The unseen invaders: Tracking phylogeographic dynamics and genetic diversity of cryptic Pomacea canaliculata and P. maculata (Golden apple snails) across Taiwan.

The cryptic invasion of golden apple snails (Pomacea canaliculata and P. maculata) in Taiwan has caused significant ecological and economical damage over the last few decades, however, their management remains difficult due to inadequate taxonomic identification, complex phylogeny, and limited population genetic information. We aim to understand the current distribution, putative population of origin, genetic diversity, and potential path of cryptic invasion of Pomacea canaliculata and P. maculata across Taiwan to aid in improved mitigation approaches. The present investigation conducted a nationwide survey with 254 samples collected from 41 locations in 14 counties or cities across Taiwan. We identified P. canaliculata and P. maculata based on mitochondrial COI and compared their genetic diversity across Taiwan, as well as other introduced and native countries (based on publicly available COI data) to understand the possible paths of invasion to Taiwan. Based on mitochondrial COI barcoding, sympatric and heterogeneous distributions of invasive P. canaliculata and P. maculata were noted. Our haplotype analysis and mismatch distribution results suggested multiple introductions of P. canaliculata in Taiwan was likely originated directly from Argentina, whereas P. maculata was probably introduced from a single, or a few, introduction event(s) from Argentina and Brazil. Our population genetic data further demonstrated a higher haplotype and genetic diversity for P. canaliculata and P. maculata in Taiwan compared to other introduced regions. Based on our current understanding, the establishment of P. canaliculata and P. maculata is alarming and widespread beyond geopolitical borders, requiring a concerted and expedited national and international invasive species mitigation program.

Transformative and sustainable insights of agricultural waste-based adsorbents for water defluoridation: Biosorption dynamics, economic viability, and spent adsorbent management.

With the progression of civilization, the harmony within nature has been disrupted, giving rise to various ecocidal activities that are evident in every spheres of the earth. These activities have had a profound and far-reaching impact on global health. One significant example of this is the presence of fluoride in groundwater exceeding acceptable limits, resulting in the widespread occurrence of "Fluorosis" worldwide. It is imperative to mitigate the concentration of fluoride in drinking water to meet safety standards. While various defluoridation techniques exist, they often have drawbacks. Biosorption, being a simple, affordable and eco-friendly method, has gained preference for defluoridation. However, its limited commercialization underscores the pressing need for further research in this domain. This comprehensive review article offers a thorough examination of the defluoridation potential of agro-based adsorbents, encompassing their specific chemical compositions and preparation methods. The review presents an in-depth discussion of the factors influencing fluoride biosorption and conducts a detailed exploration of adsorption isotherm and adsorption kinetic models to gain a comprehensive understanding of the nature of the adsorption process. Furthermore, it evaluates the commercial viability through an assessment of regeneration potential and a cost analysis of these agro-adsorbents, with the aim of facilitating the scalability of the defluoridation process. The elucidation of the adsorption mechanism and recommendations for overcoming challenges in large-scale implementation offer a comprehensive outlook on this eco-friendly and sustainable approach to fluoride removal. In summary, this review article equips readers with a lucid understanding of agro-adsorbents, elucidates their ideal conditions for improved performance, offers a more profound insight into the fluoride biosorption mechanism, and introduces the concept of effective spent adsorbent management.

Microbial induced carbonate precipitation for remediation of heavy metals, ions and radioactive elements: A comprehensive exploration of prospective applications in water and soil treatment.

Improper disposal practices have caused environmental disruptions, possessing by heavy metal ions and radioactive elements in water and soil, where the innovative and sustainable remediation strategies are significantly imperative in last few decades. Microbially induced carbonate precipitation (MICP) has emerged as a pioneering technology for remediating contaminated soil and water. Generally, MICP employs urease-producing microorganisms to decompose urea (NH2CONH2) into ammonium (NH4+and carbon dioxide (CO2), thereby increasing pH levels and inducing carbonate precipitation (CO32-), and effectively removing remove contaminants. Nonetheless, the intricate mechanism underlying heavy metal mineralization poses a significant challenge, constraining its application in contaminants engineering, particularly in the context of prolonged heavy metal leaching over time and its efficacy in adverse environmental conditions. This review provides a comprehensive idea of recent development of MICP and its application in environmental engineering, examining metabolic pathways, mineral precipitation mechanisms, and environmental factors as well as providing future perspectives for commercial utilization. The use of ureolytic bacteria in MICP demonstrates cost-efficiency, environmental compatibility, and successful pollutant abatement over tradition bioremediation techniques, and bio-synthesis of nanoparticles. limitations such as large-scale application, elevated Ca2+levels in groundwater, and gradual contaminant release need to be overcome. The possible future research directions for MICP technology, emphasizing its potential in conventional remediation, CO2 sequestration, bio-material synthesis, and its role in reducing environmental impact for long-term economic benefits.

Insight interactions of engineered nanoparticles with aquatic higher plants for phytoaccumulation, phytotoxicity, and phytoremediation applications: A review.

With the growing age of human civilization, industrialization has paced up equally which is followed by the innovation of newer concepts of science and technology. One such example is the invention of engineered nanoparticles and their flagrant use in widespread applications. While ENPs serve their intended purposes, they also disrupt the ecological balance by contaminating pristine aquatic ecosystems. This review encompasses a comprehensive discussion about the potent toxicity of ENPs on aquatic ecosystems, with a particular focus on their impact on aquatic higher plants. The discussion extends to elucidating the fate of ENPs upon release into aquatic environments, covering aspects ranging from morphological and physiological effects to molecular-level phytotoxicity. Furthermore, this level of toxicity has been correlated with the determination of competent plants for the phytoremediation process towards the mitigation of this ecological stress. However, this review further illustrates the path of future research which is yet to be explored. Determination of the genotoxicity level of aquatic higher plants could explain the entire process comprehensively. Moreover, to make it suitable to be used in natural ecosystems phytoremediation potential of co-existing plant species along with the presence of different ENPs need to be evaluated. This literature will undoubtedly offer readers a comprehensive understanding of the stress induced by the irresponsible release of engineered nanoparticles (ENP) into aquatic environments, along with insights into the resilience characteristics of these pristine ecosystems.

The role of bacterial exopolysaccharides (EPS) in the synthesis of antimicrobial silver nanomaterials: A state-of-the-art review.

The emergence of multi-drug resistant (MDR) pathogens poses a significant global health concern due to the failure of conventional medical treatment. As a result, the development of several metallic (Ag, Au, Zn, Ti, etc.) nanoparticles, has gained prominence as an alternative to conventional antimicrobial therapies. Among these, green-synthesized silver nanoparticles (AgNPs) have gained significant attention due to their notable efficiency and broad spectrum of antimicrobial activity. Bacterial exopolysaccharides (EPS) have recently emerged as a promising biological substrate for the green synthesis of AgNPs. EPS possess polyanionic functional groups (hydroxyl, carboxylic, sulfate, and phosphate) that effectively reduce and stabilize AgNPs. EPS-mediated AgNPs exhibit a wide range of antimicrobial activity against various pathogenic microbes, including Gram-positive and Gram-negative bacteria, as well as fungi. The extraction and purification of bacterial EPS play a vital role in obtaining high-quality and -quantity EPS for industrial applications. This study focuses on the comprehensive methodology of EPS extraction and purification, encompassing screening, fermentation optimization, pretreatment, protein elimination, precipitation, and purification. The review specifically highlights the utilization of bacterial EPS-mediated AgNPs, covering EPS extraction, the synthesis mechanism of green EPS-mediated AgNPs, their characterization, and their potential applications as antimicrobial agents against pathogens. These EPS-mediated AgNPs offer numerous advantages, including biocompatibility, biodegradability, non-toxicity, and eco-friendliness, making them a promising alternative to traditional antimicrobials and opening new avenues in nanotechnology-based approaches to combat microbial infections.

Biologically-induced synthetic manganese carbonate precipitate (BISMCP) for potential applications in heavy metal removal.

Heavy metal pollution of water is a burning issue of today's world. Among several strategies involved for heavy metal remediation purpose, biomineralization has shown great potential. Of late, research has been focused on developing effective mineral adsorbents with reduced time and cost consumption. In this present paper, the Biologically-Induced Synthetic Manganese Carbonate Precipitate (BISMCP) was produced based on the biologically-induced mineralization method, employing Sporosarcina pasteurii in aqueous solutions containing urea and MnCl2. The prepared adsorbent was characterized using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), SEM-energy dispersive X-ray spectroscopy (SEM-EDX), X-ray diffraction (XRD) and BET surface area analyzer. EDX analysis showed the elements in the crystal BISMCP were Mn, C, and O. XRD result of BISMCP determined the crystal structure, which is close to rhodochrosite (MnCO3). Spectral peaks of FTIR at 1641.79 cm-1 confirmed the appearance of C[bond, double bond]O binding, with strong stretching of CO32- in Amide I. From the six kinds of BISMCP produced, sample MCP-6 has the higher specific surface area by BET analysis at 109.01 m2/g, with pore size at 8.76 nm and higher pore volume at 0.178 cm3/g. These specifications will be suitable as an adsorbent for heavy metal removal by adsorption process. This study presents a preliminary analysis of the possibility of BISMCP for heavy metals adsorption using ICP multi-element standard solution XIII (As, Cr, Cd, Cu, Ni, and Zn). BISMCP formed from 0.1 MnCl2 and 30 ml of bacteria volume (MCP-6) produced a better adsorbent material than others concentrations, with the adsorption efficiency of total As at 98.9%, Cr at 97.0%, Cu at 94.7%, Cd at 88.3%, Zn at 48.6%, and Ni at 29.5%. Future work could be examined its efficiency adsorbing individual heavy metals.

A mechanistic insight into the shrinkage and swelling of Ca-montmorillonite upon adsorption of chain-like ranitidine in an aqueous system.

Adsorption behavior of ranitidine hydrochloride (RT) on a Ca-montmorillonite (SAz-1) was studied in aqueous system through batch experiments. The adsorption kinetics revealed that the equilibrium reached within 0.25 h and the data fitted well to the pseudo-second order kinetic equation (R2 = 0.98). The maximum RT adsorption capacity of SAz-1 was 369.2 mg/g and the adsorption isotherm data followed the Langmuir model (R2 = 0.99). The adsorption of RT and desorption of exchangeable cations from the clay mineral were linearly correlated, suggesting that cation exchange was the dominant mechanism of RT adsorption. The XRD examination of RT-adsorbed SAz-1 samples (unsaturated/saturated) after heating enabled the calculation of RT occupied area in the interlayer of the clay mineral. The results suggested that adsorbed-RT at low loading rate could lay on the internal surfaces in a free style to reduce the basal spacing (d001 value) of SAz-1. When the RT loading rate was increased, a limited surface space enforced more RT molecules to lay in a tilted style and caused interlayer swelling of SAz-1 increasing the d001 value. The trend of rising decomposition temperature of RT with increasing RT loading rates confirmed intercalation of RT molecules in SAz-1. Infrared spectral analysis revealed the participation of amide and furan groups of RT in binding between RT and SAz-1. Thus, this study indicated that SAz-1 is an efficient adsorbent to remove RT from contaminated water, and the chain-like molecular structure of RT could cause an irregular change in the basal spacing of swelling type clay minerals.

New aspects of lipopeptide-incorporated nanoparticle synthesis and recent advancements in biomedical and environmental sciences: a review.

The toxicity of metal nanoparticles has introduced promising research in the current scenario since an enormous number of people have been potentially facing this problem in the world. The extensive attention on green nanoparticle synthesis has been focussed on as a vital step in bio-nanotechnology to improve biocompatibility, biodegradability, eco-friendliness, and huge potential utilization in various environmental and clinical assessments. Inherent influence on the study of green nanoparticles plays a key role to synthesize the controlled and surface-influenced molecule by altering the physical, chemical, and biological assets with the provision of various precursors, templating/co-templating agents, and supporting solvents. However, in this article, the dominant characteristics of several kinds of lipopeptide biosurfactants are discussed to execute a critical study of factors affecting synthesis procedure and applications. The recent approaches of metal, metal oxide, and composite nanomaterial synthesis have been deliberated as well as the elucidation of the reaction mechanism. Furthermore, this approach shows remarkable boosts in the production of nanoparticles with the very less employed harsh and hazardous processes as compared to chemical or physical method-based nanoparticle synthesis. This study also shows that the advances in strain selection for green nanoparticle production could be a worthwhile and strong economical approach in futuristic medical science research.

Environmental DNA analysis as an emerging non-destructive method for plant biodiversity monitoring: a review.

Environmental DNA (eDNA) analysis has recently transformed and modernized biodiversity monitoring. The accurate detection, and to some extent quantification, of organisms (individuals/populations/communities) in environmental samples is galvanizing eDNA as a successful cost and time-efficient biomonitoring technique. Currently, eDNA's application to plants remains more limited in implementation and scope compared to animals and microorganisms. This review evaluates the development of eDNA-based methods for (vascular) plants, comparing its performance and power of detection with that of traditional methods, to critically evaluate and advise best-practices needed to innovate plant biomonitoring. Recent advancements, standardization and field applications of eDNA-based methods have provided enough scope to utilize it in conservation biology for numerous organisms. Despite our review demonstrating only 13% of all eDNA studies focus on plant taxa to date, eDNA has considerable environmental DNA has considerable potential for plants, where successful detection of invasive, endangered and rare species, and community-level interpretations have provided proof-of-concept. Monitoring methods using eDNA were found to be equal or more effective than traditional methods; however, species detection increased when both methods were coupled. Additionally, eDNA methods were found to be effective in studying species interactions, community dynamics and even effects of anthropogenic pressure. Currently, elimination of potential obstacles (e.g. lack of relevant DNA reference libraries for plants) and the development of user-friendly protocols would greatly contribute to comprehensive eDNA-based plant monitoring programs. This is particularly needed in the data-depauperate tropics and for some plant groups (e.g., Bryophytes and Pteridophytes). We further advocate to coupling traditional methods with eDNA approaches, as the former is often cheaper and methodologically more straightforward, while the latter offers non-destructive approaches with increased discrimination ability. Furthermore, to make a global platform for eDNA, governmental and academic-industrial collaborations are essential to make eDNA surveys a broadly adopted and implemented, rapid, cost-effective and non-invasive plant monitoring approach.

Heavy metals distribution and ecological risk assessment including arsenic resistant PGPR in tidal mangrove ecosystem.

Heavy metals (HM) are the major proximate drivers of pollution in the mangrove ecosystem. Therefore, ecological risk (ER) due to HM distribution/concentration in core-sediment of Puzi mangrove region (Taiwan) was examined with tidal influence (TI) along with indigenous rhizospheric bacteria (IRB). The HM concentration was observed higher at active-tidal-sediment compared to partially-active-sediment. Geo-accumulation index (Igeo) and contamination factor (CF) indicated the tidal-sediment was highly contaminated with arsenic (As) and moderately contaminated with Lead (Pb) and Zinc (Zn). However, the pollution loading index (PLI) and degree of contamination (Cd) exhibited 'no pollution' and 'low-moderate degree of contamination', in the studied region respectively. The isolated IRB (Priestia megaterium, Bacillus safenis, Bacillus aerius, Bacillus subtilis, Bacillus velenzenesis, Bacillus lichenoformis, Kocuria palustris, Enterobacter hormaechei, Pseudomonus fulva, and Paenibacillus favisporus; accession number OM979069-OM979078) exhibited the arsenic resistant behavior with plant-growth-promoting characters (IAA, NH3, and P-solubilization), which can be used in mangrove reforestation and bioremediation of HM.

Characteristics of Doped TiO2 Nanoparticle Photocatalysts Prepared by the Rotten Egg White.

In this study, expired egg white was used as a template, and a sol-gel method was employed to prepare pure-phase TiO2 nano-powder and mixed-phase powders doped with NaF and NaI. The influences of different calcination temperatures, doping elements, and doping amounts during the preparation process on the photocatalytic performance and activity of the prepared TiO2 powders were studied. The results of the experiments showed that the F-doped TiO2 had the highest photocatalytic activity when the doping amount was 1.2%, as examined by EDS, where the sintering temperature was 500 °C. F-doped TiO2 nanoparticles were also synthesized by the sol-gel method using tetrabutyl titanate and NaF mixed with expired egg white protein as the precursor. The F-TiO2 photocatalyst was characterized using FE-SEM, HR-TEM, EDS, XPS, and UV-Vis, and the photocatalytic activity was evaluated by photodegradation of methylene blue under visible light. The results showed that doping with F reduced the energy band gap (3.04 eV) of TiO2, thereby increasing the photocatalytic activity in the visible-light region. The visible-light wavelength range and photocatalytic activity of the catalyst were also affected by the doping amount.

Novel MOF-808 metal-organic framework as highly efficient adsorbent of perfluorooctane sulfonate in water.

Perfluorooctane sulfonate (PFOS) is a highly persistent contaminant of emerging concern causing harmful effects to human and ecosystem health. In this study, a novel MOF-808 metal-organic framework (MOF) was prepared and evaluated for adsorptive removal of PFOS from aqueous solution. The MOF-808 had high specific surface area (SSA; 1610 m2/g) and was structurally stable in aqueous medium for 7 days under different pH conditions. The MOF-808 reached PFOS adsorption equilibrium within 30 min (at 500 mg/L initial PFOS) and attained the maximum adsorption capacity of 939 mg/g at pH 4.1 - 5.4 (with 50 - 500 mg/L initial PFOS). The PFOS adsorption capacity of MOF-808 was unaffected at pH 2 to 7, but gradually decreased at pH > 7. High SSA, favorable pore size and abundant active adsorption sites on MOF-808 triggered high PFOS adsorption onto the adsorbent. The PFOS adsorption process was endothermic and spontaneous in nature. Electrostatic interaction between the cationic central cluster ([Zr6O4(OH)4]12+) of MOF-808 and PFOS anion was identified as the key mechanism of PFOS adsorption onto MOF-808, as evident from the infrared spectroscopic investigation of the adsorbent. This study suggests that MOF-808 can be considered as a highly efficient adsorbent for PFOS removal from water and warrants future research to evaluate the application and performance of the material under wastewater conditions.

Reinforcement of Environmental DNA Based Methods (Sensu Stricto) in Biodiversity Monitoring and Conservation: A Review.

Recently developed non-invasive environmental DNA-based (eDNA) techniques have enlightened modern conservation biology, propelling the monitoring/management of natural populations to a more effective and efficient approach, compared to traditional surveys. However, due to rapid-expansion of eDNA, confusion in terminology and collection/analytical pipelines can potentially jeopardize research progression, methodological standardization, and practitioner adoption in several ways. Present investigation reflects the developmental progress of eDNA (sensu stricto) including highlighting the successful case studies in conservation management. The eDNA technique is successfully relevant in several areas of conservation research (invasive/conserve species detection) with a high accuracy and authentication, which gradually upgrading modern conservation approaches. The eDNA technique related bioinformatics (e.g., taxon-specific-primers MiFish, MiBird, etc.), sample-dependent methodology, and advancement of sequencing technology (e.g., oxford-nanopore-sequencing) are helping in research progress. The investigation shows that the eDNA technique is applicable largely in (i) early detection of invasive species, (ii) species detection for conservation, (iii) community level biodiversity monitoring, (iv) ecosystem health monitoring, (v) study on trophic interactions, etc. Thus, the eDNA technique with a high accuracy and authentication can be applicable alone or coupled with traditional surveys in conservation biology. However, a comprehensive eDNA-based monitoring program (ecosystem modeling and function) is essential on a global scale for future management decisions.

Variation of Microbial Diversity in Catastrophic Oil Spill Area in Marine Ecosystem and Hydrocarbon Degradation of UCMs (Unresolved Complex Mixtures) by Marine Indigenous Bacteria.

The study targeted an assessment of microbial diversity during oil spill in the marine ecosystem (Kaohsiung port, Taiwan) and screened dominant indigenous bacteria for oil degradation, as well as UCM weathering. DO was detected lower and TDS/conductivity was observed higher in oil-spilled area, compared to the control, where a significant correlation (R2 = 1; P < 0.0001) was noticed between DO and TDS. The relative abundance (RA) of microbial taxa and diversities (> 90% similarity by NGS) were found higher in the boundary region of spilled-oily-water (site B) compared to the control (site C) and center of the oil spill area (site A) (BRA/diversity > CRA/diversity > ARA/diversity). The isolated indigenous bacteria, such as Staphylococcus saprophyticus (CYCTW1), Staphylococcus saprophyticus (CYCTW2), and Bacillus megaterium (CYCTW3) degraded the C10-C30 including UCM of oil, where Bacillus sp. are exhibited more efficient, which are applicable for environmental cleanup of the oil spill area. Thus, the marine microbial diversity changes due to oil spill and the marine microbial community play an important role to biodegrade the oil, besides restoring the catastrophic disorders through changing their diversity by ecological selection and adaptation process.

A novel BMSN (biologically synthesized mesoporous silica nanoparticles) material: synthesis using a bacteria-mediated biosurfactant and characterization.

Mesoporous materials (MMs) have recently been applied as advanced nanomaterials in different fields (separation, catalysis, adsorption etc.). Synthesis of MMs by chemical surfactants is not ecofriendly. This study focused on the biological synthesis of a MM by sol-gel method, using a Bacillus subtilis BBK006-mediated surfactant (template) and a precursor (TEOS). The biologically synthesized mesoporous silica nanoparticles (BMSN) were formed at calcination temperatures of 450-600 °C. The BMSN comprise Si and O elements with specific weights of 56.09% and 42.13% respectively, where the atomic% was detected to be 41.79% and 55.10%, respectively. The phase identity of the synthesized particles (61-300 nm uniform spherical shape; surface area: 8.2616 m2 g-1; pore diameter at 550 °C: 14.8516 nm) was confirmed with wide-angle XRD (10°-81°). A typical type IV isotherm was exhibited (BET curves) following IUPAC nomenclature and confirmed the mesoporous nature. The green-synthesized biosurfactant-mediated BMSN is an environmentally promising material to apply in biomedical science (e.g., antimicrobial activity, drug delivery, CMC, anticancer activity) and oil spill management.

Advanced application of nano-technological and biological processes as well as mitigation options for arsenic removal.

Arsenic (As) removal is a huge challenge, since several million people are potentially exposed (>10 µg/L World Health Organization guideline limit) through As contaminated drinking water worldwide. Review attempts to address the present situation of As removal, considering key topics on nano-technological and biological process and current progress and future perspectives of possible mitigation options have been evaluated. Different physical, chemical and biological methods are available to remove As from contaminated water/soil/wastes, where removal efficiency mainly depends on absorbent type, initial adsorbate concentration, speciation and interfering species. Oxidation is an important pretreatment step in As removal, which is generally achieved by several media such as O2/O3, HClO, KMnO4 and H2O2. The Fe-based-nanomaterials (α/ß/γ-FeOOH, Fe2O3/Fe3O4-γ-Fe2O3), Fe-based-composite-compounds, activated-Al2O3, HFO, Fe-Al2O3, Fe2O3-impregnated-graphene-aerogel, iron-doped-TiO2, aerogel-based- CeTiO2, and iron-oxide-coated-manganese are effective to remove As from contaminated water. Biological processes (phytoremediation/microbiological) are effective and ecofriendly for As removal from water and/or soil environment. Microorganisms remove As from water, sediments and soil by metabolism, detoxification, oxidation-reduction, bio-adsorption, bio-precipitation, and volatilization processes. Ecofriendly As mitigation options can be achieved by utilizing an alternative As-safe-aquifer, surface-water or rainwater-harvesting. Application of hybrid (biological with chemical and physical process) and Best-Available-Technologies (BAT) can be the most effective As removal strategy to remediate As contaminated environments.

Reuse of Immobilized Weissella cibaria PN3 for Long-Term Production of Both Extracellular and Cell-Bound Glycolipid Biosurfactants.

Lactic acid bacteria (LABs) are generally recognized as safe (GRAS), and therefore, LAB biosurfactants are beneficial with negligible negative impacts. This study aims to maintain the biosurfactant producing activity of an LAB strain, Weissella cibaria PN3, by immobilizing the bacterial cells on a commercial porous carrier. For biosurfactant production, 2% soybean oil was used as the carbon source. After 72 h, immobilized cells were reused by replacing production medium. The extracellular and cell-bound biosurfactants were extracted from the resulting cell-free broth and cell pellets, respectively. SEM images of used immobilizing carriers showed increased surface roughness and clogged pores over time. Thus, the immobilizing carriers were washed in PBS buffer (pH 8.0) before reuse. To maintain biosurfactant production activity, immobilized cells were reactivated every three production cycles by incubating the washed immobilizing carriers in LB medium for 48 h. The maximum yields of purified extracellular (1.46 g/L) and cell-bound biosurfactants (1.99 g/L) were achieved in the 4th production cycle. The repeated biosurfactant production of nine cycles were completed within 1 month, while only 2 g of immobilized cells/L were applied. The extracellular and cell-bound biosurfactants had comparable surface tensions (31 - 33 mN/m); however, their CMC values were different (1.6 and 3.2 g/L, respectively). Both biosurfactants had moderate oil displacement efficiency with crude oil samples but formed emulsions well with gasoline, diesel, and lavender, lemongrass and coconut oils. The results suggested that the biosurfactants were relatively hydrophilic. In addition, the mixing of both biosurfactants showed a synergistic effect, as seen from the increased emulsifying activity with palm, soybean and crude oils. The biosurfactants at 10 - 16 mg/mL showed antimicrobial activity toward some bacteria and yeast but not filamentous fungi. The molecular structures of these biosurfactants were characterized by FTIR as different glycolipid congeners. The biosurfactant production process by immobilized Weissella cibaria PN3 cells was relatively cheap given that two types of biosurfactants were simultaneously produced and no new inoculum was required. The acquired glycolipid biosurfactants have high potential to be used separately or as mixed biosurfactants in various products, such as cleaning agents, food-grade emulsifiers and cosmetic products.

Efficient option of industrial wastewater resources in cement mortar application with river-sand by microbial induced calcium carbonate precipitation.

The industrial wastewater disposal has been growing attention for environmental protection and resource substitution, current decades. Similarly, the durability enhancement of concrete has increased attention by microbial induced CaCO3 precipitation (MICP) process (biocalcification). However, ecofriendly utilization of industrial wastewater in concrete formation is unstudied so far. The present study was carried out to evaluate the effect of industrial wastewater on the formation of cement mortar, compressive strength and water absorption. The biocement mortar strength (y) increased (y = 0.5295×2 + 1.6019×+251.05; R2 = 0.9825) with increasing percentage of organic wastewater (x) (BM0 - BM100) by MICP, where highest strength (280.75 kgf/cm2) was observed on BM100 (100% wastewater), compared to control (252.05 kgf/cm2). The water absorption (y) of biocement mortar decreases (y = -0.0251×2-0.103× + 15.965; R2 = 0.9594) with increment of wastewater (x) (%) (BM0 - BM100), where a minimum-water-absorption (14.42%) observed on BM100, compared to control (15.89%). SEM micrograph and XRD shows the formation of most-distinctive CaCO3 crystallization (aragonite/calcite) (acicular, brick shape, massive and stacked structure) inside biocement mortar (BM100), which fills the pores within cement mortar to form a denser structure, by microbial organic wastewater. Thus, present findings implied a cost-effective of MICP technology to improve the concrete properties along with the mitigation of industrial wastewater pollution, which goes some way towards solving the problem of industrial wastewater pollution.

The removal of arsenic from arsenic-bearing groundwater in In-situ and Ex-situ environment using novel natural magnetic rock material and synthesized magnetic material as adsorbent: A comparative assessment.

The removal of arsenic from groundwater is an important issue for environmental safety and human health. Research focused on the comparative assessment of arsenic removal from arsenic-bearing groundwater and arsenic-containing-synthetic water (2 mg/L) using natural magnetic material (NMM) (rock) and synthesized magnetic material (SMM) by Bacillus pasteurii and humic acid. The arsenic-bearing groundwater (97.56 ±â€¯0.05 µg/L) exceed the WHO limit (10 µg/L) of arsenic concentration for drinking water. The NMM contains dominantly magnetite, hematite, ferrihydrate, coesite, quartz, and stishovite. The NMM of natural rock exhibited the existence of iron (6.25-8.86% Fe3O4), which is widespread and important component in sedimentary rocks. The investigation on vibrating sample magnetometers (VSM) of NMM and SMM demonstrated the typical magnetization properties, which can be separated after arsenic removal process. The thermogravimetric analysis (TGA) of SMM displayed the existence of organic matter decomposition during particle synthesis. The TEM and SEM exhibited the nanoparticle particle formation within the range of 10-39 nm (10-20 nm particle Fe3O4 through B. pasteurii). FTIR spectrum (before and after removal of arsenic) indicated the existence and binding nature in between arsenic and iron. >90% of arsenic was removed from arsenic-bearing groundwater using Fe3O4, Fe3O4 (N2-Environment), Fe3O4 with humic acid, and Fe3O4 with B. pasteurii after 25 min, 8 min, 13 min and 120 min, respectively. In case of NMM in Site-A, the arsenic removal was observed very fast as 85-87% within 30 s, whereas 95-99%, 93-95% and 88-91% removal detected using the sample of Site-A, Site-B, and Site-C respectively, after 120 min at natural pH (8.31 ±â€¯0.05) of arsenic-bearing groundwater. Thus, NMM, (ecofriendly green material), can be applicable for arsenic removal from arsenic-bearing groundwater.

Copper promotes E. coli laccase-mediated TNT biotransformation and alters the toxicity of TNT metabolites toward Tigriopus japonicus.

Although laccase is involved in the biotransformation of 2,4,6-trinitrotoluene (TNT), little is known regarding the effect of E. coli laccase on TNT biotransformation. In this study, E. coli K12 served as the parental strain to construct a laccase deletion strain and two laccase-overexpressing strains. These E. coli strains were used to investigate the effect of laccase together with copper ions on the efficiency of TNT biotransformation, the variety of TNT biotransformation products generated and the toxicity of the TNT metabolites. The results showed that the laccase level was not relevant to TNT biotransformation in the soluble fraction of the culture medium. Conversely, TNT metabolites varied in the insoluble fraction analyzed by thin-layer chromatography (TLC). The insoluble fraction from the laccase-null strain showed fewer and relatively fainter spots than those detected in the wild-type and laccase-overexpressing strains, indicating that laccase expression levels were interrelated determinants of the varieties and amounts of TNT metabolites produced. In addition, the aquatic invertebrate Tigriopus japonicus was used to assess the toxicity of the TNT metabolites. The toxicity of the TNT metabolite mixture increased when the intracellular laccase level in strains increased or when purified E. coli recombinant Laccase (rLaccase) was added to the culture medium. Thus, our results suggest that laccase activity must be considered when performing microbial TNT remediation.