The effect of Aceria litchii (Keifer) infestation on the surface properties of litchi leaf hosts.

BACKGROUND: The wettability of target crop surfaces affects pesticide wetting and deposition. The structure and properties of the leaf surface of litchi leaves undergo severe changes after infestation by Aceria litchii (Keifer). The objective of this study was to systematically investigate the surface texture and wettability of litchi leaves infested. RESULTS: Firstly, the investigation focused on the surface structure and physicochemical properties of litchi leaves infested with Aceria litchii. Subsequently, different levels of Contact Angle (CA) were measured individually on the infested litchi leaves. Lastly, Surface Free Energy (SFE) and its polar and dispersive components were calculated using the Owens-Wendt-Rabel-Kaelble (OWRK) method. The outcomes revealed distinctive 3D surface structures of the erineum at various stages of mycorrhizal growth. At stage NO. 1, the height of the fungus displayed a peaked appearance, with the skewness value indicating a surface characterized by more crests. In contrast, at stages NO. 2 and NO. 3, the surface appeared relatively flat. Furthermore, post-infestation of litchi leaves, the CA of droplets on the abaxial surface of diseased leaves exhibited an increase, while the SFE value on the abaxial surface of leaves decreased significantly, in contrast to the abaxial surface of healthy leaves. CONCLUSION: The infestation behavior of Aceria litchii changed the surface structure and chemistry of litchi leaves, which directly affected the CA value of foliar liquids and the SFE value of leaves, changing the surface wettability of litchi leaves from hydrophobic to superhydrophobic. This study provides useful information for improving the wetting and deposition behavior of liquid droplets on the surface of infested leaves. © 2024 Society of Chemical Industry.

Evaluation of aerial spraying application of multi-rotor unmanned aerial vehicle for Areca catechu protection.

Multi-rotor unmanned aerial vehicle (UAV) is a new chemical application tool for tall stalk tropical crop Areca catechu, which could improve deposit performance, reduce operator healthy risk, and increase spraying efficiency. In this work, a spraying experiment was carried out in two A. catechu fields with two leaf area index (LAI) values, and different operational parameters were set. Spray deposit quality, spray drift, and ground loss were studied and evaluated. The results showed that the larger the LAI of A. catechu, the lesser the coverage of the chemical deposition. The maximum coverage could reach 4.28% and the minimum 0.33%. At a flight speed of 1.5 m/s, sprayed droplets had the best penetration and worst ground loss. The overall deposition effect was poor when the flight altitudes were greater than 11.09 m and the flight speed was over 2.5 m/s. Comparing flight speed of 2.5 to 1.5 m/s, the overall distance of 90% of the total drift increased to double under the same operating parameters. This study presents reference data for UAV chemical application in A. catechu protection.

Control Efficacy and Deposition Characteristics of an Unmanned Aerial Spray System Low-Volume Application on Corn Fall Armyworm Spodoptera frugiperda.

As a major global pest, fall armyworm (FAW), Spodoptera frugiperda, invaded China in 2019, which has seriously threatened the safety of China's food production and raised widespread concerns. As a new low-volume application technology, an unmanned aerial spray system (UASS) is playing an important role in the control of FAW in China. However, the studies on the effect of the water application volume on the efficacy of FAW using UASS have been limited. In this study, Kromekote® cards were used to sample the deposition. The method of using a sampling pole and sampling leaf for the determination of deposition. Four water application volumes (7.5, 15.0, 22.5, and 30.0 L/ha) were evaluated with regard to the corn FAW control efficacy. A blank control was used as a comparison. The control efficacy was assessed at 1, 3, 7, and 14 days after treatment (DAT). The tested results showed that sampling methods have a significant effect on deposition results. The number of spray deposits and coverage on the sampling pole were 35 and 40% higher than those on the sampling leaves, respectively. The deposition and control efficacy gradually increased as the water application volume increased. The control efficacy at 14 DAT under different water application volumes was in the range of 59.4-85.4%. These data suggest that UASS spraying can be used to achieve a satisfying control of FAW, but the control efficacy of the water application volume of 30.0 and 22.5 L/ha did not differ significantly. Considering work efficiency, a water application volume of 22.5 L/ha is recommended for field operation.

Precision Detection of Dense Plums in Orchards Using the Improved YOLOv4 Model.

The precision detection of dense small targets in orchards is critical for the visual perception of agricultural picking robots. At present, the visual detection algorithms for plums still have a poor recognition effect due to the characteristics of small plum shapes and dense growth. Thus, this paper proposed a lightweight model based on the improved You Only Look Once version 4 (YOLOv4) to detect dense plums in orchards. First, we employed a data augmentation method based on category balance to alleviate the imbalance in the number of plums of different maturity levels and insufficient data quantity. Second, we abandoned Center and Scale Prediction Darknet53 (CSPDarknet53) and chose a lighter MobilenetV3 on selecting backbone feature extraction networks. In the feature fusion stage, we used depthwise separable convolution (DSC) instead of standard convolution to achieve the purpose of reducing model parameters. To solve the insufficient feature extraction problem of dense targets, this model achieved fine-grained detection by introducing a 152 × 152 feature layer. The Focal loss and complete intersection over union (CIOU) loss were joined to balance the contribution of hard-to-classify and easy-to-classify samples to the total loss. Then, the improved model was trained through transfer learning at different stages. Finally, several groups of detection experiments were designed to evaluate the performance of the improved model. The results showed that the improved YOLOv4 model had the best mean average precision (mAP) performance than YOLOv4, YOLOv4-tiny, and MobileNet-Single Shot Multibox Detector (MobileNet-SSD). Compared with some results from the YOLOv4 model, the model size of the improved model is compressed by 77.85%, the parameters are only 17.92% of the original model parameters, and the detection speed is accelerated by 112%. In addition, the influence of the automatic data balance algorithm on the accuracy of the model and the detection effect of the improved model under different illumination angles, different intensity levels, and different types of occlusions were discussed in this paper. It is indicated that the improved detection model has strong robustness and high accuracy under the real natural environment, which can provide data reference for the subsequent orchard yield estimation and engineering applications of robot picking work.

Numerical simulation and verification of rotor downwash flow field of plant protection UAV at different rotor speeds.

In aerial spraying of plant protection UAVs, the continuous reduction of pesticides is an objective process. Under the condition of constant flight state (speed and altitude), the change of pesticide loading will inevitably lead to the shift of lift force and rotor speed generated by UAV rotor rotation, which will change the distribution of the rotor flow field and affect the effect of aerial spraying operation of plant protection UAV. Therefore, the rotor speed of UAV is taken as the research object in this paper, and the adaptive refinement physical model based on the Lattice Boltzmann Method (LBM) is used to numerically simulate the rotor flow field of the quadrotor plant-protection UAV at different speeds. A high-speed particle image velocimeter (PIV) was used to obtain and verify the motion state of the droplets emitted from the fan nozzle in the rotor flow field at different speeds. The results show that, with the increase of rotor speed, the maximum velocity and vorticity of the wind field under the rotor increase gradually, the top wind speed can reach 13m/s, and the maximum vorticity can reach 589.64s -1. Moreover, the maximum velocity flow value is mainly concentrated within 1m below the rotor, and the maximum vorticity value is primarily concentrated within 0.5m. However, with the increase of time, the ultimate value of velocity and vorticity decreases due to the appearance of turbulence, and the distribution of velocity and vorticity are symmetrically distributed along the centre line of the fuselage, within the range of (-1m, 1m) in the X direction. It is consistent with the motion state of droplets under the action of the rotor downwash flow field obtained by PIV. The study results are expected to reveal and understand the change law of the rotor flow field of plant protection UAVs with the dynamic change of pesticide loading to provide a theoretical basis for the development of precise spraying operation mode of plant protection UAVs and improve the operation effect.

Biological control technology and application based on agricultural unmanned aerial vehicle (UAV) intelligent delivery of insect natural enemies (Trichogramma) carrier.

BACKGROUND: Agricultural pests and diseases affect grain yield and quality. In addition to the use of chemical agents, the biological control of diseases and insect pests has attracted more and more attention due to an urgent need for environmental protection. However, traditional operational methods do not integrate well with biological control technology. With the advantages of simple operation, low operating cost, high operating efficiency and wide application range, use of multirotor unmanned aerial vehicles (UAVs) in biological control is of great significance. RESULTS: An intelligent delivery system comprising an insect natural enemy (Trichogramma) capsule based on an M45 electric multirotor UAV was built to release Trichogramma ostriniae in corn fields under full autonomous mode. During operation, the M45 UAV can fly accurately in accordance with operational requirements; average deviation between the actual and planned routes was 0.24 m, the average flight speed was 9.18 m s-1 and the average altitude was 5.08 m. The effective coverage rate of natural enemies was 99.33% and the coverage redundancy was 36.75%. After delivery, an investigation into the field control effect showed an average control effect of 83.70%, which can result in effective pest control. CONCLUSION: A natural enemy carrier intelligent delivery system based on an M45 electric multirotor UAV was found to have good flight performance. In addition, natural enemies were evenly distributed, giving even coverage of the target fields. The system had a good control effect. © 2021 Society of Chemical Industry.

Field evaluation of spray drift and environmental impact using an agricultural unmanned aerial vehicle (UAV) sprayer.

Unmanned Aerial Vehicle (UAV) applications at low-volume using fine and very fine size droplets have been adopted in several commercial spray scenarios allowing water-saving and high-efficiency operation in delivery of pesticides. However, spray drift associated with UAV applications, especially for fine droplets generated from spinning disk nozzles, has not been fully understood, raising environmental and regulatory concerns. The objectives of this study were to compare the drift potential of three different volume median diameter (VMD, or Dv0.5) of 100, 150 and 200 µm from a commercial quadcopter equipped with centrifugal nozzles exposed to different wind speeds under field conditions. Prior to field test, the droplet size of the centrifugal nozzle was measured by a laser-diffraction particle-size analyzer. The results showed that the relationship between rotation speed and Dv0.5 agrees with the negative power function. Field tests found that the deposition at 12 m downwind direction decreased by an order of magnitude compared with the average deposition within the in-swath zone. The deposition of almost all the treatments at 50 m downwind is lower than the detection limits of 0.0002 µL/cm2. Based on the results from this study, the drift distance of this specific very popular UAV model is much less than that of manned aerial applicators. Based on the predicted equation (R2 = 0.83), the detected drift amount increased with increasing wind speed and decreasing Dv0.5. This work provides basic information to quantify the effect of wind speeds and droplet sizes on UAV spray drift potential which supports on-going regulatory guideline development for spray buffer zone and drift risk assessment protocols.

Activation of Invariant Natural Killer T Cell Subsets in C57BL/6J Mice by Different Injection Modes of α-galactosylceramide.

Whether different injection modes of α-galactosylceramide (α-GalCer) affect the activation of different subsets of invariant natural killer T (iNKT) cells in different tissues and organs of mice is unclear. This study included healthy control, subcutaneous injection, and intraperitoneal injection groups (n=10 in each group). The subcutaneous and intraperitoneal injection groups were injected with α-Galcer (0.1 mg/kg weight), and then the changes in thymus, spleen, and liver iNKT cell frequencies and subsets were observed. The intraperitoneal injection of α-GalCer could increase the frequency of splenic iNKT cells, but the subcutaneous injection did not affect the frequency. Neither injection had any effect on the frequency of iNKT cells in the thymus and liver. The subcutaneous injection of α-GalCer increased the rate of iNKT2 subsets in the thymus but did not affect the rate of iNKT1 subsets. However, the intraperitoneal injection of α-GalCer did not affect thymus iNKT1 and iNKT2 subsets. Interestingly, the subcutaneous injection of α-GalCer significantly increased the proportion of iNKT1 in the spleen and liver but did not significantly change the proportion of iNKT2. The intraperitoneal injection of α-GalCer significantly increased the rate of iNKT2 in spleen and liver but decreased the rate of iNKT1. Subsets of iNKT1 or iNKT2 cells in the spleen and liver were selectively activated by the subcutaneous or intraperitoneal injection of α-GalCer. It provides a valuable means for treating tumors and certain autoimmune diseases. Further exploration of the activation mechanism may provide new ideas about the development of related vaccines.

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase (G6PI)-Induced RA Mice.

Rheumatoid arthritis (RA) is a complex chronic inflammatory autoimmune disease. The pathogenesis of the disease is related to invariant natural killer T (iNKT) cells. Patients with active RA present fewer iNKT cells, defective cell function, and excessive polarization of Th1. In this study, an RA animal model was established using a mixture of hGPI325-339 and hGPI469-483 peptides. The iNKT cells were obtained by in vivo induction and in vitro purification, followed by infusion into RA mice for adoptive immunotherapy. The in vivo imaging system (IVIS) tracking revealed that iNKT cells were mainly distributed in the spleen and liver. On day 12 after cell therapy, the disease progression slowed down significantly, the clinical symptoms were alleviated, the abundance of iNKT cells in the thymus increased, the proportion of iNKT1 in the thymus decreased, and the levels of TNF-α, IFN-γ, and IL-6 in the serum decreased. Adoptive immunotherapy of iNKT cells restored the balance of immune cells and corrected the excessive inflammation of the body.

Subcutaneous administration of α-GalCer activates iNKT10 cells to promote M2 macrophage polarization and ameliorates chronic inflammation of obese adipose tissue.

OBJECTIVE: The role of iNKT cells was investigated in chronic adipose tissue inflammation in obese mice after administration of α-GalCer in different pathways. METHODS: C57BL/6J mice were fed high-fat diet (HFD) for 12 weeks to establish the obese mouse model. The pathology of adipose tissue was observed by H&E staining. The rates of iNKT cells, macrophages and cell subsets in adipose tissue were detected by FCM. Cytokine levels in serum and adipose tissue lymphocyte-stimulated supernatants were assessed with the CBA kit. The expression levels of related transcription factor in adipose tissue were detected by Western blot. RESULTS: The proportions of iNKT cells, iNKT10 cells and M2 macrophages were decreased, while those of iNKT1 and M1 macrophages were increased in adipose tissue of HFD-fed mice. The expression levels of the related transcriptional proteins E4BP4 and Arg-1 were decreased while iNOS expression was increased in adipose tissue. Administration of α-GalCer by subcutaneous injection resulted in increased rates of iNKT10 cells and M2 macrophages, and decreased amounts of M1 macrophages in adipose tissue of HFD-fed mice. The expression of E4BP4 and Arg-1 were up-regulated, but iNOS was down-regulated. Meanwhile, infiltration of inflammatory cells into adipose tissue was further reduced. CONCLUSION: The imbalance between the proportions of iNKT1 and iNKT10 cells may be involved in the development of chronic inflammation in obese adipose tissue. Administration of α-GalCer by subcutaneous injection in HFD-fed mice activates adipose tissue iNKT10 cells, which promote M2 macrophage polarization and improve chronic inflammation in obese adipose tissue.

Methylation of H3K27 and H3K4 in key gene promoter regions of thymus in RA mice is involved in the abnormal development and differentiation of iNKT cells.

Epigenetic modifications have been shown to be important for immune cell differentiation by regulating gene transcription. However, the role and mechanism of histone methylation in the development and differentiation of iNKT cells in rheumatoid arthritis (RA) mice have yet to be deciphered. The DBA/1 mouse RA model was established by using a modified GPI mixed peptide. We demonstrated that total peripheral blood, thymus, and spleen iNKT cells in RA mice decreased significantly, while iNKT1 in the thymus and spleen was increased significantly. PLZF protein and PLZF mRNA levels were significantly decreased in thymus DP T cells, while T-bet protein and mRNA were significantly increased in thymus iNKT cells. We found a marked accumulation in H3K27me3 around the promoter regions of the signature gene Zbtb16 in RA mice thymus DP T cells, and an accumulation of H3K4me3 around the promoters of the Tbx21 gene in iNKT cells. The expression levels of UTX in the thymus of RA mice were significantly reduced. The changes in the above indicators were particularly significant in the progressive phase of inflammation (11 days after modeling) and the peak phase of inflammation (14 days after modeling) in RA mice. Developmental and differentiation defects of iNKT cells in RA mice were associated with abnormal methylation levels (H3K27me3 and H3K4me3) in the promoters of key genes Zbtb16 (encoding PLZF) and Tbx21 (encoding T-bet). Decreased UTX of thymus histone demethylase levels resulted in the accumulation of H3K27me3 modification.

Activation of hepatic iNKT2 cells by α-GalCer ameliorates hepatic steatosis induced by high-fat diet in C57BL/6J mice.

The existence of association between the subpopulation of iNKT cells with different functions and nonalcoholic fatty liver disease has not been confirmed. To investigative the role of iNKT cells in the pathogenesis of nonalcoholic fatty liver disease, we established a non-alcoholic fatty liver model by feeding C57BL/6J mice for 12 weeks with a high-fat diet and injecting α-GalCer through different routes to activate hepatic iNKT cells. The liver of the mice fed a high-fat diet (HFD) had severe hepatic steatosis appearance, elevated pro-inflammatory cytokines and reduced anti-inflammatory cytokines in the liver, and high serum levels of TC, LDL, HDL, and ALT. Our results showed that the percentage of iNKT cells in the liver of the HFD-fed mice was lower than that of the control mice. The expression levels of the related transcription factor of T-bet increased but that of GATA-3 decreased in the HFD-fed mice. The administration of α-GalCer by intraperitoneal injection resulted in increasing of hepatic iNKT and iNKT2 cells but decreasing of hepatic iNKT1 cells, and the expression of GATA-3 and anti-inflammatory cytokine (IL-4) was increased in the liver, and hepatic steatosis was ameliorated in the HFD-fed mice. The administration of α-GalCer by subcutaneous injection resulted in a decrease in hepatic iNKT and iNKT2 and an augmentation of hepatic iNKT1 cells. However, hepatic steatosis was not significantly improved. We concluded that the intraperitoneal injection with α-GalCer effectively improved hepatic steatosis, according to increasing the number of hepatic iNKT2 cells. The precise mechanism requires further exploration.

Study of the adoptive immunotherapy on rheumatoid arthritis with Thymus-derived invariant natural killer T cells.

BACKGROUND: The therapeutic effect of adoptive infusion of specific thymus-derived invariant natural killer T (iNKT) cells in a mouse model of rheumatoid arthritis (RA) was observed, and the mechanism of cellular immunotherapy was preliminarily explored. METHODS: Thymus-derived iNKT cells were infused to RA model mice, with α-GalCer as a positive control. Then, ankle swelling was examined, as well as inflammatory cell infiltration to the joint tissue (hematoxylin-eosin [H&E] staining). Flow cytometry (FCM) was used to assess iNKT cell and helper T lymphocyte (Th) subsets. Serum cytokine levels were determined with cytometric bead array (CBA), with protein expression levels of related transcription factors assessed by Western blot. RESULTS: The joint swelling in RA model animals were significantly improved in the cell therapy and α-GalCer positive control groups (P < 0.05). In addition, iNKT frequencies in peripheral blood, the thymus and spleen were increased significantly (P < 0.05). Meanwhile, iNKT1 subset frequencies in the thymus and spleen were decreased, as well as splenic Th1 and Th17 cell subset rates, and serum TNF-α, IFN-γ and IL-6 levels. The rates of iNKT2 and Th2 subsets as well as IL-4 and IL-10 levels were increased (P < 0.05). Thymus GATA-3 and splenic PLZF protein levels were increased (P < 0.05). CONCLUSIONS: Adoptive infusion of thymus-derived iNKT cells exerts therapeutic effects in RA mice by increasing iNKT frequency, altering the proportions of iNKT cell subsets, correcting Th cell subset imbalance and reducing the amounts of inflammatory cytokines.

Knock-in human FGFR3 achondroplasia mutation as a mouse model for human skeletal dysplasia.

Achondroplasia (ACH), the most common genetic dwarfism in human, is caused by a gain-of function mutation in fibroblast growth factor receptor 3 (FGFR3). Currently, there is no effective treatment for ACH. The development of an appropriate human-relevant model is important for testing potential therapeutic interventions before human clinical trials. Here, we have generated an ACH mouse model in which the endogenous mouse Fgfr3 gene was replaced with human FGFR3G380R (FGFR3ACH) cDNA, the most common mutation in human ACH. Heterozygous (FGFR3ACH/+) and homozygous (FGFR3ACH/ACH) mice expressing human FGFR3G380R recapitulate the phenotypes observed in ACH patients, including growth retardation, disproportionate shortening of the limbs, round head, mid-face hypoplasia at birth, and kyphosis progression during postnatal development. We also observed premature fusion of the cranial sutures and low bone density in newborn FGFR3G380R mice. The severity of the disease phenotypes corresponds to the copy number of activated FGFR3G380R, and the phenotypes become more pronounced during postnatal skeletal development. This mouse model offers a tool for assessing potential therapeutic approaches for skeletal dysplasias related to over-activation of human FGFR3, and for further studies of the underlying molecular mechanisms.

Cholesterol glucosylation by Helicobacter pylori delays internalization and arrests phagosome maturation in macrophages.

BACKGROUND/PURPOSE: Helicobacter pylori colonizes the human stomach and contributes to chronic inflammation of the gastric mucosa. H. pylori persistence occurs because of insufficient eradication by phagocytic cells. A key factor of H. pylori, cholesterol-α-glucosyltransferase encoded by capJ that extracts host cholesterol and converts it to cholesteryl glucosides, is important to evade host immunity. Here, we examined whether phagocytic trafficking in macrophages was perturbed by capJ-carrying H. pylori. METHODS: J774A.1 cells were infected with H. pylori at a multiplicity of infection of 50. Live-cell imaging and confocal microscopic analysis were applied to monitor the phagocytic trafficking events. The viability of H. pylori inside macrophages was determined by using gentamicin colony-forming unit assay. The phagocytic routes were characterized by using trafficking-intervention compounds. RESULTS: Wild type (WT) H. pylori exhibited more delayed entry into macrophages and also arrested phagosome maturation more than did capJ knockout mutant. Pretreatment of genistein and LY294002 prior to H. pylori infection reduced the internalization of WT but not capJ-knockout H. pylori in macrophages. CONCLUSION: Cholesterol glucosylation by H. pylori interferes with phagosome trafficking via a lipid-raft and PI3K-dependent manner, which retards engulfment of bacteria for prolonged intracellular survival of H. pylori.