Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 4 de 4
Filter
Add more filters










Database
Language
Publication year range
1.
Natl Sci Rev ; 8(7): nwaa236, 2021 Jul.
Article in English | MEDLINE | ID: mdl-34691688

ABSTRACT

The minimized diffusion limitation and completely exposed strong acid sites of the ultrathin zeolites make it an industrially important catalyst especially for converting bulky molecules. However, the structure-controlled and large-scale synthesis of the material is still a challenge. In this work, the direct synthesis of the single-layer MWW zeolite was demonstrated by using hexamethyleneimine and amphiphilic organosilane as structure-directing agents. Characterization results confirmed the formation of the single-layer MWW zeolite with high crystallinity and excellent thermal/hydrothermal stability. The formation mechanism was rigorously revealed as the balanced rates between the nucleation/growth of the MWW nanocrystals and the incorporation of the organosilane into the MWW unit cell, which is further supported by the formation of MWW nanosheets with tunable thickness via simply changing synthesis conditions. The commercially available reagents, well-controlled structure and the high catalytic stability for the alkylation of benzene with 1-dodecene make it an industrially important catalyst.

2.
Int J Ophthalmol ; 14(9): 1334-1344, 2021.
Article in English | MEDLINE | ID: mdl-34540608

ABSTRACT

AIM: To illustrate the underlying mechanism how prominin-1 (also known as Prom1) mutation contribute to progressive photoreceptor degeneration. METHODS: A CRISPR-mediated Prom1 knockout (Prom1-KO) mice model in the C57BL/6 was generated and the photoreceptor degeneration phenotypes by means of structural and functional tests were demonstrated. Immunohistochemistry and immunoblot analysis were performed to reveal the localization and quantity of related outer segment (OS) proteins. RESULTS: The Prom1-KO mice developed the photoreceptor degeneration phenotype including the decreased outer nuclear layer (ONL) thickness and compromised electroretinogram amplitude. Immunohistochemistry analysis revealed impaired trafficking of photoreceptor OS proteins. Immunoblot data demonstrated decreased photoreceptor OS proteins. CONCLUSION: Prom1 deprivation causes progressive photoreceptor degeneration. Prom1 is essential for maintaining normal trafficking and normal quantity of photoreceptor OS proteins. The new light is shed on the pathogenic mechanism underlying photoreceptor degeneration caused by Prom1 mutation.

3.
Biosens Bioelectron ; 100: 526-532, 2018 Feb 15.
Article in English | MEDLINE | ID: mdl-28988027

ABSTRACT

A novel label-free ratiometric fluorescent probe is developed for the detection of H2O2 based on GelRed/[G3T]5/Tb3+ hybrid, in which GelRed (a nucleic acid dye) intercalated into the designed single-stranded DNA [G3T]5 (i.e. GelRed/[G3T]5) acts as a stable build-in reference with red emission and Tb3+ sensitized by [G3T]5 (i.e. [G3T]5/Tb3+) as a sensitive response signal with green fluorescence. With the successive addition of Hg2+ and cysteine (Cys) to GelRed/[G3T]5/Tb3+, the fluorescence of [G3T]5/Tb3+ can be effectively quenched and recovered respectively, while the fluorescence of GelRed/[G3T]5 remains unchangeable. Combined with these properties, we have demonstrated its application for label-free ratiometric fluorescence detection of H2O2 with self-calibration. The sensing mechanism is based on the specific reaction between H2O2 and Cys, the resulting disulfide reverses the Cys-mediated fluorescence changes of [G3T]5/Tb3+. This method is further applied to the monitoring of oxidase-related reactions. As the glucose oxidase (GOx)-biocatalyzed oxidation of glucose and the acetylcholine esterase/choline oxidase (AChE/ChOx) cascade yield H2O2, such two biocatalytic processes are successfully examined utilized our proposed method, indicating its potential broad applications in biomedical analysis. In addition, we have also demonstrated its feasibility for oxidase-based visual biosensing, in which only a handheld UV lamp is used. Importantly, coupled with our proposed visual biosensing, a realtime quantitative scanometric assays has been also demonstrated by using a smartphone with easy-to-access color-scanning APP as the detection platform. Compared to traditional methods, the proposed design is cost-effective, simple to prepare and easy-to-use without fluorescence labeling or chemical modification.


Subject(s)
Biosensing Techniques/methods , DNA, Single-Stranded/chemistry , Fluorescent Dyes/chemistry , Hydrogen Peroxide/analysis , Terbium/chemistry , Alcohol Oxidoreductases/chemistry , Base Sequence , Cysteine/chemistry , Fluorescence , Glucose Oxidase/chemistry , Humans
4.
Anal Chem ; 88(23): 11647-11653, 2016 12 06.
Article in English | MEDLINE | ID: mdl-27934126

ABSTRACT

A simple and novel method for evaluating antioxidants in complex biological fluids has been developed based on the interaction of dye-labeled single-strand DNA (ssDNA) and polydopamine (PDA). Due to the interaction between ssDNA and PDA, the fluorescence of dye-labeled ssDNA (e.g., FITC-ssDNA, as donor) can be quenched by PDA (as acceptor) to the fluorescence "off" state through Förster resonance energy transfer (FRET). However, in the presence of various antioxidants, such as glutathione (GSH), ascorbic acid (AA), cysteine (Cys), and homocysteine (Hcys), the spontaneous oxidative polymerization reaction from DA to PDA would be blocked, resulting in the freedom of FITC-ssDNA and leading to the fluorescence "on" state. The sensing system shows great sensitivity for the monitoring of antioxidants in a fluorescent "turn on" format. The new strategy also exhibits great selectivity and is free from the interferences of amino acids, metal ions and the biological species commonly existing in brain systems. Moreover, by combining the microdialysis technique, the present method has been successfully applied to monitor the dynamic changes of the striatum antioxidants in rat cerebrospinal microdialysates during the normal/ischemia/reperfusion process. This work establishes an effective platform for in vivo monitoring antioxidants in cerebral ischemia model, and promises new opportunities for the research of brain chemistry, neuroprotection, physiological, and pathological events.


Subject(s)
Antioxidants/analysis , Cerebral Cortex/chemistry , DNA/chemistry , Fluorescent Dyes/chemistry , Indoles/chemistry , Polymers/chemistry , Animals , Ascorbic Acid/analysis , Brain Ischemia , Cysteine/analysis , Glutathione/analysis , Homocysteine/analysis , Rats , Spectrometry, Fluorescence
SELECTION OF CITATIONS
SEARCH DETAIL
...