ABSTRACT
MSC transplantation has been explored as a new clinical approach to stem cell-based therapies for bone diseases in regenerative medicine due to their osteogenic capability. However, only a small population of implanted MSC could successfully reach the injured areas. Therefore, enhancing MSC migration could be a beneficial strategy to improve the therapeutic potential of cell transplantation. Catharmus tinctorius volatile oil (CTVO) was found to facilitate MSC migration. Further exploration of the underlying molecular mechanism participating in the pro-migratory ability may provide a novel strategy to improve MSC transplantation efficacy. This study indicated that CTVO promotes MSC migration through enhancing ROCK2 mRNA and protein expressions. MSC migration induced by CTVO was blunted by ROCK2 inhibitor, which also decreased myosin light chain (MLC) phosphorylation. Meanwhile, the siRNA for ROCK2 inhibited the effect of CTVO on MSC migration ability and attenuated MLC phosphorylation, suggesting that CTVO may promote BMSC migration via the ROCK2/MLC signaling. Taken together, this study indicates that C. tinctorius volatile oil could enhance MSC migration via ROCK2/MLC signaling in vitro. C. tinctorius volatile oil-targeted therapy could be a beneficial strategy to improve the therapeutic potential of cell transplantation for bone diseases in regenerative medicine.
Subject(s)
Carthamus tinctorius/chemistry , Cell Movement/drug effects , Mesenchymal Stem Cells/drug effects , Myosin Light Chains/metabolism , Oils, Volatile/pharmacology , rho-Associated Kinases/metabolism , Animals , Cell Proliferation/drug effects , Cell Survival , Cells, Cultured , Male , Mesenchymal Stem Cells/metabolism , Mesenchymal Stem Cells/pathology , Myosin Light Chains/genetics , Oils, Volatile/chemistry , Phosphorylation , Plant Extracts/chemistry , Plant Extracts/pharmacology , Rats, Sprague-Dawley , Signal Transduction/drug effects , rho-Associated Kinases/antagonists & inhibitors , rho-Associated Kinases/geneticsABSTRACT
Hydrothermal reactions of aromatic terphenyl-4,2'',5'',4'-tetracarboxylic acid (H4tta) and transitional metal cations in the presence of two flexible N-donor ancillary ligands afforded four novel coordination polymers, namely, {[Zn(tta)0.5(m-bimb)]·H2O}n (1), [Ni2(tta)(m-bimb)2(H2O)]n (2), [Ni(H2tta)(o-bimb)·H2O]n (3), and [Cd(tta)0.5(o-bimb)]n (4), from solvothermal reactions in the presence of bis(imidazole) bridging linkers (m-bimb = 1,3-bis(imidazol-1-ylmethyl)benzene, and o-bimb = 1,2-bis(imidazol-1-ylmethyl)benzene). Their structures were determined by single-crystal X-ray diffraction analyses and further characterized by elemental analyses (EA), powder X-ray diffraction (PXRD), and thermogravimetric (TG) analyses. Compound 1 displayed a 2-fold 3D â 3D parallel entangled (4,4)-connected bbf net with the point symbol of (64·82)(66)2. Compound 2 featured a predocumented 3D (4,8)-connected (32·53·6)(34·44·59·611) net. Compound 3 exhibited a 2D 4-connected sql sheet with the point symbol of (44·62), and compound 4 showed a 2D 4-connected (32·62·72) kgm sheet. To our delight, fluorescence measurements showed that compound 1 could selectively and sensitively detect Cr3+ cations and nitrobenzene derivatives (nitrobenzene (NB), p-nitrotoluene (PNT), and p-nitroaniline (PNA)), which suggested that CPs of 1 was a promising bifunctional luminescence sensor material that could sense metal ions and small organic molecules. Moreover, 1 showed excellent photocatalytic activity.
