrification in the top soil (0-10 cm). This was also reflected from the higher abundance of bacterial domain in the top soil metagenome represented by dominant phyla Proteobacteria and Actinobacteria which are prime decomposers of organic matter in landfill soils. The multiple fold higher relative abundances of the two most abundant genera; Streptomyces and Intrasporangium in the top soil depicted greater denitrifying taxa in top soil than the bottom soil. Amongst the aerobic methanotrophs, the genera Methylomonas, Methylococcus, Methylocella, and Methylacidiphilum were abundantly found in the top soil metagenome that were essential for oxidizing methane generated in the landfill. On the other hand, the dominance of archaeal domain represented by Methanosarcina and Methanoculleus in the bottom soil highlighted the complete anaerobic digestion of organic components via acetoclasty, carboxydotrophy, hydrogenotrophy, methylotrophy. Functional characterization revealed a higher abundance of methane monooxygenase gene in the top soil and methyl coenzyme M reductase gene in the bottom soil that correlated with the higher relative abundance of aerobic methanotrophs in the top soil while methane generation being the active process in the highly anaerobic bottom soil in the landfill. The activity dependent abundance of endogenous microbial communities in the different zones of the landfill was further validated by microcosm studies in serum bottles which established the ability of the methanotrophic community for methane metabolism in the top soil and their potential to serve as sink for methane. The study provides a better understanding about the methanotrophs in correlation with their endogenous environment, so that these bacteria can be used in resolving the environmental issues related to methane and nitrogen management at landfill site.
