ABSTRACT
AIM: Oral cancer ranks in top three of all cancers in India and accounts for over 30% of all cancers reported in the country. The present study aims at an in vivo cytogenetic analysis to assess the applicability of the micronucleus test in diagnosing early detection of dysplastic changes. MATERIALS AND METHODS: Exfoliated scrape smears were collected from 75 patients suffering from premalignant lesions. The wet-fixed smears were stained by adopting Papanicolaou's staining protocol. The analysis of variance (ANOVA) test was used to analyze the data statistically. RESULTS: The results of the above study give a fair assessment of the amount of nuclear alterations seen in individuals exposed to genotoxic agents, such as tobacco, and also to a certain extent, an insight into the expected biological behavior of the lesions present in such individuals. Detection of micronuclei and their assay is an upcoming research domain in the field of cancer detection and therapeutics. These miniature nuclear offshoots if properly identified can turn out to be important biomarkers with huge potential for screening and predicting patients with oral precancers and also can act as risk assessors in patient's ongoing treatment for invasive cancers. CONCLUSION: The frequency of micronucleated cells has been observed to be in increasing order with the increase in the age-groups and from control to precancerous cases significantly in both sexes.
Subject(s)
Mouth Mucosa , Nicotiana , Cytogenetic Analysis , Female , Humans , India , Male , Micronucleus TestsABSTRACT
AIM: To assess the cases of ameloblastoma retrospectively for various parameters. MATERIALS AND METHODS: Ninety-one previously reported cases of ameloblastoma from Government Dental College and Hospitals, Nagpur, were included in this study. Data were collected considering parameters such as age, sex, symptoms, radiographic, histopathology, treatment modalities, and recurrence. Our findings were also compared with world literature. RESULTS: The average age was 32.5 years. Mostly men complained of an asymptomatic swelling and duration of less than one year. Posterior segment and vertical ramus of mandible was the frequently involved site. Radiographically, multilocular appearance was noted more than 60% of the cases. Plexiform and unicystic ameloblastoma occurred frequently. Surgery was the treatment of choice in this review. Recurrence was noted in more than 10% of the cases. CONCLUSION: There are variations in our review in comparison to reported literature.
ABSTRACT
BACKGROUND: Ameloblastoma is a rare, benign tumor of odontogenic epithelium, but with an aggressive clinical behavior. AIM: The present study aims to assess the ultramicroscopic features of the epithelial and connective tissue components of ameloblastoma. MATERIALS AND METHODS: Six cases of ameloblastoma were subjected to electron microscopy. They included three cases of follicular type and three cases of plexiform type. RESULTS: The study reveals that the ameloblastoma contains the full complement of cells normally found during odontogenesis. However, these cells resemble the enamel organ in an undifferentiated stage. CONCLUSION: Ultramicroscopy revealed the presence of different cell types among follicular type. The morphology of plexiform variants and correlation with odontogenesis could be elicited. The electron microscopic differences between follicular and plexiform types could help us in better understanding its pathogenesis.
