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1.
Med Sci Monit ; 24: 6129-6136, 2018 Sep 03.
Article in English | MEDLINE | ID: mdl-30175821

ABSTRACT

BACKGROUND WD40-encoding RNA antisense to p53 (Wrap53) has been implicated in cancer development. However, the role of Wrap53 remains unknown in colorectal cancer. The aim of this study was to elucidate the function of Wrap53 in colorectal cancer tumorigenesis and development. MATERIAL AND METHODS This study analyzed Wrap53 expression in colorectal cancer tissue specimens using The Cancer Genome Atlas data and tumor cell lines and assessed the effects of Wrap53 knockdown on regulation of cancer cell malignant phenotypes in vitro and in nude mouse xenografts. RESULTS Wrap53 expression was upregulated in colorectal cancer tissue specimens and cell lines. Knockdown of Wrap53 expression induced colorectal cancer cell line apoptosis and cell cycle arrest in the G1 phase, but reduced tumor cell line proliferation and invasion in vitro. Knockdown of Wrap53 in a nude mouse xenograft assay inhibited tumor cell line xenograft formation and growth. CONCLUSIONS Wrap53 is likely a potential oncogene or possesses oncogenic activity in colorectal cancer, promoting colorectal tumorigenesis. Targeting Wrap53 expression may represent a novel strategy for the control of colorectal cancer.


Subject(s)
Colorectal Neoplasms/genetics , Colorectal Neoplasms/metabolism , Telomerase/genetics , Telomerase/metabolism , Animals , Apoptosis/genetics , Carcinogenesis/genetics , Cell Line, Tumor , Cell Proliferation/genetics , Colorectal Neoplasms/pathology , Gene Knockdown Techniques , Heterografts , Humans , Mice , Mice, Nude , Molecular Chaperones , Neoplasm Invasiveness
2.
Med Sci Monit ; 23: 5330-5336, 2017 Nov 09.
Article in English | MEDLINE | ID: mdl-29118314

ABSTRACT

BACKGROUND Presently, studies of factors associated with drug-resistant tuberculosis (TB) focus on patients' socio-demographic characteristics and living habits, to the exclusion of biochemical indicators, especially immune factors. This study was carried out to determine whether immune factors are associated with drug-resistant TB. MATERIAL AND METHODS A total of 227 drug-resistant pulmonary TB patients and 225 drug-susceptible pulmonary TB patients were enrolled in this study. Information on socio-demographic characteristics and biochemical indicators were obtained through their clinical records. Non-conditional logistic regression was used to analyze the association of these indicators with drug-resistant TB. RESULTS There were significant differences in re-treatment, marital status, alanine aminotransferase (ALT), blood uric acid (BUA), carcino-embryonic antigen (CEA), T-spot, and CD3 and CD4 counts between the 2 groups. In multivariable analysis, re-treatment [Odds Ratio (OR)=5.290, 95% Confidence Interval [CI]=2.652-10.551); CD3 (OR=1.034, 95% CI=1.001-1.068); CD4 (OR=1.035, 95% CI =1.001-1.070) and IgM (OR=1.845, 95% CI=1.153-2.952) were associated with drug-resistant TB. CONCLUSIONS These results suggest the need for greater attention to re-treatment cases and immune function when treating drug-resistant TB.


Subject(s)
Immunologic Factors/metabolism , Tuberculosis, Multidrug-Resistant/immunology , Adult , Aged , Antitubercular Agents/therapeutic use , Biomarkers, Pharmacological/blood , CD3 Complex/blood , CD3 Complex/immunology , CD4 Antigens/blood , CD4 Antigens/immunology , Case-Control Studies , China , Female , Humans , Immunity, Cellular/immunology , Immunoglobulin M/blood , Immunoglobulin M/immunology , Immunologic Factors/blood , Immunologic Factors/physiology , Logistic Models , Male , Middle Aged , Odds Ratio , Risk Factors , Tuberculosis, Multidrug-Resistant/metabolism
3.
Iran J Public Health ; 44(2): 263-8, 2015 Feb.
Article in English | MEDLINE | ID: mdl-25905061

ABSTRACT

BACKGROUND: The MIRU-VNTR polymorphism and katG463 mutation are used to genotype the mycobacterium tuberculosis, but the correlation between them and INH-resistance were unknown. This study was aimed to explore whether ETRE polymorphism and katG463 mutation could predict the INH-resistance, and the relationship between ETRE polymorphism and katG463 mutation. METHODS: The ETRE, katG463 mutation and drug resistance information of 109 M. tuberculosis strains were collected from online public database. We constructed the predictive diagnostic tool of ETRE polymorphism and katG463 mutation. Chi-square test was used to analyze the relationship between ETRE polymorphism, katG463 mutation and INH-resistance. ROC curve analysis and Z-test were used to evaluate the predictive ability of ETRE and katG463. The relationship between ETRE polymorphism and katG463 mutation was analyzed with Spearman correlation analysis. RESULTS: The mutation rate of katG463 was 27.50%, and the h value of ETRE polymorphism was 0.67. KatG463 mutation was associated with INH resistance (OR=3.72). The INH drug resistance rate in VNTR≧5 group was 3.43 times higher than that in VNTR≦3 group (χ(2) =24.77, P<0.01), and there was no significant difference of INH resistance between the VNTR=4 group and VNTR≦3 group. The areas under the ROC curve of two loci prediction diagnostic tools were 0.64 and 0.70 respectively. The katG463 mutation was significantly related to the ETRE polymorphism (r=0.79, P<0.01). CONCLUSION: Both katG463 mutation and the ETRE polymorphism can predict the INH-resistance of tuberculosis. The katG463 mutation was associated with ETRE VNTR polymorphism.

4.
Article in English | MEDLINE | ID: mdl-26867388

ABSTRACT

Men who have sex with men (MSM) are at risk for contracting hu- man immunodeficiency virus (HIV) infection. The objective of this study was to explore the characteristics and factors influencing unprotected anal intercourse (UAI) among MSM in Fuyang, China in order to develop an intervention program to prevent the spread of HIV infection among MSM. We conducted this cross sectional study among 413 MSM in 2013. Participants completed an interviewer- administered questionnaire and were tested for HIV and syphilis infections. Three hundred fifty of 413 subjects reported sexual activity with a male partner during the previous 6 months; of these 27(7.7%) had unprotected sex. Forty-four subjects had sex with a female partner during the previous 6 months; of these 25 (58.1%) had unprotected sex. The frequency of having unprotected sex with a female was significantly greater than with a male (χ2 = 84.52, p < 0.001). Multivariate logistic analysis showed education level (OR = 0.45, p = 0.003), length of time of current residence (OR = 0.47, p = 0.014), knowledge about HIV infection (OR = 0.09, p = 0.022) and integrated interventions (OR = 0.32, p < 0.001) were all significantly associated with UAI. High-risk sex behavior was common among the study population. A targeted interventions needs to be developed urgently.


Subject(s)
Bisexuality , Condoms/statistics & numerical data , Health Knowledge, Attitudes, Practice , Homosexuality, Male , Sexual Behavior/statistics & numerical data , Unsafe Sex/statistics & numerical data , Adult , China/epidemiology , Cross-Sectional Studies , Educational Status , HIV Infections/epidemiology , Humans , Logistic Models , Male , Multivariate Analysis , Risk Factors , Sexual Partners , Young Adult
5.
Zhonghua Liu Xing Bing Xue Za Zhi ; 34(5): 515-9, 2013 May.
Article in Chinese | MEDLINE | ID: mdl-24016446

ABSTRACT

OBJECTIVE: To analyze the prevalence and differences between gender and grades of suicide ideation among middle school students in China from 2000 to 2012 so as to provide basis for suicide prevention among middle school students. METHODS: Electronic search strategy was carried out, using PubMed, Wanfang Database, VIP Database, China National Knowledge Infrastructure (CNKI) and CBM to collect data on suicide ideation among middle school students. Fixed effects model or random effects model was employed according to statistical tests for the homogeneity. Publication bias was assessed by rank correlation test. RESULTS: 40 papers were included for meta-analysis, with a total sample size of 320 375. The combined prevalence of suicide ideation was 17.99% (95%CI: 16.59% - 19.49%). Prevalence rates of suicide ideation were stratified by factors as gender and grade at school. Pooled prevalence rates on suicide ideation were as follows: 14.71% (95%CI: 13.42% - 16.11%) and 19.92% (95%CI: 19.30% - 21.64%), P < 0.05 for boys and girls;16.94% (95%CI: 15.35% - 18.66%) and 19.01% (95%CI: 17.23% - 20.93%), P < 0.05 for senior or junior high school students, respectively. CONCLUSION: There were differences in the prevalence of suicide ideation among middle school students between genders and grades in China.


Subject(s)
Suicide/statistics & numerical data , Adolescent , China/epidemiology , Female , Humans , Male , Prevalence , Risk Factors , Schools , Students , Surveys and Questionnaires
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