Chemiluminescent detection of glycocode alterations in hepatic granulomatous lesions of experimental schistosomiasis.

This work describes the glycophenotype evaluation of mice liver granulomatous lesion caused by infection of Schistosoma mansoni by using lectins labeled to acridinium ester (AE). The employed lectins were Concanavalin A (Con A), wheat germ agglutinin (WGA) and Sambucus nigra agglutinin (SNA) that specifically recognize α-D-glucose/mannose, N-acetyl-D-glucosamine and α-NeuNAc-[2→6]-Gal/GalNAc. The chemiluminescence expressed in relative light unit (RLU) obtained from the hepatic granuloma tissues (0.25 cm2) treated with the lectins-AE was compared with control tissues. Con A-AE infected tissues showed higher statistically significant values (1,501,182 ± 163,450 RLU) compared with the control tissue (575,280 ± 97,216 RLU). WGA-AE results also showed higher values (189,654 ± 20,686 RLU) than that found for the controls (82,878 ± 24,411). SNA-AE results did not present statistical difference between granulomatous tissues (198,990 ± 15,131) and controls (167,290 ± 25,194). There is a significant increase in glucose/mannose residues and N-acetyl-D-glucosamine in hepatic granuloma caused by S. mansoni, while the sialic acid remains virtually unchanged. The understanding of schistosome glycophenotype is relevant for the development of new diagnostic methods for schistosomiasis, design of new drug targets and preparation of glycan-based vaccines.

[Distribution of eosinophils at different stages of hepatic granuloma evolution in mice infected with Schistosoma mansoni]. / A distribuição dos eosinófilos nas diferentes fases de evolução do granuloma hepático em camundongos infectados pelo Schistosoma mansoni.

In the present study, the distribution of eosinophils at different stages of the formation of hepatic granuloma in mice infected with Schistosoma mansoni was evaluated. From the results obtained, we suggest a new classification for the evolution of hepatic granuloma in mice, constructed from the phases described by other authors. In each phase, there is a different pattern of eosinophil distribution. In the exudative-necrotic phase, the eosinophils are concentrated in the periphery and center of the granuloma, and are scarce in the necrotic area; in the productive phase, the eosinophils are dispersed throughout the granuloma; and in the cure due to fibrosis phase, the eosinophils are concentrated in the periphery and center of the granuloma. Eosinophils were found in direct contact with the eggs at all stages of evolution of the granuloma. It was concluded that the dynamics of eosinophils have an important role in forming the granulomatous reaction of the host and in resolving the inflammatory process caused by the parasite egg, as well as adding new data regarding hepatic granuloma classification.

A distribuição dos eosinófilos nas diferentes fases de evolução do granuloma hepático em camundongos infectados pelo Schistosoma mansoni / Distribution of eosinophils at different stages of hepatic granuloma evolution in mice infected with Schistosoma mansoni

No presente estudo, avaliou-se a distribuição dos eosinófilos nas diferentes fases da formação do granuloma hepático de camundongos infectados pelo Schistosoma mansoni. A partir dos resultados obtidos sugerimos uma nova classificação para a evolução do granuloma hepático em camundongos montada a partir de fases descritas por outros autores. Em cada fase há um padrão diferente de distribuição dos eosinófilos. Na fase necrótico-exudativa os eosinófilos encontram-se concentrados na periferia e no centro do granuloma e na área de necrose eles são escassos; na "produtiva" os eosinófilos estão ainda distribuídos de maneira difusa por todo o granuloma; na de cura por fibrose se concentram na periferia e no centro do granuloma. Os eosinófilos estavam em contato direto com os ovos em todos os estágios de evolução dos granulomas. Conclui-se então que a dinâmica dos eosinófilos possui papel importante na formação da reação granulomatosa do hospedeiro e resolução do processo inflamatório causado pelo ovo do parasita, além de acrescentar novos dados na classificação dos granulomas hepáticos.

In the present study, the distribution of eosinophils at different stages of the formation of hepatic granuloma in mice infected with Schistosoma mansoni was evaluated. From the results obtained, we suggest a new classification for the evolution of hepatic granuloma in mice, constructed from the phases described by other authors. In each phase, there is a different pattern of eosinophil distribution. In the exudative-necrotic phase, the eosinophils are concentrated in the periphery and center of the granuloma, and are scarce in the necrotic area; in the productive phase, the eosinophils are dispersed throughout the granuloma; and in the cure due to fibrosis phase, the eosinophils are concentrated in the periphery and center of the granuloma. Eosinophils were found in direct contact with the eggs at all stages of evolution of the granuloma. It was concluded that the dynamics of eosinophils have an important role in forming the granulomatous reaction of the host and in resolving the inflammatory process caused by the parasite egg, as well as adding new data regarding hepatic granuloma classification.