Deefgea salmonis sp. nov., isolated from gills of rainbow trout (Oncorhynchus mykiss).

A Gram-stain-negative, milky white, aerobic, rod-shaped bacterium named strain H3-26T was isolated from gills of Oncorhynchus mykiss in Lhasa, Tibet Autonomous Region, PR China. Strain H3-26T grew at 4-30 °C and pH 5.0-11.0 (optimum, 25 °C and pH 7.0) with 0-1% (w/v) NaCl (optimum, 0%). The 16S rRNA gene sequence of strain H3-26T showed the highest similarity to Deefgea rivuli WB 3.4-79T (98.42%), followed by Deefgea chitinilytica Nsw-4T (96.91%). Phylogenetic analysis based on 16S rRNA genes indicated that strain H3-26T was a new member of the genus Deefgea. The digital DNA-DNA hybridization and average nucleotide identity values between the genome sequence of strain H3-26T and Deefgea spp. were 21.2-21.9% and 76.3-77.4%, respectively. The genomic DNA G+C content of strain H3-26T was 48.74%. The predominant fatty acids were C12:0, C14:0, C16:0 and C16:1 ω7c. Based on phenotypic, phylogenetic, and genotypic data, strain H3-26T is considered to represent a novel species of the genus Deefgea, for which the name Deefgea salmonis sp. nov. is proposed. The type strain is H3-26T (= JCM 35050T = CICC 25103T).

[Gynecologic cancers in a hospital: a retrospective analysis of admission data over ten years].

OBJECTIVE: To investigate admission patterns of patients with gynecologic cancers over a ten year period, which will provide a basis for further epidemiological studies. METHODS: We reviewed medical records of patients with gynecologic cancers who were admitt d to the West China Second University Hospital of Sichuan University from 2003 to 2012. Their clinicopathological data were extracted and analysed. RESULTS: The number of admitted patients increased over the years, with cervical, uterine and ovary cancers as the top three gynaecological cancers. They accounted for 92.13% of total gynaecological cancers. The peak age of gynaecological cancers was 40-49 years, which accounted for 34.02% (3132/9207) of all patients, followed by 50-59 years (26.64%, 2453/9207). Most (72.46%, 3062/4226) cervical cancer patients aged 30-49 years, compared with 40-59 years for uterine cancers (69.77%, 1768/2534) and 40-59 years for ovarian cancers (58.30%, 1004/1722). Patients in their 20th account for 4.43% (408/9 207) of total cancers, with in which cervical and ovarian cancers as the most common pathological type. Patients under 20 years of age accounted for only 0.98% (90/9207) of total cancers, with ovarian cancers as the most common pathological type. Patients over 60 years accounted for 12.90% (1188/9207) of total cancers, with uterine and ovarian cancers as the most common pathological type. Most patients were at an early stage of cancers when they were admitted to the hospital. CONCLUSION: Hospitalized patients with gynecologic cancers increase over years. Cervical, uterine and ovary cancers remain to be a focus of treatment. Peak age of those cancers varies.