[Application of pressure of end-tidal carbon dioxide concentration in polysomnography].

OBJECTIVE: The purpose of this study was to investigate the end-tidal carbon dioxide concentration (PETCO2) monitoring coupling in polysomnography for patients with obstructive sleep apnea hypopnea syndrome (OSAHS) during sleep. METHODS: PETCO2 was sampled through a Oral-Nasal Cannula and measured using micro-stream capnometer. Capnometer was calibrated according to the manufacturer instructions and integrated into the standard polysomnographic recordings. Thirty-eight consecutive patients underwent overnight polysomnography (PSG) were synchronously monitored PETCO2. All variables were recorded continuously and transferred to a computer for analysis. RESULTS: PETCO2 numeric values and waveform were displayed in real time on the PSG epoch. The mean PETCO2 of wake, non-rapid eye movement, rapid eye movement and TST(?) were negatively correlated with apnea-hypopnea index and arousal index (r were -0.458 ∼ -0.688, P < 0.01), were positively correlated with mean arterial oxygen saturation (SaO2) and lowest SaO2, (r were 0.604 ∼ 0.674, P < 0.01). CONCLUSIONS: The study provides preliminary data showing that PETCO2 potentially can be used in continuous monitoring of OSAHS patients. And PETCO2 can indicate the severity of OSAHS.

[Construction and transfection experiment of a goose circovirus infectious clone].

A pair of primers with BamH I restriction site were designed to amplify the complete genome of goose circovirus. Two copies of the genome were ligated in tandem and cloned into pGEM-T Easy vector to construct an infectious clone named as pGEMT-2GoCV. The pGEMT-2GoCV linearized with EcoR I was transfected to negative embryos and gosling with Lipfectamine. PCR detection verified the proliferation of GoCV in geese. Some sera of the embryo transfected group were detected to be positive at 2 and 4 weeks after hatching and one bursa was detected to be positive at 4 weeks. Some sera of the gosling transfected group were also detected to be positive at 2 weeks after transfection. Furthermore, the mark in the PCR products were identified by BamH I digestion and the GoCV in positive tissue and sera were quantitated by Real-time PCR. The results showed that the virus load in positive bursa was 1.57 x 10(6) copies/mg, the virus load in positive sera were 3.52 x 10(4)-5.92 x 10(5) copies/microL. In conclusion, the infectious DNA clone constructed with two copies of full-length GoCV genome in tandem can transfect embryo and gosling and propagate the marked goose circovirus.

[Expression characteristics of prolactin gene in Eastern Zhejiang white geese].

This study was conducted to clone the prolactin gene (PRL) in Eastern Zhejiang White Geese and to investigate the PRL gene expression characteristics during egg-laying, out-of-lay and incubating periods by real time PCR. Comparisons were made respectively of concentration of prolactin mRNA in the hypothalamus, pituitary gland and ovary of the adult female geese at different reproductive periods. The result indicated that there were significant differences (P<0.05) in PRL mRNA expression between different reproductive periods of the geese. The lowest level of PRL expression was found in out-of-lay geese, higher in the egg-laying geese, and the highest in incubating geese. Furthermore, the analysis of PRL expression in different tissues indicated that the highest levels of PRL was expressed in the pituitary gland, followed in hypothalamus, and the least in ovary of the geese. There were significant difference (P<0.01) expression of PRL between the pituitary gland/hypothalamus and ovary of the geese, whereas no any difference was observed between the pituitary gland and hypothalamus (P>0.05). In summary, the PRL mRNA expression had variance in different reproductive periods of the geese.