ABSTRACT
723 blood sera from 537 patients of Regional Infectious Clinical Hospital, Astrakhan were obtained during high activity period of Rhipicephalus ticks (May-September 2015) and retrospectively studied for IgG/IgM to antigen of spotted fever group (SFG) Rickettsia. IgG and/or IgM to Rickettsia conorii were detected in 145 sera from 130 patients, and antibodies to R. sibirica (group-specific) were detected in 143 sera from 145. Antibodies to R. conorii were detected for 71,4% patients with Astrakhan spotted fever (ASF), for 28,4% patients with acute respiratory viral infection, for 19,1% patients with infection of unspecified etiology and for 40% patients having symptoms of a adenovirus infection. Acute rickettsiosis, provably ASF, is serologically validated for 71 patients. Dynamic of IgM/IgG to R. conorii in sera of patients having different preliminary diagnoses is discussed. IgM to R. conorii in sera of patients having adenovirus infection symptoms were detected at a later time as compared with others. For regions of high risk of R. conorii subsp. caspia infection the differentiation of diagnostic and anamnestic specific antibodies is very important. The absence of serological and molecular biological markers in third of patients with ASF symptoms is necessary to study. Preparations and algorithms for diagnosis of SFG rickettsioses are needed to improve.
Subject(s)
Rickettsia conorii , Spotted Fever Group Rickettsiosis/diagnosis , Animals , Boutonneuse Fever/diagnosis , Humans , Retrospective Studies , Rhipicephalus/microbiologyABSTRACT
The detection of antibodies to Core-antigen of virus of hepatitis C in test-systems for solid-phase immune-enzyme analysis with low optical density can be a result not only of true availability of antibodies but an effect of nonspecific reaction of blood serum. The diagnostic possibilities of immunochips to be used in immune-enzyme analysis for verification of availability of markers of viral hepatitis C were investigated in conditions of low positive reaction of blood serum to core-antigen. It is established that immunochips and immunoblots have similar specificity concerning detection of antibodies to Core-antigen. At that, in immunochips antibodies to nonstructural antigens of virus of hepatitis C were additionally detected in more than 90% of samples.
Subject(s)
Hepacivirus/isolation & purification , Hepatitis C Antibodies , Hepatitis C/diagnosis , Viral Core Proteins/isolation & purification , Adolescent , Adult , Aged , Female , Hepacivirus/pathogenicity , Hepatitis C/blood , Hepatitis C/virology , Hepatitis C Antibodies/blood , Hepatitis C Antibodies/immunology , Humans , Immunoglobulin G/blood , Immunoglobulin M/blood , Male , Middle Aged , Pregnancy , Viral Core Proteins/bloodABSTRACT
The new kit of reagents in format of the immunochip "ImmunoChip Borreliosis" for multiplex serologic analysis of mite-borne borreliosis demonstrated high diagnostic sensitivity and specificity. The percentage of detection of specific immunoglobulins was higher in "ImmunoChip Borreliosis" as compared with screening results in immune enzyme analysis. The high correlation between results of testing in immunochip and data of immune blotting is demonstrated to.
Subject(s)
Borrelia burgdorferi/isolation & purification , Ixodes/microbiology , Lyme Disease/diagnosis , Lyme Disease/microbiology , Animals , Antibodies, Bacterial/blood , Antibodies, Bacterial/immunology , Antibodies, Bacterial/isolation & purification , Borrelia burgdorferi/pathogenicity , Humans , Immunoglobulin G/blood , Immunoglobulin G/isolation & purification , Immunoglobulin M/blood , Immunoglobulin M/isolation & purification , Lyme Disease/blood , Lyme Disease/immunology , Sensitivity and Specificity , Serologic Tests/methodsABSTRACT
A test kit as an immunochip designed for the diagnosis of hepatic C virus (HCV) has a high sensitivity and specificity. Recombinant HCV antigens were separately immobilized on the activated slides together with internal controls. Serum test results were red by ScanArray Express. K-factor and corresponding value of cut-off were calculated for each antigen and internal controls. Comparative evaluation of the sensitivity and specificity of the immunochip was carried out by commercial ELISA test kits and linear blotting analyses on 448 blood samples containing and free from NCV antibodies.
Subject(s)
Hepatitis C Antibodies/blood , Hepatitis C/diagnosis , Humans , Immunoassay/instrumentation , Immunoassay/methods , Microarray Analysis , Sensitivity and Specificity , Serologic Tests/methodsABSTRACT
A preclinical trial of the vaccine HIVREPOL provided a complex of methods for assessing the identity and specific activity of vaccines against HIVIAIDS. The identity of "HIVREPOL" has been assessed by indirect enzyme immunoassay (EIA): the vaccine specifically binds the antibodies of the sera from HIV-infected individuals. Immune blot assay was the most informative method for assessing the identity of the candidate vaccine. The sera from HIVREPOL-vaccinated mice recognized the proteins gp41, p24, p55 of cultured HIV1 on "New-Lay-Blot1" strips. The bands corresponding to p24 were revealed in the line blots "Blot-HIV-1/2+O" and "INNO-LIA-HIV-Confirmation". The specific activity of the HIVREPOL vaccine was confirmed from the reactivity of sera of the mice vaccinated with recombinant proteins of the immunosorbents available in EIA test systems for the detection of HIV antibodies. Competitive EIA established the antigen-binding activity of sera from HIVREPOL-vaccinated mice against the native reference HIV-1 antigen.
