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Guang Pu Xue Yu Guang Pu Fen Xi ; 25(12): 2012-5, 2005 Dec.
Article in Chinese | MEDLINE | ID: mdl-16544495

ABSTRACT

The Raman spectra of DNA in different levels of vitamin C with 10 min ultraviolet radiation and 30 min ultraviolet radiation were reported. The intensity of ultraviolet radiation was 18.68 W x m(-2). The experimental results proved that vitamin C can reduce DNA damage solely, but the effects are related to the concentration of vitamin C. When the concentration of vitamin C was lower than 0.35 mmol x L(-1), DNA was protected better with the rising of vitamin C concentration during ultraviolet radiation. DNA was protected best when vitamin C was 0.35 mmol x L(-1). When vitamin C concentration was higher than 0.35 mmol x L(-1), vitamin C accelerated DNA damage with 10 min ultraviolet radiation. But DNA was also protected with 30 min ultraviolet radiation as vitamin C was 0.35-0.65 mmol x L(-1). However, DNA was only protected slightly at high concentration of vitamin C.


Subject(s)
Ascorbic Acid/chemistry , DNA/chemistry , Ultraviolet Rays , Animals , Cattle , DNA Damage , Spectrum Analysis, Raman
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