Aminoquinoline-anchored polynorbornene for sequential fluorescent sensing of Zn2+ and ATP.

A novel aminoquinoline functionalized norbornene (1) and its ring-opening metathesis polymerization (ROMP) copolymer P1 have been designed and synthesized. The polymer probe P1 can self-assemble nano aggregation in aqueous solution. The fluorescent experiments revealed that both 1 and P1 show a ratiometric fluorescence response toward Zn2+ over other mental ions in Tris-HCl buffer solution, with the polymer probe P1 shows a better photostability and higher binding affinity than that of the small molecular probe 1. Furthermore, the in situ formed P1-Zn2+ ensemble was successfully used as the secondary sensor for ATP. P1 is also successfully used for monitoring intracellular Zn2+ and ATP in living cells.

Identification of differential pathways in papillary thyroid carcinoma utilizing pathway co-expression analysis.

PURPOSE: To identify differential pathways between papillary thyroid carcinoma (PTC) patients and normal controls utilizing a novel method which combined pathway with co-expression network. METHODS: The proposed method included three steps. In the first step, we conducted pretreatments for background pathways and gained representative pathways in PTC. Subsequently, a co-expression network for representative pathways was constructed using empirical Bayes (EB) approach to assign a weight value for each pathway. Finally, random model was extracted to set the thresholds of identifying differential pathways. RESULTS: We obtained 1267 representative pathways and their weight values based on the co-expressed pathway network, and then by meeting the criterion (Weight > 0.0296), 87 differential pathways in total across PTC patients and normal controls were identified. The top three ranked differential pathways were CREB phosphorylation, attachment of GPI anchor to urokinase plasminogen activator receptor (uPAR) and loss of function of SMAD2/3 in cancer. CONCLUSIONS: In conclusion, we successfully identified differential pathways (such as CREB phosphorylation, attachment of GPI anchor to uPAR and post-translational modification: synthesis of GPI-anchored proteins) for PTC using the proposed pathway co-expression method, and these pathways might be potential biomarkers for target therapy and detection of PTC.