In vitro and in silico study of eggplant extract on human cancer cell lines.

The aim of this research was to study the metabolite composition, antioxidative potential and cytotoxic activities of Solanum melongena fruit extracts. Phytochemical analyses of extracts were performed using LC-MS. Phenolic compounds were the major constituents present in the fruit extracts. Free radical scavenging activities were recorded and the highest activities were reported in methanolic extracts using DPPH (103.70 ± 2.75 EC50 µg/mL), ABTS (81.74 ± 3.64 EC50 µg/mL), and FRAP (22.39 ± 1.52 µmol TE/g) assays. Quantification has suggested the presence of delphinidin derivatives, and caffeic acid conjugates as major constituents of fruit extracts. The potential binding of these derivatives with human cell surface receptors was analysed using in silico analysis and validated for cytotoxic and apoptotic effects using in vitro studies on human cancer cell lines. The methanolic extract has shown the highest cytotoxic activity.

Development and quality evaluation of polyphenols enriched black carrot (Daucus carota L.) powder incorporated bread.

Black carrot is a prominent source of polyphenols and the cheapest source of anthocyanins in India. In this study, an attempt has been made to examine the feasibility of black carrot powder as an ingredient in bread. Black carrot bread was prepared by incorporating different concentrations of black carrot powder (BCP) at 2.5, 5.0, 7.5 and 10 %. The developed bread samples were analyzed for physical and textural quality, proximate composition, bioactive compounds, antioxidant properties, sensory characteristics, mineral content and storage quality. The results revealed that loaf volume and specific volume decreased (1995-1254 mL, 5.25-3.28 mL/g) with the incorporation of BCP into bread. Textural analysis revealed that the addition of BCP led to increased hardness in the bread (0.110-12 0.151 N), whereas the resilience (43.64-35.10 %), cohesion and springiness (89.930-13 82.146 %) decreased significantly. The content of bioactive compounds such as total phenols, anthocyanins (29.63-112.68 mg/100 g) and flavonoids increased to exceptionally high levels in BCP-incorporated bread and showed high antioxidant activity. Incorporation of BCP up to 7.5 % showed the most acceptable sensory analysis score (7.85) with a significant increase in dietary fiber (40 %) and total mineral content (50 %), which revealed that black carrot powder could be used up to 7.5 % as an ingredient into bread with high acceptability. The present study revealed significant enhancement in bioactive compounds and mineral content of bread after the incorporation of black carrot powder, which supports its immense potential in preventing hunger and oxidative stress-induced disorders in developing countries.

Utilizing response surface methodology to optimize the extraction and characterization of anthocyanins from Solanum melongena L. fruit.

This research aimed to optimize the extraction of anthocyanin from Solanum melongena L. fruits by studying the effects of extraction time, temperature, pH, and solid-to-liquid ratio using response surface methodology. The Box-Behnken design was used to analyze the anthocyanin concentration under different combinations of these parameters. The optimal conditions for anthocyanin extraction were found to be 7.9 h extraction time, 33.63 °C extraction temperature, 5.39 pH of extraction solvent, and 0.32 solid-liquid ratio. The recovery of 88.12 mg·100 g-1 anthocyanin concentration was achieved with 0.53% relative error compared to the output generated through the chemometric model. Statistical analysis revealed a significant effect of the selected parameters on anthocyanin extraction. The extracted anthocyanin was characterized and found to contain delphinidin. These findings suggest that the optimized method can be used for the efficient extraction and characterization of anthocyanins from Solanum melongena L. fruits.

Evaluation of biochemical composition of hulled and hull-less genotypes of pumpkin seeds grown in subtropical India.

Pumpkin seeds are one of the functional foods with most potential having myriad of uses, and functioning as both edible seeds and oilseeds. Nevertheless, their utilization is restricted to the presence of a thick seed coat (hull) which subjects them to the process of decortication, increasing the farmers' expense as well as limiting their utilization as oilseeds. Therefore, in the present study, characterization of the biochemical composition of the hulled (Punjab Chappan Kadoo-1 abbreviated as PCK-1) and hull-less (PAU Magaz Kadoo-1 abbreviated as PMK-1) genotype of pumpkin seeds was undertaken to assess the nutritional differences and their efficient application; PMK-1 is a new cultivar of pumpkin released by Punjab Agricultural University in 2018. Based on the characterization, the hulled genotype of pumpkin seeds was observed to possess higher content of total soluble proteins (79.62 mg/100 g), total free amino acids (3.48 g/100 g), moisture (6.74%), fibre content (21.1 g/100 g), antioxidant potential (26.15%), polyamines (19.2 mg/100 g), sterols (387.1 mg/100 g), and specific enzymatic activity whereas the hull-less genotype was observed to possess a higher amount of minerals (4.57 g/100 g), tocopherols (15.76 mg/100 g), and oil content (36%) respectively; most of the biochemical parameters do not differ from each other at a greater fold difference except for total free amino acids and fibre, which are nearly four times and three times higher in hulled seeds in comparison to the naked seeds respectively. The two genotypes of seeds do not compete, rather do complement each other in biochemical and nutritional composition.

Role of phenylalanine/tyrosine ammonia lyase and anthocyanidin synthase enzymes for anthocyanin biosynthesis in developing Solanum melongena L. genotypes.

Solanum melongena is a widely consumed vegetable crop comprising health-benefiting phenolic compounds. It has a complex network of biosynthetic enzymes involved in synthesizing nutraceuticals, including anthocyanins. The present study was conducted to investigate the activities of key enzymes involved in biosynthesis and accumulation of anthocyanins in developing genotypes, such as phenylalanine ammonia lyase (PAL), tyrosine ammonia lyase (TAL), and anthocyanidin synthase (ANS). As inadequate information is available in this context, fruit and leaf tissues were analyzed for enzyme activities and anthocyanin accumulation. The study included characterization of extracted anthocyanin followed by expression studies for gateway enzyme (ANS) involved in anthocyanin biosynthesis. Delphinidin was a major anthocyanidin present in fruit tissues (1.46-110.49 mg/100 g) of S. melongena. Anthocyanin accumulation is backed up by the correlation between biochemical analysis and expression studies. The study has shown variation for PAL, TAL and ANS enzymes in different tissues at developmental stages. Enzyme activities had a strong positive correlation with anthocyanin biosynthesis.