ABSTRACT
A novel bacterial strain, designated as LNUB461T, was isolated from soil sample taken from the countryside of Shenyang, Liaoning Province, China. The isolate was a Gram-stain-positive, aerobiotic, motile, endospore-forming and rod-shaped bacterium. The organism grew optimally at 30-33 °C, pH 6.5-7.0 and in the absence of NaCl. Phylogenetic analysis based on the nearly full-length 16S rRNA gene sequence revealed high sequence similarity with Paenibacillus algorifonticola XJ259T (98.5 %), Paenibacillus xinjiangensis B538T (96.8 %), Paenibacillus glycanilyticus DS-1T (96.1 %) and Paenibacillus lupini RLAHU15T (96.1 %). The predominant cellular fatty acid and the only menaquinone were anteiso-C15:0 and MK-7, respectively. The main polar lipids of LNUB461T included phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC) and two unknown amino phospholipids (APL), and the cell-wall peptidoglycan was meso-diaminopimelic acid (A1γ). The DNA G+C content of LNUB461T was 49.1 mol%. The DNA-DNA hybridization value between LNUB461T and the most closely related species (P. algorifonticola) was 41.8 %. On the basis of these data, LNUB461T was classified as representing a novel species of the genus Paenibacillus, for which the name Paenibacillus liaoningensis sp. nov was proposed. The type strain is LNUB461T (=JCM 30712T=CGMCC 1.15101T).
Subject(s)
Paenibacillus/classification , Phylogeny , Soil Microbiology , Aerobiosis , Bacterial Typing Techniques , Base Composition , Cell Wall/chemistry , China , DNA, Bacterial/genetics , Diaminopimelic Acid/chemistry , Fatty Acids/chemistry , Nucleic Acid Hybridization , Paenibacillus/genetics , Paenibacillus/isolation & purification , Peptidoglycan/chemistry , Phospholipids/chemistry , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Vitamin K 2/analogs & derivatives , Vitamin K 2/chemistryABSTRACT
In this paper, a microfabricated inductive coil comprising of 125-turn coil windings and a MnZn-based magnetic core in a volume of 200 mm(3) is presented for the magnetic neural stimulation in a spinal cord. The coil winding with the parallel-linkage design instead of the typical serial-linkage one is proposed not only to provide better design flexibility to the current mode driving circuit but also to simplify the fabrication process of the 3-D inductive coil, which can further advance the coil miniaturization. Experimental results show the microcoil with a 1.5 A, 1 kHz square-wave current input can induce a voltages of ~220 µV on the conducting wire with an impedance of ~0.2 Ω @ 1 kHz, 1 mm separation.
