H2S Prevents Cyclosporine A-Induced Vasomotor Alteration in Rats.

Cyclosporine A (CsA) induces hypertension after transplantation. Hydrogen sulfide (H2S) was found to have hypotensive/vasoprotective effects in the cardiovascular system. The present study aims to investigate the role of H2S on CsA-induced vascular function disorder in rats. Rats were subcutaneously injected with CsA 25 mg/kg for 21 days. Blood pressure was measured by the tail-cuff method. Vasomotion was determined using a sensitive myograph. Western blotting and immunohistochemistry were used to quantify the protein expression of endothelin type A (ETA) receptor and essential MAPK pathway molecules. Vascular superoxide anion production and serum contents of malondialdehyde were determined. The results showed that sodium hydrosulfide (NaHS), a H2S donor, significantly attenuated the increase of blood pressure and contractile responses, and the upregulation of ETA receptor induced by CsA. In addition, NaHS could restore the CsA decreased acetylcholine-induced vasodilatation. Furthermore, NaHS blocked the CsA-induced elevation of reactive oxygen species level, extracellular signal-regulated kinase and p38 MAPK activities. In conclusion, H2S prevents CsA-induced vasomotor dysfunction. H2S attenuates CsA-induced ETA receptor upregulation, which may be associated with MAPK signal pathways. H2S ameliorates endothelial-dependent relaxation, which may be through antioxidant activity.

The expression of bitter taste receptors in mesenteric, cerebral and omental arteries.

AIM: Bitter taste is sensed by the bitter taste receptor (TAS2R), which is mainly expressed in the tongue as well as in extra-oral organs, such as the gastrointestinal tract, respiratory tract, brain, heart and testis. This study aimed to investigate whether TAS2R is expressed in the mesenteric, cerebral and omental arteries. MAIN METHODS: The expression levels of TAS2R mRNA and protein were determined by reverse-transcription polymerase chain reaction and Western blotting, respectively. The location of TAS2R was determined by immunofluorescence imaging. TAS2R agonists were used in a sensitive myograph to study the function of TAS2R in arteries. KEY FINDINGS: The mRNA of rat TAS2Rs, including rTAS2R39, rTAS2R40, rTAS2R108, rTAS2R114, rTAS2R130, rTAS2R137, and rTAS2R140, was expressed in rat mesenteric and cerebral arteries, but rTAS2R114 was not expressed in the cerebral arteries. The mRNA of human TAS2Rs, including hTAS2R3, hTAS2R4, hTAS2R7, hTAS2R10, hTAS2R14, hTAS2R39 and hTAS2R40, was expressed in human omental arteries. The TAS2R7 protein was expressed in rat mesenteric and cerebral arteries, as well as in human omental arteries. Immunofluorescence imaging confirmed that TAS2R7 was located in vascular smooth muscle cells and endothelial cells. The TAS2R agonists, chloroquine and quinine relaxed rat mesenteric arteries and cerebral arteries and human omental arteries in a concentration-dependent manner. SIGNIFICANCE: TAS2R is expressed in the arteries of systemic circulation, including rat mesenteric and cerebral arteries and human omental arteries. This study provides evidence that TAS2R do exist in the arteries and may be involved in the mediation of vessel functions.

Ultrasensitive mercury(II) ion detection by europium(III)-doped cadmium sulfide composite nanoparticles.

With the biomolecule glutathione (GSH) as a capping ligand, Eu(3+)-doped cadmium sulfide composite nanoparticles were successfully synthesized through a straightforward one-pot process. An efficient fluorescence energy transfer system with CdS nanoparticles as energy donor and Eu(3+) ions as energy accepter was developed. As a result of specific interaction, the fluorescence intensity of Eu(3+)-doped CdS nanoparticles is obviously reduced in the presence of Hg(2+). Moreover, the long fluorescent lifetime and large Stoke's shift of europium complex permit sensitive fluorescence detection. Under the optimal conditions, the fluorescence intensity of Eu(3+) at 614 nm decreased linearly with the concentration of Hg(2+) ranging from 10 nmol L(-1) to 1500 nmol L(-1), the limit of detection for Hg(2+) was 0.25 nmol L(-1). In addition to high stability and reproducibility, the composite nanoparticles show a unique selectivity towards Hg(2+) ion with respect to common coexisting cations. Moreover, the developed method was applied to the detection of trace Hg(2+) in aqueous solutions. The probable mechanism of reaction between Eu(3+)-doped CdS composite nanoparticles and Hg(2+) was also discussed.

Determination of formaldehyde in aqueous solutions by a novel fluorescence energy transfer system.

An efficient fluorescence energy transfer (FET) system between CePO(4):Tb(3+) nanocrystals as donor and 6-mercapto-5-triazole[4,3-b]-S-tetrazine as acceptor was built. CePO(4):Tb(3+) nanocrystals were synthesized in aqueous solutions, and characterized by transmission electron microscopy, electron diffraction pattern spectroscopy and spectrofluorometry. Under alkaline conditions, 6-mercapto-5-triazole[4,3-b]-S-tetrazine was generated by redox reaction of formaldehyde (HCHO) with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (AHMT) at room temperature. The degree of the overlap was effective for FET between the emission spectrum of CePO(4):Tb(3+) and the absorption spectrum of 6-mercapto-5-triazole[4,3-b]-S-tetrazine. Based on this system, a simple and sensitive fluorescence method for the selective determination of formaldehyde in aqueous solutions was developed. Under optimal conditions, the quenched fluorescence intensity increased linearly with the concentration of HCHO ranging from 1.03 x 10(-9) to 1.03 x 10(-5) mg mL(-1) with a correlation coefficient of 0.9976. The limit of detection was 1.65 x 10(-10) mg mL(-1). Compared with several methods, the proposed method had a wider linear range, higher selectivity and sensitivity. Moreover, analytical application of the method was demonstrated by water samples.

Different edge effects of paclitaxel- and sirolimus-eluting stents on proximal and distal edges in patients with unstable angina: serial intravascular ultrasound analysis.

BACKGROUND: It is unclear whether edge segments have different responses to paclitaxel eluting stent (PES) and sirolimus eluting stent (SES) implantation in patients with unstable angina. This study aimed to compare the different vascular edge responses in patients with unstable angina and single de novo coronary lesion treated with SES and PES. METHODS: Two hundred and fifty-five patients with unstable angina and single de novo lesion were randomly assigned to PES and SES groups. Serial volumetric intravascular ultrasound (IVUS) images were taken immediately after stenting and at an eight-month follow-up. Five-mm edge segments proximal and distal to the stents were analyzed. RESULTS: Baseline characteristics were comparable between the two groups. At proximal-edge segment, the vessel area decreased and the plaque area increased significantly in the PES group as compared with the SES group. A significant net loss of lumen area was found in the PES group (from (11.10 +/- 3.12) mm(2) at baseline to (9.92 +/- 3.59) mm(2) at the follow-up, P < 0.001). At the distal-edge segment, the net loss of lumen area in the PES group (from (7.71 +/- 2.81) mm(2) at baseline to (6.66 +/- 2.29) mm(2) at the follow-up, P < 0.001) was attributed to a significant increase of plaque area. Proximal-edge stenosis was commonly seen in the PES group (20.0%) as compared with the SES group (5.0%, P = 0.001). This correlated with the higher incidence of target lesion revascularization in the PES group (P = 0.03). Subsegmentally, the smallest Delta lumen area was located at 2 mm proximally in both groups, at 0 mm distally in the PES group, and at 1 mm distally in the SES group. CONCLUSIONS: The two groups demonstrated negative remodeling of edge segments. PES was less effective than SES in inhibiting the growth of plaque within the first 1-mm length proximal to the stent.