Intestinal perforation caused by Epstein-Barr virus-positive primary diffuse large B-cell lymphoma in a patient with HIV.

We report the case of a 48-year-old male who presented with right lower abdominal pain and a mass for 2 weeks and got constipation for 5 days. An abdominal CT scan conducted before admission at other hospitals revealed an obstruction in the blind ascending colon, which was suspected to be a malignant tumor. Proctoscopy revealed peritoneal implantation metastasis and multiple pelvic lymph nodes. Physical examination was unremarkable except for multiple lymph node enlargements in the inguinal area, without pain. A whole-body contrast-enhanced FDG-PET/CT revealed lymphoma involvement in the ascending colon, peritoneum, bone marrow, and lymph nodes in multiple regions of the body, with DLBCL as a suspected diagnosis. Pathological findings from the colonoscopy revealed atypical lymphocyte infiltration and Immunostaining indicated the presence of atypical lymphocytes with Ki-67 (90%) and tested positive for CD20, CD19, CD10, and BCL-6. Based on the above findings, stage IV DLBCL was diagnosed. Furthermore, EBV-DNA amplification was positive. The patient received R-CHOP treatment for 2 days before experiencing symptoms of fevers, chills, and abdominal pain. He underwent emergency surgery due to intestinal perforation, and preoperative blood tests revealed HIV-positive. The prognosis for the patient is poor due to sepsis.

Altered functional connectivity within the brain fear circuit in Parkinson's disease with anxiety: A seed-based functional connectivity study.

Objectives: Aimed to investigate whether there are abnormal changes in the functional connectivity (FC) between the amygdala with other brain areas, in Parkinson's disease (PD) patients with anxiety. Methods: Participants were enrolled prospectively, and the Hamilton Anxiety Rating (HAMA) Scale was used to quantify anxiety disorder. Rest-state functional MRI (rs-fMRI) was applied to analyze the amygdala FC patterns among anxious PD patients, non-anxious PD patients, and healthy controls. Results: Thirty-three PD patients were recruited, 13 with anxiety, 20 without anxiety, and 19 non-anxious healthy controls. In anxious PD patients, FC between the amygdala with the hippocampus, putamen, intraparietal sulcus, and precuneus showed abnormal alterations compared with non-anxious PD patients and healthy controls. In particular, FC between the amygdala and hippocampus negatively correlated with the HAMA score (r = -0.459, p = 0.007). Conclusion: Our results support the role of the fear circuit in emotional regulation in PD with anxiety. Also, the abnormal FC patterns of the amygdala could preliminarily explain the neural mechanisms of anxiety in PD.

A novel CEST-contrast nanoagent for differentiating the malignant degree in breast cancer.

Different subtypes of breast cancer (BCC) have variable degrees of malignancy, which is closely related to their extracellular pH (pHe). Therefore, it is increasingly significant to monitor the extracellular pH sensitively to further determine the malignancy of different subtypes of BCC. Here, a l-arginine and Eu3+ assembled nanoparticle Eu3+@l-Arg was prepared to detect the pHe of two breast cancer models (TUBO is non-invasive and 4T1 is malignant) using a clinical chemical exchange saturation shift imaging technique. The experiments in vivo showed that Eu3+@l-Arg nanomaterials could respond sensitively to changes of pHe. In 4T1 models, the CEST signal enhanced about 5.42 times after Eu3+@l-Arg nanomaterials were used to detect the pHe. In contrast, few enhancements of the CEST signal were seen in the TUBO models. This significant difference had led to new ideas for identifying subtypes of BCC with different degrees of malignancy.

The impact of iron deposition on the fear circuit of the brain in patients with Parkinson's disease and anxiety.

Objective: Anxiety is one of the most common psychiatric symptoms of Parkinson's disease (PD), and brain iron deposition is considered to be one of the pathological mechanisms of PD. The objective of this study was to explore alterations in brain iron deposition in PD patients with anxiety compared to PD patients without anxiety, especially in the fear circuit. Methods: Sixteen PD patients with anxiety, 23 PD patients without anxiety, and 26 healthy elderly controls were enrolled prospectively. All subjects underwent neuropsychological assessments and brain magnetic resonance imaging (MRI) examinations. Voxel-based morphometry (VBM) was used to study morphological brain differences between the groups. Quantitative susceptibility mapping (QSM), an MRI technique capable of quantifying susceptibility changes in brain tissue, was used to compare susceptibility changes in the whole brain among the three groups. The correlations between brain susceptibility changes and anxiety scores quantified using the Hamilton Anxiety Rating Scale (HAMA) were compared and analyzed. Results: PD patients with anxiety had a longer duration of PD and higher HAMA scores than PD patients without anxiety. No morphological brain differences were observed between the groups. In contrast, voxel-based and ROI-based QSM analyses showed that PD patients with anxiety had significantly increased QSM values in the medial prefrontal cortex, anterior cingulate cortex, hippocampus, precuneus, and angular cortex. Furthermore, the QSM values of some of these brain regions were positively correlated with the HAMA scores (medial prefrontal cortex: r = 0.255, p = 0.04; anterior cingulate cortex: r = 0.381, p < 0.01; hippocampus: r = 0.496, p < 0.01). Conclusion: Our findings support the idea that anxiety in PD is associated with iron burden in the brain fear circuit, providing a possible new approach to explaining the potential neural mechanism of anxiety in PD.

A predictive nomogram for intracerebral hematoma expansion based on non-contrast computed tomography and clinical features.

PURPOSE: To develop and validate a new nomogram utilizing non-contrast computed tomography (NCCT) signs and clinical factors for predicting hematoma expansion (HE) in patients with spontaneous intracerebral hemorrhage (ICH). METHODS: HE was defined as > 6 mL or 33% increase in baseline hematoma volume. Multivariable logistic regression analysis was performed to identify the predictors of HE. The discriminatory performance of the proposed model was evaluated via receiver operation characteristic (ROC) analysis, and the predictive accuracy was assessed by a calibration curve. The nomogram was established by R programming language. The decision curve analysis and clinical impact curve were drawn according to the related risk factors. RESULTS: A total of 506 patients with spontaneous ICH were recruited in the development cohort, and 103 patients were registered as the external validation cohort. Among the development cohort, 132 (26.09%) experienced HE. Glasgow coma scale (GCS) (P < 0.001), neutrophil to lymphocyte ratio (NLR) (P < 0.001), blend sign (P < 0.001), swirl sign (P < 0.001), and hypodensities (P = 0.003) were significant predictors of HE, by which were used to establish the nomogram. The model demonstrated good performance with high area under the curve both in the development (AUC = 0.908; 95% confidence interval, 0.880-0.936) and the external validation (AUC = 0.844; 95% confidence interval, 0.760-0.908) cohort. The calibration curve illustrated a high accuracy for HE prediction. CONCLUSION: The nomogram derived from NCCT markers and clinical factors outperformed the NCCT signs-only model in predicting HE for patients with ICH, thus providing an effective and noninvasive tool for the risk stratification of HE.

Mn-Fe3O4 nanoparticles anchored on the urushiol functionalized 3D-graphene for the electrochemical detection of 4-nitrophenol.

Preparation of highly active and cost-effective electrode materials is of great interest in electrochemical detection. In this study, a simple urushiol-templated solvothermal method combined with calcination was proposed to fabricate N-doped three-dimensional graphene (3D-G) with Mn-doped Fe3O4 nanoparticles loaded on the surface (Mn-Fe3O4/3D-G). Because of the large active surface area, porous channel and high loading ratio of Mn-Fe3O4 nanoparticles, as-prepared Mn-Fe3O4/3D-G sensor showed high activity on the determination of 4-nitrophenol (4-NP), which are much improved from the control un-modified samples. The wide linear concentration range (5-100 µM), low detection limit (19 nM) and satisfactory recovery of 4-NP in various water samples (98.38-100.41%) indicated that the Mn-Fe3O4/3D-G electrode can be potentially used for real-world applications. This study gives a simple but meaningful strategy for constructing transition metal oxide/graphene composite materials with high electrocatalytic activity.

In situ UV-visible spectroscopic measurements of kinetic parameters and active sites for catalytic oxidation of alkanes on vanadium oxides.

In situ diffuse reflectance UV-visible spectroscopy was used to measure the dynamics of catalyst reduction and oxidation during propane oxidative dehydrogenation (ODH) on VOx/gamma-Al2O3. Transients in UV-visible intensity in the near-edge region were analyzed using a mechanistic model of ODH reactions. Rate constants per site for the kinetically relevant reduction step (C-H bond activation) measured using this analysis are slightly larger than those obtained from steady-state ODH rates normalized by surface V. The ratio of these values provides a measure of the fraction of the V surface sites that are active for ODH (0.6-0.7, for V surface densities of 2.3-34 V nm(-2)). This suggests that some of the V atoms are either inaccessible or inactive. Reoxidation rate constants, which cannot be obtained from steady-state analysis, are 10(3)-10(5) times larger than those for the C-H bond activation reduction step.

In situ UV-visible assessment of extent of reduction during oxidation reactions on oxide catalysts.

The extent of reduction of active centers during oxidative alkane dehydrogenation on VOx/Al2O3 was measured from pre-edge UV-visible spectral features and found to increase with increasing VOx domain size and propane/O2 ratio.