Six-gene prognostic signature for non-alcoholic fatty liver disease susceptibility using machine learning.

BACKGROUND: nonalcoholic fatty liver disease (NAFLD) is a common liver disease affecting the global population and its impact on human health will continue to increase. Genetic susceptibility is an important factor influencing its onset and progression, and there is a lack of reliable methods to predict the susceptibility of normal populations to NAFLD using appropriate genes. METHODS: RNA sequencing data relating to nonalcoholic fatty liver disease was analyzed using the "limma" package within the R software. Differentially expressed genes were obtained through preliminary intersection screening. Core genes were analyzed and obtained by establishing and comparing 4 machine learning models, then a prediction model for NAFLD was constructed. The effectiveness of the model was then evaluated, and its applicability and reliability verified. Finally, we conducted further gene correlation analysis, analysis of biological function and analysis of immune infiltration. RESULTS: By comparing 4 machine learning algorithms, we identified SVM as the optimal model, with the first 6 genes (CD247, S100A9, CSF3R, DIP2C, OXCT 2 and PRAMEF16) as predictive genes. The nomogram was found to have good reliability and effectiveness. Six genes' receiver operating characteristic curves (ROC) suggest an essential role in NAFLD pathogenesis, and they exhibit a high predictive value. Further analysis of immunology demonstrated that these 6 genes were closely connected to various immune cells and pathways. CONCLUSION: This study has successfully constructed an advanced and reliable prediction model based on 6 diagnostic gene markers to predict the susceptibility of normal populations to NAFLD, while also providing insights for potential targeted therapies.

Isolation and Culture of Primary Human Mammary Epithelial Cells.

The mammary gland is a fundamental structure of the breast and plays an essential role in reproduction. Human mammary epithelial cells (HMECs), which are the origin cells of breast cancer and other breast-related inflammatory diseases, have garnered considerable attention. However, isolating and culturing primary HMECs in vitro for research purposes has been challenging due to their highly differentiated, keratinized nature and their short lifespan. Therefore, developing a simple and efficient method to isolate and culture HMECs is of great scientific value for the study of breast biology and breast-related diseases. In this study, we successfully isolated primary HMECs from small amounts of mammary tissue by digestion with a mixture of enzymes combined with an initial culture in 5% fetal bovine serum-DMEM containing the Rho-associated kinase (ROCK) inhibitor Y-27632, followed by culture expansion in serum-free keratinocyte medium. This approach selectively promotes the growth of epithelial cells, resulting in an optimized cell yield. The simplicity and convenience of this method make it suitable for both laboratory and clinical research, which should provide valuable insights into these important areas of study.

Electrodynamic Interference and Induced Polarization in Nanoparticle-Based Optical Matter Arrays.

Optical matter (OM) arrays are self-organizing, ordered arrangements of nanometer- to micrometer-size particles, where interparticle forces are mediated by incident and scattered coherent light. The structures that form and their dynamics depend on the properties (e.g., material, size) of the constituent particles, as well as the incident and scattered light. While significant progress has been made toward understanding how the OM arrays are affected by the phase, polarization, and intensity profile of the incident light, the polarization induced in the particles and the light scattered by OM arrays have received less attention. In this paper, we establish the roles of electrodynamic interference, many-body coupling, and induced-polarization concomitant with the coherent light scattered by OM arrays. Experiments and simulations together demonstrate that the spatial profile and directionality of coherent light scattered by OM arrays in the far field are primarily influenced by interference, while electrodynamic coupling (interactions) and the associated polarization induced in the nanoparticle constituents have a quantitative wavelength-dependent effect on the total amount of light scattered by the arrays. Furthermore, the electrodynamic coupling in silver nanoparticle OM arrays is significantly enhanced by constructive interference and increases superextensively with the number of particles in the array. Particle size, and hence polarizability, also has a significant effect on the strength of the coupling. Finally, we simulate larger hexagonal OM arrays of Ag nanoparticles to demonstrate that the electrodynamic coupling and scattering enhancement observed in small OM arrays develop into surface lattice resonances observed in the infinite array limit. Our work provides insights for designing OM arrays to tune many-body forces and the coherent light that they scatter.

Wavelength encoding spectral imaging based on the combination of deeply learned filters and an RGB camera.

Hyperspectral imaging is a critical tool for gathering spatial-spectral information in various scientific research fields. As a result of improvements in spectral reconstruction algorithms, significant progress has been made in reconstructing hyperspectral images from commonly acquired RGB images. However, due to the limited input, reconstructing spectral information from RGB images is ill-posed. Furthermore, conventional camera color filter arrays (CFA) are designed for human perception and are not optimal for spectral reconstruction. To increase the diversity of wavelength encoding, we propose to place broadband encoding filters in front of the RGB camera. In this condition, the spectral sensitivity of the imaging system is determined by the filters and the camera itself. To achieve an optimal encoding scheme, we use an end-to-end optimization framework to automatically design the filters' transmittance functions and optimize the weights of the spectral reconstruction network. Simulation experiments show that our proposed spectral reconstruction network has excellent spectral mapping capabilities. Additionally, our novel joint wavelength encoding imaging framework is superior to traditional RGB imaging systems. We develop the deeply learned filter and conduct actual shooting experiments. The spectral reconstruction results have an attractive spatial resolution and spectral accuracy.

Response of soil erosion resistance to straw incorporation amount in the black soil region of Northeast China.

Straw incorporation has been considered as an effective environmental management application to improve soil erosion resistance (SER) and organic carbon sequestration. SER is useful to evaluate soil erosion subjected to concentrated flow. Nevertheless, few studies have been performed to examine how SER varied with the amount of straw incorporation on sloping croplands in high latitude and cool regions. In the current study, the fixed bed scouring tests were conducted in a large hydraulic flume using undisturbed soil samples taken from Hebei small watershed in the black soil region of Northeast China. The response of SER to different straw incorporation amounts (0, 1.125, 2.25, 4.5, 6.75, 9.0 and 13.5 t ha-1) was quantified after three months of straw decomposition. The major influencing factors and the corresponding mechanisms were determined. The findings demonstrated that rill erodibility firstly decreased exponentially with straw incorporation amount (R2 = 0.93), while it slightly increased when straw incorporation amount was more than 9.0 t ha-1. Critical shear stress firstly increased logarithmically (R2 = 0.90) and then slightly decreased when the amount exceeded 9.0 t ha-1. Compared to the treatment of 0 t ha-1, rill erodibility reduced by 17.0%-92.8% and critical shear stress increased by 59.6%-127.2% across different treatments of straw incorporation. Rill erodibility had significant and negative correlations with soil organic matter content, aggregate stability, cohesion, root mass density, straw mass density and straw decomposition amount. The key mechanisms for promoting SER were derived by the direct and indirect effects of straw incorporation and its decomposition on soil physicochemical properties and crop roots. The amount of 9.0 t ha-1 was recommended as the optimum amount of straw incorporation in croplands in Northeast China. These findings are useful to understand how soil erosion resistance responds to the amount of straw incorporation and make rational environmental management policy for semi-humid and cool regions.

Systematic analyses of the factors influencing sperm quality in patients with SARS-CoV-2 infection.

To figure out how does SARS-CoV-2 affect sperm parameters and what influencing factors affect the recovery of sperm quality after infection? We conducted a prospective cohort study and initially included 122 men with SARS-CoV-2 infection. The longest time to track semen quality after infection is 112 days and 58 eligible patients were included in our study eventually. We subsequently exploited a linear mixed-effects model to statistically analyze their semen parameters at different time points before and after SARS-CoV-2 infection. Semen parameters were significantly reduced after SARS-CoV-2 infection, including total sperm count (211 [147; 347] to 167 [65.0; 258], P < 0.001), sperm concentration (69.0 [38.8; 97.0] to 51.0 [25.5; 71.5], P < 0.001), total sperm motility (57.5 [52.3; 65.0] to 51.0 [38.5; 56.8], P < 0.001), progressive motility (50.0 [46.2; 58.0] to 45.0 [31.5; 52.8], P < 0.001). The parameters displayed the greatest diminution within 30 days after SARS-CoV-2 infection, gradually recovered thereafter, and exhibited no significant difference after 90 days compared with prior to COVID-19 infection. In addition, the patients in the group with a low-grade fever showed a declining tendency in semen parameters, but not to a significant degree, whereas those men with a moderate or high fever produced a significant drop in the same parameters. Semen parameters were significantly reduced after SARS-CoV-2 infection, and fever severity during SARS-CoV-2 infection may constitute the main influencing factor in reducing semen parameters in patients after recovery, but the effect is reversible and the semen parameters gradually return to normal with the realization of a new spermatogenic cycle.

Effect of salinity on biological nitrogen removal from wastewater and its mechanism.

The nitrogen discharge from saline wastewater will cause significant pollution to the environment. As a high-efficiency and low-cost treatment method, biological treatment has a promising application prospect in the removal of nitrogen from high-salt wastewater. However, the inhibitory effect of high salt on microorganisms increases the difficulty of its treatment. This review discusses the influence of salinity on the nitrogen removal process, considering both traditional and novel biological techniques. Common methods to enhance the effectiveness of biological nitrogen removal processes and their mechanisms of action in engineering practice and research, including sludge acclimation and inoculation of halophilic bacteria, are also introduced. An outlook on the future development of biological nitrogen removal processes for high-salt wastewater is provided to achieve environmentally friendly discharge of high-salt wastewater.

Roles and Regulation of Quorum Sensing of Acidophiles in Bioleaching: A Review.

Bioleaching has gained significant attention as a cost-effective and environmentally friendly approach for extracting metals from low-grade ores and industrial byproducts. The application of acidophiles in bioleaching has been extensively studied. Among the various mechanisms leaching microorganisms utilize, quorum sensing (QS) is pivotal in regulating their life activities in response to population density. QS has been confirmed to regulate bioleaching, including cell morphology, community structure, biofilm formation, and cell metabolism. Potential applications of QS have also been proposed, such as increasing mineral leaching rates by adding signaling molecules. This review is helpful for comprehensively understanding the role of QS in bioleaching and promoting the practical application of QS-based strategies in bioleaching process optimization.

Morusin Alleviates Aortic Valve Calcification by Inhibiting Valve Interstitial Cell Senescence Through Ccnd1/Trim25/Nrf2 Axis.

The senescence of aortic valve interstitial cells (VICs) plays a critical role in the progression of calcific aortic valve disease (CAVD). However, the precise mechanisms underlying the senescence of VICs remain unclear, demanding the identification of a novel target to mitigate this process. Previous studies have highlighted the anti-aging potential of morusin. Thus, this study aimed to explore the therapeutic potential of morusin in CAVD. Cellular experiments reveal that morusin effectively suppresses cellular senescence and cause a shift toward osteogenic differentiation of VICs in vitro. Mechanistically, morusin activate the Nrf2-mediated antiaging signaling pathway by downregulating CCND1 expression and aiding Keap1 degradation through Trim 25. This activation lead to the upregulated expression of antioxidant genes, thus reducing reactive oxygen species production and thereby preventing VIC osteogenic differentiation. In vivo experiments in ApoE-/- mice on a high-fat Western diet demonstrate the positive effect of morusin in mitigating aortic valve calcification. These findings emphasize the antiaging properties of morusin and its potential as a therapeutic agent for CAVD.

Trends in major non-communicable diseases and related risk factors in China 2002-2019: an analysis of nationally representative survey data.

Background: Prevention and control of non-communicable diseases (NCDs) are prioritized in both the Sustainable Development Goal and the Healthy China 2030 Initiatives. Efforts have been devoted to combating NCDs in China. This study examined changes in NCD trajectory. Methods: We described and analyzed the trends in prevalence and control of major NCDs including obesity, hypertension, diabetes, and dyslipidemia and examined selected main behavioral risk factors in China by sex, age group, and residence using nationally representative CDC survey data. Data included were from the China Chronic Disease Risk Factor Surveillance (CCDRFS, 2013 and 2018) and China National Nutrition Survey (CNNS, 2002, 2010-2013, 2015, and 2020). Annual and relative changes in rates were used. Rural-urban ratio of related indicators was assessed. Findings: NCD-attributed deaths increased from 80.0% in 2002 to 86.6% in 2012, and 88.5% in 2019, with cardiovascular diseases, cancer, chronic respiratory diseases, and diabetes accounted for 47.1%, 24.1%, 8.8%, and 2.5% of deaths in 2019, respectively. Prevalence of obesity (7.1%-16.4%), overweight/obesity (29.9%-50.7%), hypertension (18.8%-27.5%), diabetes (2.6%-11.9%), and dyslipidemia (18.6%-35.6%) all increased from 2002 to 2018. These rates increased faster in rural areas than in urban areas. Rates of awareness, treatment and control of hypertension and diabetes increased very slowly from 2012 to 2018. Most rates were between 30 and 40% with the lowest rate of 11% for hypertension control even in 2018. The rates were worse for rural residents compared to urban residents. Furthermore, many modifiable behavioral risk factors showed little improvement and some became worse over time, including smoking, excessive alcohol use, inadequate vegetable/fruit intake, excessive red meat intake, and physical inactivity. Interpretation: NCD burden in China increased during 2002-2019 despite of the intervention efforts. To reach the global and national targets, China must strengthen its actions, especially in rural areas, including improvement of NCD screening and management and reduction of behavioral risk factors. Funding: The study was supported in part by research grants of National Key R&D Program of China (2017YFC0907200, 2017YFC0907201), International Collaboration Project from the Chinese Ministry of Science and Technology-Prevention and control of chronic diseases and health promotion (G2021170007L), Natural Scientific Foundation of China (82103846), Key R&D and Transformation Program of Qinghai (2023-QY-204).

Carbon-Coated Tin-Titanate derived SnO2/TiO2 nanowires as High-Performance anode for Lithium-Ion batteries.

Tin dioxide (SnO2) is a promising alternative material to graphite anode, but the large volume change induced electrode pulverization issue has limited its application in lithium-ion batteries (LIBs). In contrast, titanium dioxide (TiO2) anode shows high structure stability upon lithium insertion/extraction, but with low specific capacity. To overcome their inherent disadvantages, combination of SnO2 with TiO2 and highly conductive carbon material is an effective way. Herein, we report a facile fabrication method of carbon-coated SnO2/TiO2 nanowires (SnO2/TiO2@C) using tin titanate nanowires as precursor, which are prepared by reacting SnCl2·2H2O with layered sodium titanate (Na2Ti3O7) nanowires in the aqueous solution though the ion exchange between Sn2+ and Na+. After annealing under argon atmosphere, the hydrothermally carbon-coated tin-titanate nanowires decompose, forming a unique hybrid structure, where ultrafine SnO2 nanoparticles are uniformly embedded within the TiO2 substrate with carbon coating. Consequently, the SnO2/TiO2@C nanowires demonstrate excellent lithium storage capacity with high pseudocapacitance contribution, excellent reversible capacity, and long-term cycling stability (673.7/510.5 mAh/g at 0.5/1.0 A/g after 250/800 cycles), owing to the unique hybrid structure, as the well-dispersion of ultra-small SnO2 within TiO2 nanowire substrate with simultaneous carbon coating efficiently suppresses the volume changes of SnO2, provides abundant reactive sites for lithium storage, and enhances the electrical conductivity with shortened ion transport distance.

A portable magnetic electrochemical sensor for highly efficient Pb(II) detection based on bimetal composites from Fe-on-Co-MOF.

The practical, sensitive, and real-time detection of heavy metal ions is an essential and difficult problem. This study presents the design of a unique magnetic electrochemical detection system that can achieve real-time field detection. To enhance the electrochemical performance of the sensor, Fe2O3@C-800, Co/CoO@/C-600, and CoFe2O4@C-600 magnetic composites were synthesized using three MOFs precursors by the solvothermal method. And the morphology structure and electrochemical properties of as-prepared magnetic composites were researched by X-ray diffraction (XRD), Scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometer (VSM), specific surface area and porosity analyzer (BET) and differential pulse voltammetry (DPV). The results shown that these composites improve conductivity and stability while preserving the MOFs basic frame structure. Compared with the monometallic MOFs-derived composites, the synergistic effect of the bimetallic composite CoFe2O4@C-600 can significantly enhance the electrochemical performance of the sensor. The linear range for the detection of lead ions was 0.001-60 µM, and the detection limit was 0.0043 µM with a sensitivity of 22.22 µA µM·cm-2 by differential pulse voltammetry. The sensor has good selectivity, stability, reproducibility and can be used for actual sample testing.

Fe3O4-Incorporated Metal-Organic Framework for Chemo/Ferroptosis Synergistic Anti-Tumor via the Enhanced Chemodynamic Therapy.

Metal-organic framework (MOF)-based drug delivery nanomaterials for cancer therapy have attracted increasing attention in recent years. Here, an enhanced chemodynamic anti-tumor therapy strategy by promoting the Fenton reaction by using core-shell zeolitic imidazolate framework-8 (ZIF-8)@Fe3O4 as a therapeutic platform is proposed. Carboxymethyl cellulose (CMC) is used as a stabilizer of Fe3O4, which is then decorated on the surface of ZIF-8 via the electrostatic interaction and serves as an efficient Fenton reaction trigger. Meanwhile, the pH-responsive ZIF-8 scaffold acts as a container to encapsulate the chemotherapeutic drug doxorubicin (DOX). The obtained DOX-ZIF-8@Fe3O4/CMC (DZFC) nanoparticles concomitantly accelerate DOX release and generate more hydroxyl radicals by targeting the lysosomes in cancer cells. In vitro and in vivo studies verify that the DZFC nanoparticles trigger glutathione peroxidase 4 (GPX4)-dependent ferroptosis via the activation of the c-Jun N-terminal kinases (JNK) signaling pathway, following to achieve the chemo/ferroptosis synergistic anti-tumor efficacy. No marked toxic effects are detected during DZFC treatment in a tumor-bearing mouse model. This composite nanoparticle remarkably suppresses the tumor growth with minimized systemic toxicity, opening new horizons for the next generation of theragnostic nanomedicines.

Influence of biochar on the arsenic phytoextraction potential of Pteris vittata in soils from an abandoned arsenic mining site.

Biochar (BC) has a strong potential for activating arsenic (As) in soil; thus, the phytoremediation efficiency of As-polluted soils is enhanced with Pteris vittata L. A pot experiment was conducted to investigate the potential of BC to assist in phytoremediation with P. vittata. The effects of BC on physicochemical properties, available As, enzyme activities, and the bacterial community in the rhizosphere soil were investigated, and the biomass, physiology, and As uptake of P. vittata were analyzed. The results indicated that applying BC facilitated available As in the P. vittata rhizosphere soil, and the phytoremediation efficiency percentage increased in the As-polluted soils, such as 3.80% and 8.01% under the 2% and 5% BC treatments compared to the control, respectively. Phytoremediation with P. vittata and BC significantly improved soil organic matter content, available N, P, and K, enzyme activities, and the bacterial community. BC promoted Streptomyces (26.6-54.2%) and Sphingomonas (12.3-30.8%) abundance which regulated the growth and As uptake by P. vittata. Moreover, applying BC increased the biomass, and As uptake by P. vittata. Overall, BC strengthened the phytoremediation of As-polluted soils by improving soil pH, nutrient concentrations, enzyme activities, bacterial community structure, and soil arsenic activation, growth, and absorption by P. vittata.

Nonylphenol and its derivatives: Environmental distribution, treatment strategy, management and future perspectives.

In recent years, emerging pollutants, including nonylphenol (NP) and nonylphenol ethoxylate (NPE), have become a prominent topic. These substances are also classified as persistent organic pollutants. NP significantly affects the hormone secretion of organisms and exhibits neurotoxicity, which can affect the human hippocampus. Therefore, various countries are paying increased attention to NP regulation. NPEs are precursors of NPs and are widely used in the manufacture of various detergents and lubricants. NPEs can easily decompose into NPs, which possess strong biological and environmental toxicity. This review primarily addresses the distribution, toxicity mechanisms and performance, degradation technologies, management policies, and green alternative reagents of NPs and NPEs. Traditional treatment measures have been unable to completely remove NP from wastewater. With the progressively tightening management and regulatory policies, identifying proficient and convenient treatment methods and a sustainable substitute reagent with comparable product effectiveness is crucial.

Magnetic nanoparticle-based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update.

Mycotoxin contamination of food crops is a global challenge due to their unpredictable occurrence and severe adverse health effects on humans. Therefore, it is of great importance to develop effective tools to prevent the accumulation of mycotoxins through the food chain. The use of magnetic nanoparticle (MNP)-assisted biosensors for detecting mycotoxin in complex foodstuffs has garnered great interest due to the significantly enhanced sensitivity and accuracy. Within such a context, this review includes the fundamentals and recent advances (2020-2023) in the area of mycotoxin monitoring in food matrices using MNP-based aptasensors and immunosensors. In this review, we start by providing a comprehensive introduction to the design of immunosensors (natural antibody or nanobody, random or site-oriented immobilization) and aptasensors (techniques for aptamer selection, characterization, and truncation). Meanwhile, special attention is paid to the multifunctionalities of MNPs (recoverable adsorbent, versatile carrier, and signal indicator) in preparing mycotoxin-specific biosensors. Further, the contribution of MNPs to the multiplexing determination of various mycotoxins is summarized. Finally, challenges and future perspectives for the practical applications of MNP-assisted biosensors are also discussed. The progress and updates of MNP-based biosensors shown in this review are expected to offer readers valuable insights about the design of MNP-based tools for the effective detection of mycotoxins in practical applications.

AVCAPIR: A Novel Procalcific PIWI-Interacting RNA in Calcific Aortic Valve Disease.

BACKGROUND: Calcification of the aortic valve leads to increased leaflet stiffness and consequently results in the development of calcific aortic valve disease (CAVD). However, the underlying molecular and cellular mechanisms of calcification remain unclear. Here, we identified a novel aortic valve calcification-associated PIWI-interacting RNA (piRNA; AVCAPIR) that increases valvular calcification and promotes CAVD progression. METHODS: Using piRNA sequencing, we identified piRNAs contributing to the pathogenesis of CAVD that we termed AVCAPIRs. High-cholesterol diet-fed ApoE-/- mice with AVCAPIR knockout were used to examine the role of AVCAPIR in aortic valve calcification (AVC). Gain- and loss-of-function assays were conducted to determine the role of AVCAPIR in the induced osteogenic differentiation of human valvular interstitial cells. To dissect the mechanisms underlying AVCAPIR-elicited procalcific effects, we performed various analyses, including an RNA pulldown assay followed by liquid chromatography-tandem mass spectrometry, methylated RNA immunoprecipitation sequencing, and RNA sequencing. RNA pulldown and RNA immunoprecipitation assays were used to study piRNA interactions with proteins. RESULTS: We found that AVCAPIR was significantly upregulated during AVC and exhibited potential diagnostic value for CAVD. AVCAPIR deletion markedly ameliorated AVC in high-cholesterol diet-fed ApoE-/- mice, as shown by reduced thickness and calcium deposition in the aortic valve leaflets, improved echocardiographic parameters (decreased peak transvalvular jet velocity and mean transvalvular pressure gradient, as well as increased aortic valve area), and diminished levels of osteogenic markers (Runx2 and Osterix) in aortic valves. These results were confirmed in osteogenic medium-induced human valvular interstitial cells. Using unbiased protein-RNA screening and molecular validation, we found that AVCAPIR directly interacts with FTO (fat mass and obesity-associated protein), subsequently blocking its N6-methyladenosine demethylase activity. Further transcriptomic and N6-methyladenosine modification epitranscriptomic screening followed by molecular validation confirmed that AVCAPIR hindered FTO-mediated demethylation of CD36 mRNA transcripts, thus enhancing CD36 mRNA stability through the N6-methyladenosine reader IGF2BP1 (insulin-like growth factor 2 mRNA binding protein 1). In turn, the AVCAPIR-dependent increase in CD36 stabilizes its binding partner PCSK9 (proprotein convertase subtilisin/kexin type 9), a procalcific gene, at the protein level, which accelerates the progression of AVC. CONCLUSIONS: We identified a novel piRNA that induced AVC through an RNA epigenetic mechanism and provide novel insights into piRNA-directed theranostics in CAVD.

Environmental tobacco smoke increased risk of gestational diabetes mellitus: A birth cohort study in Sichuan, China.

INTRODUCTION: Studies on the relationship between environmental tobacco smoke (ETS) and gestational diabetes mellitus (GDM) are limited. In this study, we aimed to clarify the association between ETS at different trimesters of pregnancy and the risk of GDM among non-smoking pregnant women. METHODS: A total of 16,893 non-smoking mothers from the Southwest Birth Cohort, China, were included in the final analyses. Exposure and outcome measures included self-reported ETS status at different trimesters of pregnancy and GDM diagnosis. Multivariable logistic regression models were constructed to estimate the association between ETS and GDM. RESULTS: The prevalence of ETS exposure was 25.7%. Compared with no ETS, ever ETS had an increased risk of GDM, with an adjusted odds ratio (95% confidence intervals) of 1.21 (1.09, 1.33). The association remained consistent at different trimesters of pregnancy ETS exposure. In the last trimester and with continuous ETS exposure, the risk of GDM increased significantly with the increase in the duration of the exposure. The risk of GDM associated with ever ETS during pregnancy significantly increased in mothers over 30 years old and pre-pregnancy overweight (P for interaction <0.05). CONCLUSIONS: ETS exposure at different trimesters of pregnancy was associated with an increased risk of GDM among non-smoking pregnant women. These findings emphasise the importance of preventing ETS exposure during pregnancy.

Value of adjuvant chemotherapy for patients with pT2N0M0 non-small cell lung cancer receiving radical resection.

BACKGROUND: Associations between adjuvant chemotherapy (ACT) and the improvement in survival for patients with pT2N0M0 non-small cell lung cancer (NSCLC) who received R0 resection remain controversial. This study aimed to evaluate the value of ACT for patients with pT2N0M0 NSCLCs, and to identify the subgroups who could benefit from ACT. METHODS: Multivariable Cox proportional hazards regression models were used to estimate independent prognostic factors. High-risk factor (HRF) included visceral pleural invasion (VPI), lymphovascular invasion (LVI) and poor differentiation/undifferentiated tumors. RESULTS: Of the 899 patients, 277 (30.8%) patients received ACT. More younger patients (p < 0.001) and male patients (p = 0.007) received ACT. With the increase of pathological tumor size (p < 0.001) and the number of HRFs (p < 0.001), there was a significant rise in the proportion of patients receiving ACT. For all patients, ACT could not improve recurrence-free survival (RFS) (p = 0.672) and overall survival (OS) (p = 0.306). For patients with pathological stage IIA or radiological pure-solid tumors, ACT could significantly improve the OS (p = 0.011 and p = 0.037, respectively), and multivariate analysis revealed that ACT was an independent prognostic factor for patients with pathological stage IIA (p = 0.005). ACT could improve the OS significantly in patients with pathological stage IB pure-solid lung adenocarcinoma (LUAD) (p = 0.043). CONCLUSION: ACT was valuable for patients with pathological stage IIA (pT2bN0M0) and patients with radiological pure-solid LUAD of pathological stage IB. A combination of radiological features and pathological subtypes could be helpful when selecting patients with pT2N0M0 NSCLCs for ACT.

Evaluation of Exercise Mobile Applications for Adults with Cardiovascular Disease Risk Factors.

OBJECTIVE: To conduct a systematic review to determine if there are exercise mobile applications (apps) that can produce evidence-based, individualized exercise plans. MATERIALS AND METHODS: We searched the Apple Store and Google Play for exercise apps with terms related to exercise and health. Exercise apps were eligible if they: (1) had a ≥4 out of 5 overall rating with ≥1000 reviews; (2) were free to download; and (3) were not gender specific. Exercise apps were evaluated via the evidence-based exercise prescription (ExRx) standards of the American College of Sports Medicine (ACSM) and American Heart Association. For the exercise app evaluation criteria, an app was included if it (1) was evidence-based; (2) contained a preparticipation health screening protocol; (3) built a cardiovascular disease (CVD) risk factor profile; (4) prioritized one chronic disease or health condition to focus on; (5) framed the exercise plan by the frequency, intensity, time, and type principle (FITT) of ExRx; and (6) specified special considerations. RESULTS: Of the 531 potentially qualifying apps, 219 qualified. The qualifying apps were rarely evidenced-based (0.5%) or had a preparticipation screening protocol (3.7%). Only 27.7% built CVD risk factor profiles. Most apps (64.8%) focused on body image and/or athletic performance. Only 4.3% focused on chronic diseases or health conditions, while the remainder (34.5%) did not disclose a focus. No app framed the exercise plans by the FITT of ExRx. Only 1.4% of the apps specified special considerations. CONCLUSION: There are no commercially available exercise apps that generate an evidence-based, individualized exercise plan for adults with CVD risk factors.