ABSTRACT
To detect and analyze the changes of microorganisms in expressed prostatic secretion (EPS) of patients with IIIB prostatitis before and after low-intensity pulsed ultrasound (LIPUS) treatment, and to explore the mechanism of LIPUS in the treatment of chronic prostatitis (CP). 25 patients (study power was estimated using a Dirichlet-multinomial approach and reached 96.5% at α = 0.05 using a sample size of 25) with IIIB prostatitis who were effective in LIPUS treatment were divided into two groups before and after LIPUS treatment. High throughput second-generation sequencing technique was used to detect and analyze the relative abundance of bacterial 16 s ribosomal variable regions in EPS before and after treatment. The data were analyzed by bioinformatics software and database, and differences with P < 0.05 were considered statistically significant. Beta diversity analysis showed that there was a significant difference between groups (P = 0.046). LEfSe detected four kinds of characteristic microorganisms in the EPS of patients with IIIB prostatitis before and after LIPUS treatment. After multiple comparisons among groups by DESeq2 method, six different microorganisms were found. LIPUS may improve patients' clinical symptoms by changing the flora structure of EPS, stabilizing and affecting resident bacteria or opportunistic pathogens.
Subject(s)
Prostate , Prostatitis , Ultrasonic Waves , Humans , Male , Prostatitis/therapy , Prostatitis/microbiology , Prostatitis/metabolism , Prostate/microbiology , Prostate/metabolism , Prostate/pathology , Adult , Bacteria/metabolism , Bacteria/genetics , Middle Aged , Ultrasonic Therapy/methods , Microbiota , RNA, Ribosomal, 16S/geneticsABSTRACT
Rhizoma Corydalis is an important Chinese medicinal herb. In this paper, we employed ISSR data to explore the genetic variation in domesticated populations and wild populations of the species. The average of within-population ISSR diversity in cultivated populations (PPF=25.32%, Hpop=0.094) was lower than that in wild populations (PPF=47.70%, Hpop=0.144). Cultivated populations (PhiST=0.515, GST=0.429) have a greater proportion of their genetic variability distributed among populations than wild populations (PhiST=0.277, GST=0.226). Based on hierarchical estimates of variance components, significant statistical differences (57.77%, P<0.001) were found between the wild and cultivated groups. The low levels of genetic diversity within cultivated populations and high levels of genetic differentiation among populations/groups may result from artificial selection, the mode of clonal propagation, and only limited exchange of material among localities. Finally, some suggestions for conservation and efficient management of the genetic resources of this important medicinal herb are proposed.
Subject(s)
Corydalis/genetics , Minisatellite Repeats , Plants, Medicinal/genetics , Polymorphism, Genetic , Genetic Markers , PhylogenyABSTRACT
OBJECTIVE: To establish the AFLP Fingerprinting system in the germplasm of Atractylodes macrocephala Koidz. METHODS: 10 wild or cultivated Atractylodes macrocephala were used for AFLP fingerprinting analysis by EcoRI and MseI restriction enzymes with silver staining. RESULTS: Using 2X CTAB buffer extraction method can obtain the best genomic DNA samples. According to the AFLP polymorphism, sixteen out of forty primer pairs were selected to be suitable for AFLP analysis. Total 3003 polymorphic bands were obtained from the 16 sets of primer. Based on the AFLP results, the 10 samples of Atractylodes macrocephala germplasm were classified into four types. CONCLUSION: The establishment of the AFLP fingerprinting system in Atractylodes macrocephala will be used in the identification of germplasms and breeding of the species.
Subject(s)
Amplified Fragment Length Polymorphism Analysis , Atractylodes/genetics , Genetic Markers , Plants, Medicinal/genetics , Atractylodes/classification , Cluster Analysis , DNA Fingerprinting/methods , DNA, Plant/analysis , DNA, Plant/isolation & purification , Genome, Plant/genetics , Phylogeny , Polymorphism, GeneticABSTRACT
OBJECTIVE: To investigate the genetic diversity of main germplasm of Atractylodes macrocephala in China and the genetic differentiation of the germplasm of A. macrocephala. METHOD: A molecular marker ISSR was used to analyze the genetic diversity of 7 populations of A. macrocephala and a population of A. lancea. RESULT: Twelve primers were used in the PCR amplification of 86 samples of A. macrocephala and 5 samples of A. lancea. Sixty-three bands with sizes ranged from 100 to 2500 bp were generated from 12 primers. Of all the 63 bands, 55 bands were polymorphic among 86 individuals of A. macrocephala, the percentage of polymorphic bands were 87.30% at the species level. The percentage of polymorphic bands (PPL) for a single population ranged from 58.73% to 71.43% (mean, 64.85%). Among the 7 populations, a population from Panan, GM exhibited highest variability (PPL =71.43%; HE = 0.2835; I = 0.4267). A dendrogram constructed by an unweighted pair group method of cluster analysis showed that populations from Panan constructed one branch and separated from other populations. In the AMOVA analysis, low level of genetic differentiation among populations was detected, 90.52% of the variability existed in population. CONCLUSION: The genetic diversity of cultivated A. macrocephala in China is high, which is good for the production of high quality herb medicine.
